Philip D Kiser

picture of Philip D Kiser

Assistant Professor, Physiology & Biophysics
School of Medicine

Assistant Professor, Ophthalmology
School of Medicine

Assistant Professor, Department of Clinical Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences,
College of Health Sciences

Ph.D., Case Western Reserve University, 2010, Pharmacology
Pharm.D., St. Louis College of Pharmacy, 2005, Pharmacy

Phone: 949-824-6954
Fax: 949-824-8540

University of California, Irvine
Gillespie Neuroscience Research Facility
RM 2216
837 Health Sciences Rd
Irvine, CA 92617
Research Interests
Vision Science, retinoid biochemistry, carotenoid oxygenases, pharmacology
Research Abstract
My laboratory studies the enzymes, transporters and binding proteins that comprise a metabolic pathway known as the visual cycle as well as a variety of other proteins important for the health of the retina. We use an array of experimental techniques and methodologies, including X-ray crystallography, electron microscopy, knockout mouse models, and classic biochemistry approaches to answer important questions about the structure and function of these proteins. Major emphasis is placed on the use of such information to develop effective therapeutics for currently untreatable retinal diseases.
Awards and Honors
Pisart Award in Vision Science, Lighthouse Guild, 2020

March of Dimes Travel Award, FASEB Biology and Chemistry of Vision Conference, 2015

NIH Pediatric Research Loan Repayment Program Award, 2006-2010

Doctoral Excellence Award in Pharmacology, Case Western Reserve University, 2010

Merck Award for Highest Rank Collectively in Pharmacology, Pharmacy Practice, Therapeutics, and Medicinal Chemistry, St. Louis College of Pharmacy, 2004
Occelli, L. M., Daruwalla, A., De Silva, S. R., Winkler, P. A., Sun, K., Pasmanter, N., Minella, A., Querubin, J., Lyons, L. A., Consortium, L., Robson, A. G., Heon, E., Michaelides, M., Webster, A. R., Palczewski, K., Vincent, A., Mahroo, O. A., Kiser, P. D., and Petersen-Jones, S. M. (2021) A large animal model of RDH5-associated retinopathy recapitulates important features of the human phenotype. Hum Mol Genet, accepted
Sander, C. L., Luu, J., Kim, K., Furkert, D., Jang, K., Reichenwallner, J., Kang, M., Lee, H-J., Eger, B. T., Choe, H-W., Fiedler, D., Ernst, O. P., Kim, Y. J., Palczewski, K., and Kiser, P. D.# (2021) Structural evidence for visual arrestin priming via complexation of phosphoinositols. Structure, accepted
Sander, C. L., Sears, A. E., Pinto, A. F. M., Choi, E. H., Kahremany, S., Gao, F., Salom, D., Jin, H., Pardon, E., Suh, S., Dong, Z., Steyaert, J., Saghatelian, A., Skowronska-Krawczyk, D.#, Kiser, P. D.#, and Palczewski, K.# (2021) Nano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition. J. Cell Biol. 220
Choi, E. H., Suh, S., Einstein, D. E., Leinonen, H., Dong, Z., Rao, S. R., Fliesler, S. J., Blackshaw, S., Yu, M., Peachey, N. S., Palczewski, K., and Kiser, P. D.# (2021) An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight 6
Blum, E., Zhang, J., Zaluski, J., Einstein, D. E., Korshin, E., Kubas, A., Gruzman, A.#, Tochtrop, G. P.#, Kiser, P. D.#, and Palczewski, K.# (2021) Rational alteration of pharmacokinetics of chiral fluorinated and deuterated derivatives of emixustat for retina therapies. J. Med. Chem. 64, 8287-8302
York, N. J., Lockart, M. M., Sardar, S., Khadka, N., Shi, W., Stenkamp, R. E., Zhang, J., Kiser, P. D.#, and Pierce, B. S.# (2021) Structure of 3-mercaptopropionic acid dioxygenase with a substrate analog reveals bidentate substrate binding at the iron center. J. Biol. Chem. 100492
Kolesnikov, A. V., Kiser, P. D., Palczewski, K., and Kefalov, V. J. (2021) Function of mammalian M-cones depends on the level of CRALBP in Muller cells. J. Gen. Physiol. 153
Kiser, P.D., and Palczewski, K. (2021) Pathways and disease-causing alterations in visual chromophore production for vertebrate vision. J. Biol. Chem. 296
Daruwalla, A., Zhang, J., Lee, H. J., Khadka, N., Farquhar, E. R., Shi, W., von Lintig, J., and Kiser, P. D.# (2020) Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase. Proc Natl Acad Sci U S A 117, 19914-19925
Suh, S., Choi, E. H., Leinonen, H., Foik, A. T., Newby, G. A., Yeh, W. H., Dong, Z., Kiser, P. D., Lyon, D. C., Liu, D. R., and Palczewski, K. (2020) Restoration of visual function in adult mice with an inherited retinal disease via adenine base editing. Nat Biomed Eng 5, 169-178
Gazda, M. A., Toomey, M. B., Araujo, P. M., Lopes, R. J., Afonso, S., Myers, C. A., Serres, K., Kiser, P. D., Hill, G. E., Corbo, J. C., and Carneiro, M. (2020) Genetic Basis of De Novo Appearance of Carotenoid Ornamentation in Bare-Parts of Canaries. Mol. Biol. Evol. 37, 1317-1328
Kiser, P.D. (2019) Alkene-cleaving carotenoid cleavage dioxygenases. in Encyclopedia of Inorganic and Bioinorganic Chemistry (Scott, R. A. ed.), John Wiley & Sons, Ltd. 1-13
Kiser, P. D.#, Kolesnikov, A. V., Kiser, J. Z., Dong, Z., Chaurasia, B., Wang, L., Summers, S. A., Hoang, T., Blackshaw, S., Peachey, N. S., Kefalov, V. J., and Palczewski, K. (2019) Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. FASEB J. 33, fj201802493R
Khadka, N., Farquhar, E. R., Hill, H. E., Shi, W., von Lintig, J., and Kiser, P. D.# (2019) Evidence for distinct rate-limiting steps in the cleavage of alkenes by carotenoid cleavage dioxygenases. J. Biol. Chem. 294, 10596-10606
Sui, X., Farquhar, E. R., Hill, H. E., von Lintig, J., Shi, W., and Kiser, P. D.#(2018) Preparation and characterization of metal-substituted carotenoid cleavage oxygenases. Journal of Biological Inorganic Chemistry 23, 887-901
Choi, E. H., Suh, S., Sander, C. L., Hernandez, C. J. O., Bulman, E. R., Khadka, N., Dong, Z., Shi, W., Palczewski, K.#, and Kiser, P. D.# (2018) Insights into the pathogenesis of dominant retinitis pigmentosa associated with a D477G mutation in RPE65. Human Molecular Genetics 27, 2225-2243
Kiser, P. D.#, Zhang, J., Sharma, A., Angueyra, J. M., Kolesnikov, A. V., Badiee, M., Tochtrop, G. P., Kinoshita, J., Peachey, N. S., Li, W., Kefalov, V. J.#, and Palczewski, K.# (2018) Retinoid isomerase inhibitors impair but do not block mammalian cone photoreceptor function. Journal of General Physiology 150, 571-590
Sui, X., Weitz, A. C., Farquhar, E. R., Badiee, M., Banerjee, S., von Lintig, J., Tochtrop, G. P., Palczewski, K., Hendrich, M. P.#, and Kiser, P. D.# (2017) Structure and Spectroscopy of Alkene-Cleaving Dioxygenases Containing an Atypically Coordinated Non-Heme Iron Center. Biochemistry 56, 2836-2852
Kiser, P. D., Zhang, J., Badiee, M., Kinoshita, J., Peachey, N. S., Tochtrop, G. P., and Palczewski, K. (2017) Rational Tuning of Visual Cycle Modulator Pharmacodynamics. Journal of Pharmacology and Experimental Therapeutics 362, 131-145
Sui, X., Zhang, J., Golczak, M., Palczewski, K., and Kiser, P. D. (2016) Key Residues for Catalytic Function and Metal Coordination in a Carotenoid Cleavage Dioxygenase. Journal of Biological Chemistry 291, 19401-19412
Kiser, P. D., Zhang, J., Badiee, M., Li, Q., Shi, W., Sui, X., Golczak, M., Tochtrop, G. P., and Palczewski, K. (2015) Catalytic mechanism of a retinoid isomerase essential for vertebrate vision. Nature Chemical Biology 11, 409-415
I01 BX004939-01 4/1/2020 – 3/31/2024 Department of Veterans Affairs Title: Modulation of retinoid reactivity and pathological signaling in retinal therapeutics Role: PI
R24 EY027283 4/1/2017 – 3/31/2022 National Eye Institute Title: Use of systems pharmacology to prevent rod and cone photoreceptor degeneration Role: Co-I (Module Director)
NSF-2107713 9/1/2021 – 8/31/2024 National Science Foundation Title: Unraveling the principles of catalytic diversity in the carotenoid oxygenase superfamily Role: PI
Other Experience
Research Health Scientist
VA Long Beach Healthcare System 2015—pres

Staff Pharmacist
Pharmacy Systems Inc. 2014—2018

Staff Pharmacist
CVS Pharmacy 2005—2008

Graduate Programs
Cellular and Molecular Biosciences

Interdepartmental Neuroscience Program

Structural Biology and Molecular Biophysics

Research Center
Center for Translational Vision Research
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