Associate Professor, Urology
School of Medicine

tissue engineering, urology, large animal surgical models

Jerry D. Choate Presidential Chair in Urology Tissue Engineering

2004-2006 Tufts University, Advisor Dr. David Kaplan

My research focuses on the development of novel tissue engineering strategies for hollow organ repair and regeneration. Specifically, I investigate the utility of silk scaffolds as platforms for urogenital, gastrointestinal, and respiratory tissue reconstruction. By deciphering how variations in scaffold processing parameters can affect the in vivo performance of silk biomaterials, we seek to develop 3-D matrix designs with optimal structural, mechanical, and degradation profiles capable of restoring function in defects associated with traumatic injury and fibrotic diseases. Both small and large animal models of tissue repair in the bladder, urethra, small intestine, trachea and the esophagus are employed. In addition, my laboratory explores the mechanisms of constructive remodeling of tissue engineered constructs by delineating how host tissue microenvironments interact, populate, and integrate into scaffold environments in vivo. Our overall goal is the creation of clinically-useful scaffold configurations for hollow organ regeneration by engineering materials which fulfill structural and mechanical requirements of native tissues as well as present microenvironmental cues necessary for host tissue integration and defect consolidation. My comprehensive background in silk-based biomaterial fabrication methods coupled with my expertise in stem cell biology uniquely positions my lab as a pioneer in the creation of novel strategies for hollow organ regeneration. In addition, I have been continuously NIH-funded since 2011.

2020 Faculty Excellence in Research-Mid-Career Award for SoE University of California, Irvine

Joshua Mauney is a tenured Associate Professor in the Departments of Urology and Biomedical Engineering at the University of California, Irvine. He holds the Jerry D. Choate Presidential Endowed Chair in Urology Tissue Engineering. He received his BSc in Chemical Engineering and PhD in Biotechnology Engineering from Tufts University. Dr. Mauney’s laboratory focuses on the development silk fibroin graft for the repair of visceral hollow organs including the bladder, urethra, and esophagus. He also specializes in the creation of novel large animal models of urinary tract and gastrointestinal disease for preclinical medical device testing.

Gundogdu G, Morhardt D, Cristofaro V, Algarrahi K, Yang X, Costa K, Galvez Alegria C, Sullivan M, Mauney JR. Evaluation of Bi-layer Silk Fibroin Grafts for Tubular Esophagoplasty in a Porcine Defect Model. Tissue Engineering, Part A. 2020 In Press.

Affas S, Schäfer FM, Algarrahi K, Cristofaro V, Sullivan MP, Yang X, Costa K, Sack BS, Gharaee-Kermani M, Macoska JA, Gundogdu G, Seager C, Estrada CR, Mauney JR. Augmentation Cystoplasty of Diseased Porcine Bladders with Bi-Layer Silk Fibroin Grafts. Tissue Engineering, Part A. 2019. In Press. PMID: 30191762

Galvez Alegria C, Gundogdu G Yang X, Costa K, Mauney JR. Evaluation of Acellular Bi-Layer Silk Fibroin Grafts for Onlay Tracheoplasty in a Rat Defect Model. Otolaryngology - Head and Neck Surgery. 2019 Feb;160(2):310-319. PMID: 30274546

Algarrahi K, Affas S, Sack BS, Yang X, Costa K, Seager C, Estrada CR Jr, Mauney JR. Repair of Injured Urethras with Silk Fibroin Scaffolds in a Rabbit Model of Onlay Urethroplasty. Journal of Surgical Research. J Surg Res. 2018 Sep;229:192-199. PMID: 29936989

Algarrahi K, Franck D, Savarino A, Cristofaro V, Yang X, Affas S, Schäfer FM, Sullivan MP, Estrada CR Jr, Mauney JR. Bi-layer Silk Fibroin Grafts Support Functional Esophageal Repair in a Rodent Model of Caustic Injury. J Tissue Eng Regen Med. 2018 Feb;12(2):e1068-e1075. PMID: 28371514

Algarrahi K, Franck D, Cristofaro V, Yang X, Savarino A, Affas S, Schäfer FM, Ghezzi C, Jennings R, Nedder A, Kaplan DL, Sullivan MP, Estrada CR Jr, Mauney JR. Bi-layer Silk Fibroin Grafts Support Functional Tissue Regeneration in a Porcine Model of Onlay Esophagoplasty. J Tissue Eng Regen Med. 2018 Feb;12(2):e894-e904. PMID: 28084044

Schäfer FM, Algarrahi K, Savarino A, Yang X, Seager C, Franck D, Costa K, Liu S, Logvinenko T, Adam R, Mauney JR. Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair. Stem Cell Reports. 2017 Dec 12;9(6):2005-2017. PMID: 29173895

Chung YG, Seth A, Doyle C, Franck D, Kim D, Cristofaro V, Benowitz LI, Tu DD, Estrada CR, Mauney JR, Sullivan MP, Adam RM. Inosine Improves Neurogenic Detrusor Overactivity Following Spinal Cord Injury. PLoS One. 2015 Nov 3;10(11):e0141492. PMID: 26529505

Algarrahi K, Franck D, Ghezzi C, Cristafaro V, Yang X, Sullivan MP, Chung YG, Affas S, Jennings R, Kaplan DL, Estrada CR, Mauney JR. Acellular Bi-Layer Silk Fibroin Scaffolds Support Functional Tissue Regeneration in a Rat Model of Onlay Esophagoplasty. Biomaterials. 2015 Jun;53:149-59.2015. PMID: 25890715

Franck D, Chung YG, Coburn J, Kaplan DL, Estrada CR Jr, Mauney JR. In vitro evaluation of bi-layer silk fibroin scaffolds for gastrointestinal tissue engineering. J Tissue Eng. 2014 Nov 5;5:2041731414556849. PMID: 25396043.

Chung YG, Algarrahi K, Franck D, Tu DD, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The Use of Bi-layer Silk Fibroin Scaffolds and Small Intestinal Submucosa Matrices to Support Bladder Tissue Regeneration in a Rat Model of Spinal Cord Injury. Biomaterials. 2014 Aug;35(26):7452-9. PMID: 24917031.

Chung YG, Tu D, Franck D, Gil ES, Algarrahi K, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS One. 2014 Mar 14;9(3):e91592. PMID: 24632740.

Gandhi D, Molotkov A, Batourina E, Schneider K, Dan H, Reiley M, Laufer E, Metzger D, Liang F, Liao Y, Sun TT, Aronow B, Rosen R, Mauney J, Adam R, Rosselot C, Van Batavia J, McMahon A, McMahon J, Guo JJ, Mendelsohn C. Retinoid signaling in progenitors controls specification and regeneration of the urothelium. Dev Cell. 2013 Sep 16;26(5):469-82. PMID: 23993789.

Tu DD, Chung YG, Gil ES, Seth A, Franck D, Cristofaro V, Sullivan MP, Di Vizio D, Gomez P 3rd, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty. Biomaterials. 2013 Nov;34(34):8681-9. PMID: 23953839.

Seth A, Chung YG, Gil ES, Tu D, Franck D, Di Vizio D, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The performance of silk scaffold in a rat model of augmentation cystoplasty. Biomaterials. 2013 Jul;34(20):4758-65. PMID: 23545287.

Franck D, Gil ES, Adam RM, Kaplan DL, Chung YG, Estrada CR, Mauney JR. Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells. PLoS One. 2013;8(2):e56237.

Tu D, Seth A, Gil ES, Kaplan DL, Estrada CR, Mauney JR. Evaluation of biomaterials for bladder augmentation using cystometric analyses in various rodent models. J. Vis. Exp. 2011. Aug 9;(66). doi:pii: 3981. 10.3791/3981. PMID: 22907252.

Gomez P, Gil ES, Lovett ML, Rockwood DN, Di Vizio D, Kaplan DL, Adam RM, Estrada CR, Mauney JR. The effect of manipulation of silk scaffold fabrication parameters on matrix performance in a murine model of bladder augmentation. Biomaterials. 2011. Oct;32(30):7562-70. PMID: 21764119

Kim J, Mihee J, DiDonato J, Rackley R, Kuang M, Provash S, Mauney JR, Keay S, Freeman MR, Liou L, Adam RM. An hTERT-Immortalized Human Urothelial Cell Line that Responds to Anti-Proliferative Factor. In Vitro Cell Dev Biol Anim. 2011 Jan;47(1):2-9. PMID: 21136194

Mauney JR, Cannon GM, Lovett ML, Gong EM, Di Vizio D, Gomez P 3rd, Kaplan DL, Adam RM, Estrada CR Jr. Evaluation of gel spun silk-based biomaterials in a murine model of bladder augmentation. Biomaterials. 2011 Jan;32(3):808-18. PMID: 20951426

Mauney J, Olsen BR, Volloch V. Matrix remodeling and stem cell recruitment: A novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol. 2010 Oct;29(8):657-63. PMID: 20828613

Mauney JR, Ramachandran A, Yu RN, Daley GQ, Adam RM, Estrada CR. All-trans Retinoic acid Directs Urothelial Specification of Murine Embryonic Stem Cells via GATA4/6 Signaling Mechanisms. PLoS ONE. 2010 Jul 13;5(7):e11513. PMID: 20644631

Mauney J, Volloch V. Human bone marrow-derived stromal cells show highly efficient stress-resistant adipogenesis on denatured collagen IV matrix but not on its native counterpart: Implications for obesity. Matrix Biol. 2010 Jan;29(1):9-14. PMID: 19761844

Mauney J, Volloch V. Adult human bone marrow stromal cells regulate expression of their MMPs and TIMPs in differentiation type-specific manner. Matrix Biol. 2010 Jan;29(1):3-8. PMID: 19765656

Mauney J, Volloch V. Progression of human bone marrow stromal cells into both osteogenic and adipogenic lineages is differentially regulated by structural conformation of collagen I matrix via distinct signaling pathways. Matrix Biol. 2009 Jun;28(5):239-50. PMID: 19375503

Mauney J, Volloch V. Collagen I matrix contributes to determination of adult human stem cell lineage via differential, structural conformation-specific elicitation of cellular stress response. Matrix Biol. 2009 Jun;28(5):251-62. PMID: 19375506

Egles C, Shamis Y, Mauney JR, Volloch V, Kaplan DL, Garlick JA. Denatured Collagen Modulates the Phenotype of Normal and Wounded Human Skin Equivalents. J Invest Dermatol. 2008 Jul;128(7):1830-7. PMID: 18200055

Mauney JR, Nguyen T, Gillen KM, Kirker-Head C, Gimble JM, Kaplan DL. Engineering Adipose-like Tissue in vitro and in vivo Utilizing Human Bone Marrow and Adipose-derived Mesenchymal Stem Cells with Silk Fibroin 3D Scaffolds. Biomaterials. 2007 Dec;28(35):5280-90. PMID: 17765303

Moreau J, Anderson KM, Mauney JR, Kaplan DL, Rosenblatt M. Tissue Engineered Bone Serves as a Target for Metastasis of Human Breast Cancer in a Mouse Model. Cancer Res. 2007. Nov 1;67(21):10304-8. PMID: 17974972

Lu D, Mahmood A, Mauney JR, Qu C, Hong X, Kaplan D, Chopp M. Collagen scaffolds populated with human marrow stromal cells reduce lesion volume and improve functional outcome after traumatic brain injury. Neurosurgery. 2007 Sep;61(3):596-602. PMID: 17881974

Mauney JR, Kirker-Head C, Abrahamson L, Gronowicz G, Volloch V, Kaplan DL. Matrix-Mediated Retention of In vitro Osteogenic Differentiation Potential and In vivo Bone-Forming Capacity by Human Adult Bone Marrow-Derived Mesenchymal Stem Cells During Ex vivo Expansion. J Biomed Mater Res A. 2006 Dec 1;79(3):464-75. PMID: 16752403

Mauney JR, Kaplan DL, Volloch V. Matrix-mediated retention of adipogenic differentiation potential by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Biomaterials. 2005 Nov;26(31):6167-75. PMID: 15913765

Mauney JR, Jaquiery C, Volloch V, Heberer M., Martin I, Kaplan DL. In vitro and in vivo Evaluation of Differentially Demineralized Cancellous Bone Scaffolds Combined with Human Bone Marrow Stromal Cells for Tissue Engineering. Biomaterials. 2005 Jun;26(16):3173-85. PMID: 15603812

Mauney JR, Kaplan DL, Volloch V. Matrix-mediated Retention of Osteogenic Differentiation Potential by Human Adult Bone Marrow Stromal Cells During ex vivo Expansion. Biomaterials. Jul;25(16):3233-43. 2004. PMID: 14980418

Mauney JR, Sjostrom S. Blumberg J, Horan RL, O`Leary J, Volloch V, Vunjak-Novakovic G, Kaplan DL. Mechanical Stimulation Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells on 3-D Partially Demineralized Bone Scaffolds In Vitro. Calcified Tissue International. May;74(5):458-68. Epub 2004 Feb 17. 2004. PMID: 14961210



Mauney JR, Blumberg J, Pirun M, Volloch V, Vunjak-Novakovic G, Kaplan DL. Osteogenic Differentiation of Human Bone Marrow Stromal Cells on Partially Demineralized Bone Scaffolds in vitro. Tissue Eng. Jan-Feb;10(1-2):81-92. 2004. PMID: 15009933

Sack B, Mauney JR, Estrada CR. Silk fibroin scaffolds for urologic tissue engineering. Current Urology Reports. Curr Urol Rep. 2016 Feb;17(2):16. Review. PMID: 26801192.

Mauney JR, Adam RM. Dynamic reciprocity in cell-scaffold interactions. Adv Drug Deliv Rev. 2015 Mar;82-83:77-85. Review. PMID: 25453262.

DeGraff DJ, Cates JM, Mauney JR, Clark PE, Matusik RJ, Adam RM. When Urothelial Differentiation Pathways Go Wrong: Implications for Bladder Cancer. Urologic Oncology: Seminars and Original Investigations. Urol Oncol. 2013 Aug;31(6):802-11. Review. PMID: 21924649.

Moreau J, Anderson KM, Mauney JR, Kaplan DL, Rosenblatt M. Studies of osteotropism on both sides of the breast cancer-bone section. Ann N Y Acad Sci. 2007 Nov;1117:328-44. Review. PMID: 17584985.

Mauney JR, Volloch V, Kaplan DL. Role of adult mesenchymal stem cells in bone tissue engineering applications: current status and future prospects. Tissue Eng. 2005 May-Jun;11(5-6):787-802. Review. PMID: 15998219.

R01DK119240 (MAUNEY, JOSHUA ROBERT) Dec 18, 2019 - Apr 30, 2024 NIH/NIDDK Partial Bladder Outlet Obstruction: Mechanisms of Injury and Novel Repair Strategies Role: Principal Investigator R01DK107568 (MAUNEY, JOSHUA ROBERT) Oct 12, 2019 - Jun 30, 2021 NIH/NIDDK Development of Silk Fibroin Grafts for Reconstruction of Esophageal Defects Role: Co-Principal Investigator R21EB021482 (MAUNEY, JOSHUA ROBERT) Mar 3, 2016 - Dec 31, 2018 NIH/NIBIB Instructive Biomaterial Designs for Urethroplasty Role: Co-Principal Investigator R21EB020860 (MAUNEY, JOSHUA ROBERT) Jul 6, 2015 - Jun 30, 2017 NIH/NIBIB Development of Silk Fibroin Grafts for Reconstruction of Pathological Bladders Role: Co-Principal Investigator R00DK083616 (MAUNEY, JOSHUA ROBERT) Sep 1, 2012 - Jul 31, 2015 NIH/NIDDK Novel Strategies in Bladder Tissue Engineering: Stem Cells and Silk Biomaterials Role: Principal Investigator

Biomedical Engineering

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Last updated
01/29/2021