Medha M. Pathak

picture of Medha M. Pathak

Assistant Professor, Physiology & Biophysics
School of Medicine

Ph.D., UC Berkeley, 2006, Biophysics
M.S., National Centre for Biological Sciences, Bangalore, India, 2000, Neuroscience
B.S., St. Xavier's College, Ahmedabad, India, 1996, Biochemistry
Postdoc, Harvard Medical School, Neurobiology

Phone: (949) 824-6623
Fax: (949) 824-8540

University of California, Irvine
3026 Gross Hall (Office)
2200 Gross Hall (Lab)
Mail Code: 4560
Irvine, CA 92697
Research Interests
Piezo1, ion channels, stem cells, neural stem cells, differentiation, development, mechanical forces, matrix, environment
Academic Distinctions
Howard Hughes Medical Institution's Gilliam Fellowship for Advanced Study
NIH Director's New Innovator Award
UCI Chancellor’s Award for Excellence in Undergraduate Research
UCI ADVANCE Faculty Career Development Award
Junior Faculty Fellow, Journal of General Physiology
GSK Neuroscience Discovery Award, FASEB Ion Channel Regulation Conference
Helen Hay Whitney Postdoctoral Fellowship
Research Abstract
The long-term goal of my lab is to understand at molecular, cellular and organismal levels, how mechanical forces modulate neural stem cell fate in development and repair. We previously showed that the stretch-activated ion channel Piezo1 mediates mechanosensitive lineage specification of neural stem cells. Our studies revealed that Piezo1 activity in neural stem cells is modulated by matrix mechanics, and that it influences differentiation of the cells into neurons or astrocytes. Current work focuses on understanding (i) how Piezo1 detects and transduces matrix mechanical signals, (ii) how Piezo1 activity elicits gene expression changes, (iii) how Piezo1 may shape neural development, and (iv) how Piezo1 may be involved in certain disease states. We use a multi-disciplinary approach, combining ideas and techniques from ion channel biophysics, cell biology, optical imaging, stem cell biology and bioengineering.
Ellefsen K*, Chang A*, Holt JR*, Nourse JL*, Arulmoli A, Mekhdjian A, Abuwarda H, Tombola F, Flanagan LA, Dunn AR, Parker I, Pathak MM. (2019).
Myosin-II mediated traction forces evoke localized Piezo1 Ca2+ flickers.
Communications Biology. 2: 298.
Nourse JL, Pathak MM (2017).
How cells channels their stress: Interplay betwen Piezo1 and the Cyoskeleton
Seminars in Cell and Development Biology. 2017 Nov; 71:3-12.
Pathak MM, Tran T, Hong L, Joós B, Morris CE, Tombola F (2016).
The Hv1 proton channel responds to mechanical stimuli.
Journal of General Physiology. 2016 Nov;148(5):405-418.
Arulmoli J, Wright HJ, Phan DTT, Sheth U, Que RA, Botten GA, Keating M, Botvinick EL, Pathak MM, Zarembinski TI, Yanni DS, Razorenova OV, Hughes CCW, Flanagan LA (2016).
Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering.
Acta Biomaterialia 2016 Oct 1;43:122-38.
Phan L*, Kautz R*, Arulmoli J, Kim I, Le DT, Shenk MA, Pathak MM†, Flanagan LA†, Tombola F†, Gorodetsky AA† (2016).
Reflectin as a Material for Neural Stem Cell Growth.
ACS Applied Materials & Interfaces. 13;8(1):278-84
† Corresponding authors.

Arulmoli J, Pathak MM, McDonnell LP, Nourse JL, Tombola F, Earthman JC, Flanagan LA. (2015)
Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner.
Scientific Reports. 5: 8499.

Pathak MM†, Nourse JL, Tran T, Hwe J, Arulmoli J, Le DTT, Bernardis E, Flanagan LA, Tombola F†. (2014)
Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells.
Proceedings of the National Academy of Sciences. 111(45):16148-53.
† Corresponding authors.

Kim IH, Hevezi P, Varga C, Pathak MM, Hong L, Ta D, Tran CT, Zlotnik A, Soltesz I, Tombola F. (2014).
Evidence for functional diversity between the voltage-gated proton channel Hv1 and its closest related protein HVRP1.
PLoS One. 9(8):e105926.

Nourse JL*, Prieto JL*, Dickson AR, Lu J, Pathak MM, Tombola F, Demetriou M, Lee AP, Flanagan LA. (2014).
Membrane biophysics define neuron and astrocyte progenitors in the neural lineage.
Stem Cells. 32(3):706-16.

Hong L, Pathak MM, Kim IH, Ta D, Tombola F. (2013).
Voltage-sensing domain of voltage-gated proton channel Hv1 shares mechanism of block with pore domains.
Neuron. 77(2):274-87.
Commentary: Kalia & Schwartz (2013). Common principles of voltage-dependent gating for Hv and Kv channels. Neuron. 77(2):214-6.

Pathak MM*, Yarov-Yarovoy V*, Roux B, Agarwal G, Kohout S, Barth P, Tombola F, Isacoff EY. (2007).
Closing in on the resting state of the Shaker K+ channel.
Neuron. 56(1):124-40.

Tombola F, Pathak MM, Gorostiza P, Isacoff EY. (2007).
The twisted ion-permeation pathway of a resting voltage-sensing domain.
Nature. 445(7127):546-9.

Tombola F, Pathak MM, Isacoff EY. (2006).
How does voltage open an ion channel?
Annual Review of Cell and Developmental Biology. 22:23-52.

Tombola F, Pathak MM, Isacoff EY. (2005).
How far will you go to sense voltage?
Neuron. 48:719-25.

Tombola F, Pathak MM, Isacoff EY. (2005).
Voltage-sensing arginines in a potassium channel permeate and occlude cation-selective pores.
Neuron. 45:379-88.

Pathak MM, Kurtz L, Tombola F, Isacoff EY. (2005).
The cooperative voltage sensor motion that gates a potassium channel.
Journal of General Physiology.125:57-69.

* Denotes Equal Contribution
NIH New Investigator Award (DP2, Principal Investigator). Building the brain: How mechanical forces shape human neural development. $1,500,000. 9/30/2018 – 6/30/2023.
NIH R01 grant (Principal Investigator). Piezo1 in neural stem cell mechano-regulation. $1,117,990. 9/30/2018 – 6/30/2023.
HHMI Gilliam Fellowship for PI Advanced Studies (Principal Investigator). Functional dynamics of Piezo1 and Traction Forces in Tissue Repair. $150,000. 9/1/2019 – 8/30/2022.
Professional Societies
Biophysical Society
American Society for Cell Biology
International Society for Stem Cell Research
Biomedical Engineering Society
American Association for the Advancement of Science
Graduate Programs
Cellular and Molecular Biosciences

Interdepartmental Neuroscience Program

Mathematical and Computational Biology

Medical Scientist Training Program

Research Centers
Sue and Bill Gross Stem Cell Research Center
Center for Complex Biological Systems
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