Nick R. Pannunzio PhD

picture of Nick R. Pannunzio

Assistant Professor, Division of Hematology/Oncology, Medicine
School of Medicine

Ph.D., Beckman Research Institute of City of Hope, 2010, Genetics and Molecular Biology
B.S., University of Pittsburgh, 2002, Microbiology

Phone: (949) 824-5638

University of California, Irvine
839 Health Sciences Road
Sprague Hall, Room 124
Mail Code: 3905
Irvine, CA 92697
Research Interests
cancer genetics, B cell malignancies, chromosomal translocations, DNA damage and repair, cancer health disparities
Research Abstract
Chromosomal translocations are a recurrent, transformative feature of many cancers and occur following formation of DNA double-strand breaks (DSBs). While critical to development of antibody producing B cells, improper repair of DSBs is a major factor in the etiology of B cell malignancies. Using genetic and pharmacological approaches, the Pannunzio lab seeks to understand how internal factors (torsional stress, transcription, replication, DNA modifying enzymes) and external factors (reactive oxygen species, radiation, chemotherapy drugs, environmental toxins) contribute to DSB formation. Using the latest technology, we quantify DSBs in human B cells carrying mutations in disease relevant genes or exposed to DNA damaging agents.

Another major component of our research is understanding cancer health disparities present in certain races and ethnicities. Our work seeks to define what genetic, epigenetic, and environmental factors make Hispanics, Latinos, and Native Americans disproportionately affected by certain subtypes of acute lymphoblastic leukemia and why the incidence rate of multiple myeloma is higher in African Americans.
Awards and Honors
2022 NIH/NCI R37 MERIT Award
2019 USC Department of Pathology Seminar of the Year Award
2018 FASEB Travel Award
2017 USC Department of Pathology Paper of the Year Award
2010-2015 ARCS Foundation, Los Angeles Founder Chapter, John H. Richardson
Postdoctoral Fellowship
2008 Rachmiel Levine Student Scientific Communication Award
2006 City of Hope Graduate Education Travel Award
2006-2008 American Heart Association Pre-Doctoral Fellowship
2002 Graduated University of Pittsburgh cum laude
Pedulla ML, Ford ME, Houtz JM, Karthikeyan T, Wadsworth C, Lewis JA, Jacobs-Sera D, Falbo J, Gross J, Pannunzio NR, Brucker W, Kumar V, Kandasamy J, Keenan L, Bardarov S, Kriakov J, Lawrence JG, Jacobs WR Jr, Hendrix RW, Hatfull GF. (2003) Origins of Highly Mosaic Mycobacteriophage Genomes. Cell, 113(2): 171-82. (cover article)

Ghosh P, Pannunzio NR, Hatfull GF. (2005). Synapsis of phage Bxb1 integration: selection mechanism for the correct pair of recombination sites. J. Mol. Biol. 349(2): 331-48.

Pannunzio NR, Manthey GM, Bailis AM. (2008). Rad59 is required for the efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae. DNA repair (Amst), 7(5): 788-800.

Pannunzio NR, Manthey GM, Bailis AM. (2010). RAD59 and RAD1 cooperate in translocation formation by single-strand annealing in Saccharomyces cerevisiae. Curr Genet, 56(1): 87-100.

Liddell L, Manthey G, Pannunzio N, Bailis A. (2011). Quantitation and analysis of the formation of HO-endonuclease stimulated chromosomal translocations by single-strand annealing in Saccharomyces cerevisiae. J Vis Exp, 55: 3150.

Pannunzio NR, Manthey GM, Liddell LC, Fu BXH, Roberts CM, Bailis AM. (2012). Rad59 regulates association of Rad52 with DNA double-strand breaks. MicrobiologyOpen. Sep;1(3);285-97.

Pannunzio NR. Li S, Watanabe G, Lieber MR. (2014). Non-homologous end joining often uses microhomology: implications for alternative end joining. DNA Repair (Amst). May;17:74-80.

Zhang ZZ, Pannunzio NR, Han L, Hsieh CL, Yu K, Lieber MR. (2014). The strength of an Ig switch region is determined by its ability to drive R look formation and its number of WGCW sites. Cell Rep. Jul 24;8(2):557-69.

Zhang ZZ, Pannunzio NR, Hsieh CL, Yu K, Lieber MR. (2014). The role of G-density in switch region repeats for immunoglobulin class switch recombination. Nucleic Acids Res. Dec 1;42(21):13186-93.

Zhang ZZ, Pannunzio NR, Hsieh CL, Yu K, Lieber MR. (2015). Complexities due to single-stranded RNA during antibody detection of genomic RNA:DNA hybrids. BMC Res Notes. Apr 8,8:127.

Zhang ZZ, Pannunzio NR, Lu Z, Hsu E, Yu K, Lieber MR. (2015). The repetitive portion of the Xenopus IgH mu switch region mediates orientation-dependent class switch recombination. Molecular Immunology. Oct; 67(2PtB):524-31.

Lu Z, Pannunzio NR, Greisman H, Cesaro D, Parekh C, Lieber MR. (2015). Convergent BCL6 and lncRNA promoters demarcate the major breakpoint region for BCL6 translocations. Blood. Oct 1; 126(14):1730-1

Pannunzio NR, Lieber MR. (2016). Dissecting the roles of divergent and convergent transcription in chromosome instability. Cell Reports. Feb 9; 14(5):1025-31.

Pannunzio NR, Lieber MR. (2016). RNA polymerase collision versus DNA structural distortion: twists and turns can cause break failure. Molecular Cell. May 5; 62(3):327-34.

Chang HHY, Pannunzio NR, Adachi N, Lieber MR. (2017) Non-homologous DNA end joining and alternative pathways to double-strand break repair. Nat Rev Mol Cell Biol. May 17. (cover article)

Pannunzio NR, Lieber MR. (2017) AID and reactive oxygen species can induce DNA breaks within human chromosomal translocation fragile zones. Molecular Cell. December 7; 68:901-912. (cover article)

Pannunzio NR, Watanabe G, Lieber MR. (2018) Nonhomologous DNA end-joining for repair of DNA double-strand breaks. J Biol Chem 293:10512-10523. (cover article)

Pannunzio NR, Lieber MR. (2018) Concept of DNA Lesion Longevity and Chromosomal Translocations. Trends Biochem Sci 43:490-498. (cover article)

Pannunzio NR, Lieber MR. (2019) Constitutively Active Artemis Nuclease Recognizes Structures Containing Single-Stranded DNA Configurations. DNA Repair.

Sterrenberg, JN, Folkerts ML, Rangel V, Lee SE, Pannunzio NR. (2022) Diversity Upon Diversity: Linking DNA Double-Strand Break Repair to Blood Cancer Health Disparities. Trends in Cancer.
Graduate Programs
Cellular and Molecular Biosciences

Research Centers
Chao Family Comprehensive Cancer Center
Center for Epigenetics and Metabolism
Institute for Immunology
Cancer Research Institute
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