neurodevelopment, brainstem, motor neuron, gene expression patterns, axon targeting, congenital cranial dysinnervation disorders (CCDDs), amyotrophic lateral sclerosis (ALS), neuropathology, imaging, bioinformatics, single cell approaches, transcriptomics
-Collaborator-POI, Pathology, Brigham & Women's Hospital (2021-Present)
-Instructor, Harvard Medical School (2016-Present)
-Associated Scientist, Klarman Cell Observatory, The Broad Institute of MIT and Harvard (2016-Present)
-Affiliated Non-Clinical Staff, Engle Lab, F.M. Kirby Neurobiology Center, Boston Children’s Hospital (2021-Present)
-Affiliated Non-Clinical Staff, Engle Lab, Pathology, Boston Children’s Hospital (2014-2020)
-Associate Pathologist, Pathology (Neuropathology), Brigham and Women’s Hospital (2016-2021)
-Research/Clinical Fellow, Pathology, Laboratory of Elizabeth Engle, Boston Children's Hospital and Brigham and Women’s Hospital (2014-2016)
-Affiliated Fellow, Pathology, Boston Children’s Hospital (2014)
-Neuropathology Fellow, Pathology (Neuropathology), Brigham & Women's Hospital, Boston Children’s Hospital, & Beth Israel Deaconess Medical Center (2012-2014)
-Resident, Pathology, Brigham and Women's Hospital (2010-2012)
We study the gene networks driving neuronal diversity during brainstem development to uncover why only specific subsets are differentially affected in a given human neurologic disease. The brainstem controls multiple critical motor and sensory functions, including eye movements, facial expression, speech, hearing, proprioception, arousal, and breathing. Disruption of these functions can lead to profound deficits including in childhood social interaction. The anatomy and gene circuits in the brainstem are highly conserved in mice, providing an ideal model system to investigate neuronal specification and axon growth and guidance in both health and disease. We combine neurodevelopmental tools, 3D imaging, multi-omics/bioinformatic approaches, molecular/synthetic biology, and mouse models of human disease to define developmental differences among various neuronal populations. Through a single cell and spatial transcriptomic atlas of developing motor neurons, we have identified unique genetic fingerprints of each motor neuron population, including novel markers of spatially and temporally distinct subpopulations that contribute to specific aberrant nerve branches in mouse models of childhood disorders. This work provides a new toolbox to study differential neuronal vulnerability in disease.
I’ve pursued research since an early age, supported by many wonderful mentors and teachers along the way, and I consider mentoring and training the next generation of scientists one of my primary goals. I have progressively developed approaches in critical thinking, imaging, cell culture, animal work, bioinformatics, and neuropathology through work in the laboratories of Jeff Corwin, PhD (University of Virginia), Huda Zoghbi, MD and Hugo Bellen, PhD (Baylor College of Medicine), Jeff Lichtman, MD/PhD (The Marine Biological Laboratory), Elizabeth Engle, MD (Harvard Medical School), & Aviv Regev, PhD (The Broad Institute of MIT & Harvard), among many others. I’m also a clinical neuropathologist and look for areas to apply our research findings to medicine.
Rose MF, Tenney AP, Ray TP, Creighton D, Gelber A, Tischfield MA, Murray E, Collins T, Nugent AA, Ang P, Izen S, Bauer MR, Huang W, Satija R, Rozenblatt-Rosen O, Chen F, Macosko EZ, Regev A, Engle EC. (2021). Developing hindbrain motor neurons show spatial and temporal transcriptomic diversity mapping to wiring decisions. In: American Association of Neuropathologists, Inc. Abstracts of the 97th Annual Meeting June 10–13, 2021, St. Louis, MO. Journal of Neuropathology and Experimental Neurology. 80(6):564. (Published Abstract)
An D, Fujiki R, Iannitelli DE, Smerdon SW, Maity S, Wanaselja EK, Yagudayeva I, Lee JY, Rose MF, Vogel C, Wichterle H, Engle EC, Mazzoni EO. (2019) Stem cell-derived cranial and spinal neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons. eLife. 8:e44423.
Di Gioia SA, Connors S*, Matsunami N*, Cannavino J*, Rose MF*, Gilette NM, Artoni P, de Macena Sobreira NL, Chan WM, Webb BD, Robson CD, Cheng L, Van Ryzin C, Ramirez-Martinez A, Mohassel P, Leppert M, Scholand MB, Grunseich C, Ferreira CR, Hartman T, Hayes IM, Morgan T, Markie DM, Fagiolini M, Swift A, Chines PS, Speck-Martins CE, Collins FS, Jabs EW, Bönnemann CG, Olson EN; Moebius Syndrome Research Consortium, Carey JC, Robertson SP, Manoli I, Engle EC. (2017) A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome. Nature Communications. 8:16077. (*equal contribution)
Driver EC, Sillers L, Coate TM, Rose MF, Kelley MW. (2013) The Atoh1-lineage gives rise to hair cells and supporting cells within the mammalian cochlea. Developmental Biology. 376(1):86-98.
Rose MF*, Ren J*, Ahmad KA*, Chao HT, Klisch TJ, Flora A, Greer JJ, Zoghbi HY. (2009) Math1 is essential for the development of hindbrain neurons critical for perinatal breathing. Neuron. 64(3):341-54. (*equal contribution)
Rose MF, Ahmad KA, Thaller C, Zoghbi HY. (2009) Excitatory neurons of the proprioceptive, interoceptive, and arousal hindbrain networks share a developmental requirement for Math1. Proceedings of the National Academy of Sciences. 106(52):22462-7.
England C, Kowalczyk T, Dagan A, Daza RAM, Lau C, Rose MF, Hevner RF. (2006) Unipolar Brush Cells of the Cerebellum are Produced in the Rhombic Lip and Migrate through Developing White Matter. Journal of Neuroscience. 26(36):9184-95.
Chizhikov VV, Lindgren A, Currle DS, Rose MF, Monuki ES, Millen KJ. (2006) The dorsal midline is a signaling center regulating cerebellar neural cell-type specification and proliferation. Development. 133(15):2793-804.
Young JI, Hong EP, Castle JC, Crespo-Barreto J, Bowman AB, Rose MF, Kang D, Richman R, Johnson JM, Berget S, Zoghbi HY. (2005) Regulation of RNA splicing by the methylation-dependent transcriptional repressor MeCP2. Proceedings of the National Academy of Sciences. 102(49):17551-8.
Wang VY*, Rose MF*, Zoghbi HY. (2005) Math1 expression redefines the rhombic lip and reveals novel lineages within the brainstem and cerebellum. Neuron. 48:31-43. (*equal contribution)
Tsuda H, Jafar-Nejad H, Patel AJ, Sun Y, Chen HK, Rose MF, Venken KJT, Botas J, Orr HT, Bellen HJ, Zoghbi HY. (2005) The AXH domain of Ataxin-1 mediates neurodegeneration through its interaction with Gfi-1/Senseless proteins. Cell. 122:633-644.
Frankfort BJ, Pepple KL, Mamlouk M, Rose MF, Mardon G. (2004) Senseless is required for pupal retinal development in Drosophila. Genesis. 38:182-194.
Rose MF, Rose SR. (1998) Melatonin accelerates metamorphosis in Xenopus laevis. J Pineal Res. 24:90-95.
Mapping Brainstem Motor Neuron Subtypes and Genetic Pathways Involved in their Differential Susceptibility to Disease
NIHK08NS099502; Dec 1, 2016 - Nov 30, 2022;
Role: Principal Investigator
Role of Math1 in brain stem &cerebellar development
NIHF31NS051046; Sep 30, 2004 - Oct 31, 2007;
Role: Principal Investigator
Interdepartmental Neuroscience Program
Cellular and Molecular Biosciences
Experimental Pathology (PTH)
Mathematical and Computational Biology
Center for Complex Biological Systems