Donald Joseph Brown

picture of Donald Joseph Brown

Assistant Professor, Ophthalmology
School of Medicine

Ph.D., University of Colorado at Denver, 1984, Pathology

Phone: (949) 824-8936
Fax: (949) 824-9626

University of California, Irvine
Gavin Herbert Eye Institute
843 Health Sciences Rd.
Rm. 2034
Mail Code: 4390
Irvine, CA 92697
Research Interests
Glaucoma, Optic nerve, non-linear optics
Academic Distinctions
Invited lecturer: Association for Research in Vision and Ophthalmology Congress, Fort Lauderdale, FL. "Quantitative Assessment of the Extracellular Matrix in the Optic Nerve Head" (May 7, 2009).
Article in Medical News today ( Main Category: Eye Health / Blindness
Article Date: 11 Feb 2009 - 4:00 PST
Awarded the 2009 Shaffer Prize for Innovative Research from the Glaucoma Research Foundation, January 28, 2009.
Group leader Optic nerve/NFL anatomy and imaging section: The Glaucoma Summit V. Yosemite, CA (January 23, 2009)
Invited lecturer: The Glaucoma Summit V. Yosemite, CA (January 23-25, 2009)
Invited lecturer: International Congress for Eye Research, Bejing, China (September, 2008) – “Application of Non-linear Optics and Two-photon Excited Fluorescence to Assess Dynamic Structural Changes in Optic Nerve Head.”
Invited lecturer: International Society of Imaging in the Eye, Fort Lauderdale, Florida (May, 2008) – “Application of non-linear optics and two photon excited fluorescence to assess dynamic structural changes in the optic nerve head.”
Mentor for Annie Lay who was named a UROP Fellow for the 2007-2008 academic year.
Invited lecturer: Discovery Eye Foundation (April, 2008) – “Application of non-linear optics and two photon excited fluorescence to assess dynamic structural changes in the optic nerve head.”
Invited lecturer: Devers Eye Institute, Portland, Oregon (March, 2008) – “Application of non-linear optics to visualize ocular structures.”
Invited lecturer: Glaucoma Think Tank, New York (October, 2007) – “Probing ocular structure with non-linear optics".
Invited lecturer: Aspects of Innovation CME course, Beckman Institute, Irvine,Ca (June, 2006) – “Dynamic Visualization of the Human Lamina Cribrosa with Femtosecond Laser Energy".
Featured article: “Application of second harmonic imaging microscopy to assess structural changes in optic nerve head structure ex vivo. Brown DJ, Morishige N, Neekhra A, Minckler DS, Jester JV.” in Virtual Journal of Ultrafast Science -- May 2007 Volume 6, Issue 5.
Featured article: “Application of second harmonic imaging microscopy to assess structural changes in optic nerve head structure ex vivo. Brown DJ, Morishige N, Neekhra A, Minckler DS, Jester JV.” in Virtual Journal of Biological Physics Research -- April 15, 2007 Volume 13, Issue 8.
Published invited review: “The Cascade Hypothesis of Keratoconus” MC Kenney and DJ Brown.” in Contact Lens & Anterior Eye (2003) Volume 26, 139-146
Nominated for the College of Medicine Research Associates Dean’s Junior Physician/Scientist Award, 2004.
9/84 to 7/86 Post-Doctoral Fellow, Dept. of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA
Research Abstract
Primary open angle glaucoma (POAG) represents a serious and growing health problem accounting for ~12% of global blindness. Studies have identified age, ancestral group, and intra-ocular pressure (IOP) as significant risk factors for the development and progression of POAG. Our long-range goal is to understand the relationship between these established risk factors and the responsive loss of retinal ganglia cells that characterizes this disease. Current wisdom proposes that the biomechanical properties of the optic nerve head (ONH) and lamina cribrosa (LC) play a critical role in defining the pathology of POAG. In our studies, we have identified that regions of the LC susceptible to glaucomatous change (inferior, superior) contain less collagen and show more pressure-induced deformation (more compliant) than other regions of the LC (nasal and temporal). Building on these findings, the objective of our laboratory is to define the dynamic response of the ONH to IOP and determine the 3 dimensional biomechanical properties of the ONH by microscopically reconstructing the structural components and biomechanical properties across the LC at a micron scale and relate these properties to POAG risk factors.
Hwang, Hosik & Mikula, Eric & Xie, Yilu & Brown, Donald & Jester, James. (2020). A novel transillumination meibography device for in vivo imaging of mouse meibomian glands. The Ocular Surface. 19. 10.1016/j.jtos.2020.08.012.
Bradford, Samantha & Mikula, Eric & Juhasz, Tibor & Brown, Donald & Jester, James. (2020). Nonlinear optical crosslinking (NLO CXL) for correcting refractive errors. Experimental Eye Research. 199. 108199. 10.1016/j.exer.2020.108199.
Bradford, Samantha & Mikula, Eric & Xie, Yilu & Juhasz, Tibor & Brown, Donald & Jester, James. (2020). Enhanced Transepithelial Riboflavin Delivery Using Femtosecond Laser-Machined Epithelial Microchannels. Translational Vision Science & Technology. 9. 1. 10.1167/tvst.9.6.1.
Kim, Sun & Rho, Chang & Kim, Jinseor & Xie, Yilu & Prince, Richard & Mustafa, Khawla & Potma, Eric & Brown, Donald & Jester, James. (2020). Eicosapentaenoic acid (EPA) activates PPAR? signaling leading to cell cycle exit, lipid accumulation, and autophagy in human meibomian gland epithelial cells (hMGEC). The Ocular Surface. 18. 10.1016/j.jtos.2020.04.012.
Bradford, Samantha & Mikula, Eric & Kim, Sun & Xie, Yilu & Juhasz, Tibor & Brown, Donald & Jester, James. (2019). Nonlinear Optical Corneal Crosslinking, Mechanical Stiffening, and Corneal Flattening Using Amplified Femtosecond Pulses. Translational Vision Science & Technology. 8. 35. 10.1167/tvst.8.6.35.
Kim, Sun & Brown, Donald & Jester, James. (2019). Transcriptome analysis after PPAR? activation in human meibomian gland epithelial cells (hMGEC). The Ocular Surface. 17. 10.1016/j.jtos.2019.02.003.
Paugh, Jerry & Alfonso Garcia, Alba & Nguyen, Andrew & Suhalim, Jeffrey & Farid, Marjan & Garg, Sumit & Tao, Jeremiah & Brown, Donald & Potma, Eric & Jester, James. (2018). Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy. The Ocular Surface. 17. 10.1016/j.jtos.2018.10.003.
Koudouna, Elena & Mikula, Eric & Brown, Donald & Young, Robert & Quantock, Andrew & Jester, James. (2018). Cell regulation of collagen fibril macrostructure during corneal morphogenesis. Acta Biomaterialia. 79. 10.1016/j.actbio.2018.08.017.
Bradford, Samantha & Mikula, Eric & Juhasz, Tibor & Brown, Donald & Jester, James. (2018). Collagen fiber crimping following in vivo UVA-induced corneal crosslinking. Experimental Eye Research. 177. 10.1016/j.exer.2018.08.009.
Hwang, Hosik & Xie, Yilu & Koudouna, Elena & Kyung-Sun, Na & Young-Sik, Yoo & Suk-Woo, Yang & Brown, Donald & Jester, James. (2018). Light transmission/absorption characteristics of the meibomian gland. The Ocular Surface. 16. 10.1016/j.jtos.2018.07.001.
Kim, Sun & Xie, Yilu & Nguyen, Paul & Bui, Vickie & Huynh, Kelly & Kang, Jonathan & Brown, Donald & Jester, James. (2018). PPAR? regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC). The Ocular Surface. 16. 10.1016/j.jtos.2018.07.004.
Winkler, Moritz & Simon, Melinda & Vu, Timothy & Gartner, Trevor & Jester, James & Lee, Abraham & Brown, Donald. (2014). A microfabricated, optically accessible device to study the effects of mechanical cues on collagen fiber organization. Biomedical microdevices. 16. 10.1007/s10544-013-9829-x.
Sigal, Ian & Grimm, Jonathan & Jan, Ning-Jiun & Reid, Korey & Minckler, Don & Brown, Donald. (2013). Eye-Specific IOP-Induced Displacements and Deformations of Human Lamina Cribrosa. Investigative ophthalmology & visual science. 55. 10.1167/iovs.13-12724.
Parfitt, Geraint & Xie, Yilu & Geyfman, Mikhail & Brown, Donald & Jester, James. (2013). Absence of ductal hyper-keratinization in Mouse age-related meibomian gland dysfunction (ARMGD). Aging. 5. 10.18632/aging.100615.
Chai, Dongyul & Juhasz, Tibor & Brown, Donald & Jester, James. (2013). Nonlinear optical collagen cross-linking and mechanical stiffening: A possible photodynamic therapeutic approach to treating corneal ectasia. Journal of biomedical optics. 18. 38003. 10.1117/1.JBO.18.3.038003.
Suhalim, Jeffrey & Parfitt, Geraint & Xie, Yilu & De Paiva, Cintia & Pflugfelder, Stephen & Shah, Tejas & Potma, Eric & Brown, Donald & Jester, James. (2013). Effect of Desiccating Stress on Mouse Meibomian Gland Function. The Ocular Surface. 12. 10.1016/j.jtos.2013.08.002.
Jester JV, Brown DJ. Wakayama Symposium: Peroxisome Proliferator-Activated Receptor-Gamma (PPAR?) and Meibomian Gland Dysfunction. Ocul Surf. 2012Oct;10(4):224-9. doi: 10.1016/j.jtos.2012.07.001. Epub 2012 Jul 25. PubMed PMID: 23084144; PubMed Central PMCID: PMC3479436.
Parfitt GJ, Xie Y, Reid KM, Dervillez X, Brown DJ, Jester JV. A novel immunofluorescent computed tomography (ICT) method to localise and quantify multiple antigens in large tissue volumes at high resolution. PLoS One.2012;7(12):e53245. doi: 10.1371/journal.pone.0053245. Epub 2012 Dec 31. PubMedPMID: 23300899; PubMed Central PMCID: PMC3534019.
Chai D, Juhasz T, Brown DJ, Jester JV. Nonlinear optical collagencross-linking and mechanical stiffening: a possible photodynamic therapeuticapproach to treating corneal ectasia. J Biomed Opt. 2013 Mar;18(3):038003. doi:10.1117/1.JBO.18.3.038003. PubMed PMID: 23515869; PubMed Central PMCID:PMC3603223.
Chai D, Gaster RN, Roizenblatt R, Juhasz T, Brown DJ, Jester JV. Quantitative assessment of UVA-riboflavin corneal cross-linking using nonlinear optical microscopy. Invest Ophthalmol Vis Sci. 2011 Jun 16;52(7):4231-8. Print 2011 Jun. PubMed PMID: 21508101; PubMed Central PMCID: PMC3175975.
Winkler M, Chai D, Kriling S, Nien CJ, Brown DJ, Jester B, Juhasz T, Jester JV. Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics. Invest Ophthalmol Vis Sci. 2011 Nov 11;52(12):8818-27. Print 2011. PubMed PMID: 22003117; PubMed Central PMCID: PMC3230904.
Myrna KE, Mendonsa R, Russell P, Pot SA, Liliensiek SJ, Jester JV, Nealey PF, Brown D, Murphy CJ. Substratum topography modulates corneal fibroblast to myofibroblast transformation. Invest Ophthalmol Vis Sci. 2012 Feb 21;53(2):811-6. Print 2012. PubMed PMID: 22232431; PubMed Central PMCID: PMC3317421.
Jester JV, Brown D, Pappa A, Vasiliou V. Myofibroblast differentiation modulates keratocyte crystallin protein expression, concentration, and cellular light scattering. Invest Ophthalmol Vis Sci. 2012 Feb 16;53(2):770-8. Print 2012 Feb. PubMed PMID: 22247459; PubMed Central PMCID: PMC3317419.
Jester JV, Nien CJ, Vasiliou V, Brown DJ. Quiescent keratocytes fail to repair MMC induced DNA damage leading to the long-term inhibition of myofibroblast differentiation and wound healing. Mol Vis. 2012;18:1828-39. Epub 2012 Jul 4.PubMed PMID: 22815636; PubMed Central PMCID: PMC3398499.
Nien CJ, Flynn KJ, Chang M, Brown DJ, Jester JV. (2011). Reducing peak corneal haze after photorefractive keratectomy in rabbits: prednisolone acetate 1.00% versus cyclosporine A 0.05%. J Cataract Refract Surg. 2011 May;37(5):937-44.
Nien CJ, Massei S, Lin G, Nabavi C, Tao J, Brown DJ, Paugh JR, Jester JV. (2011). Effects of age and dysfunction on human meibomian glands. Arch Ophthalmol. 2011 Apr;129(4):462-9.
Chai D, Gaster RN, Roizenblatt R, Juhasz T, Brown DJ, Jester JV (2011). Quantitative Assessment of UVA-Riboflavin Corneal Cross-Linking using Non-Linear Optical Microscopy. Invest Ophthalmol Vis Sci. Apr 20. [Epub ahead of print].
Jester BE, Nien CJ, Winkler M, Brown DJ, Jester JV (2010). Volumetric reconstruction of the mouse Meibomian gland using high-resolution nonlinear optical imaging. Anatomic Record (Hoboken) Epub ahead of print. PMID: 21082731. Cover art
Jester JV, Winkler M, Jester BE, Nien C, Chai D, and Brown DJ. (2010) Evaluating corneal collagen organization using high-resolution nonlinear optical macroscopy. Eye & Contact Lens. 36(5):260-264.
Nien CJ, Massei S, Lin G, Liu G, Paugh J, Liu CJ, Kao W, Brown DJ, and Jester JV. (2010) The development of meibomian glands in mice. Molecular Vision 16:1132-1140.
Hao M, Flynn K, Nien CJ, Jester BE; Winkler M; Brown DJ, La Schiazza O, Bille J, and Jester JV. (2010) In Vivo Non Linear (NLO) imaging in live rabbit eyes using the Heidelberg Two-Photon Laser Ophthalmoscope. Exp. Eye Res. 91:308-314.
Gramajo AL, Zacharias LC, Neekhra A, Luthra S, Atilano SR, Chwa M, Brown DJ, Kuppermann BD, Kenney MC. (2010). Mitochondrial DNA damage induced by 7-ketocholesterol in human retinal pigment epithelial cells in vitro. Invest Ophthalmol Vis Sci. 51:1164-70.
Winkler M, Jester B, Nien-Shy CJ, Massei S, Minkler DS, Jester JV, and Brown DJ. (2010). High resolution three dimensional reconstruction of the collagenous matrix of the human optic nerve head. Brain Research Bulletin. 81:339-348.
Witt, D.P., D.J. Brown and J.A. Gordon: (1983) Transformation sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus. J. Cell Biol. 96:1766-1771.

Brown, D.J. and J.A. Gordon: (1984) The stimulation of pp60v-src activity by vanadate accompanies a new phosphorylation state of the enzyme. J. Biol. Chem. 259:9580-9586.

Nakajima, S., D.J. Brown, M. Ueda, K. Nakajima, A. Sugiura, A.K. Pattnaik and D.P. Nayak: (1986) Identification of the defects in the hemagglutinin gene of two temperature sensitive mutants of A/WSN/33 influenza virus. Virology, 154:85-96.

Pattnaik, A.K., D.J. Brown, and D.P. Nayak: (1986) Formation of influenza particles lacking hemagglutinin on the virus envelope. J. Virology, 60:994-1001.

Brown, D.J., and T.J. Bos: (1986) A modification for "smileless" polyacrylamide gels in nucleic acid sequencing. Electrophoresis 7:54-55.

Markushin, S., H. Ghiasi, N. Sokolov, A. Shilov, B. Sinitsin, D.J. Brown and D.P. Nayak: (1988) Nucleotide sequence of RNA segment 7 and the predicted amino acid sequence of M1 and M2 proteins of FPV/Weybridge (H7N7) and WSN (H1N1) influenza viruses: Specific amino acids correlate with host range of influenza viruses. Virus Res. 10:263-272.

Brown, D.J., B.G. Hogue and D.P. Nayak: (1988) Redundancy of signal and anchor functions in the NH2 terminal region of influenza virus neuraminidase, a class II membrane glycoprotein. J. Virology, 62:3824-3831.

Kenney, M.C., M. Chwa, M. Escobar and D.J. Brown: (1989) Altered gelatinolytic activity by keratoconus corneal cells. Biochem. Biophys. Res. Commun. 161:353-357.

Brown, D.J. and M.C. Kenney: (1989) High resolution 2 dimensional mapping of protease activities. J. Applied and Theoretical Electrophoresis, 1:145-146.

Kenney, M.C., M. Chwa, M. Escobar and D.J. Brown: (1991) Characterization of the major matrix degrading metalloproteinase of human corneal stroma; evidence for an enzyme/inhibitor complex. Exp. Eye Res. 52:5-16.

Brown, D.J., M. Chwa, A.J. Opbroek and M.C. Kenney: (1993) Keratoconus corneas: Increased gelatinolytic activity appears after modification of inhibitors. Curr. Eye Res. 12:571-581.

Opbroek, A., M.C. Kenney and D.J. Brown: (1993) Characterization of a human corneal metalloproteinase inhibitor (TIMP-1). Curr. Eye Res. 12:877-883.

Brown, D.J., M. Chwa and M.C. Kenney: (1994) Altered gelatinolytic activities in an apparent unilateral keratoconus patient. Cornea, 13:108-113.

Kenney, M.C., M. Chwa, A. Opbroek and D.J. Brown: (1994) Increased gelatinolytic activity in keratoconus keratocyte cultures: A correlation to an altered MMP-2/TIMP ratio. Cornea, 13:114-124.

Perng G.-C., J.C. Zwaagstra, D.J. Brown, A.B. Nesburn, and S.L. Wechsler: (1994) Similarities in regulation of the HSV-1 LAT promoter in corneal and neuronal cells. Invest. Ophthalmol. Vis. Sci. 35:2981-2989.

Brown, D.J., H. Hamdi and M.C. Kenney: (1995) Characterization of an endogenous metalloproteinase in human vitreous. Curr. Eye Res. 13:639-647.

Hamdi, H., Berjis, F., Brown, D.J. and Kenney, M.C: (1995) Proteinase activity in normal human tears: Male-female dimorphism. Curr. Eye Res. 14:1081-1086.

Brown, DJ, Bishop, P, Hamdi, H and Kenney, MC: (1996) Cleavage of structural components of mammalian vitreous by endogenous matrix metalloproteinase-2. Curr. Eye Res. 15:439-446.

Myint, E, Brown, DJ, Ljubimov, AV, Kyaw, M and Kenney, MC: (1996) Cleavage of human corneal type VI collagen ?3 chain by matrix metalloproteinase-2. Cornea, 15:490-496.

M.C. Kenney, M. Chwa, H. Kwan and Brown, DJ: (1996) Altered type VI collagen synthesis by keratoconus keratocytes in vitro. Biochem. Biophys. Res. Commun. 224: 760-764.

Kenney MC, Chwa M, Alba A, Saghizadeh M, Huang Z-S and Brown DJ: (1998) Localization of TIMP-1, TIMP-2, TIMP-3, gelatinase A and gelatinase B in pathological human corneas. Curr. Eye Res. 17:238-246.

Chiplunkar S, Chamblis K, Chwa M, Huang GH, Kenney MC and Brown DJ: (1999) Selective expression of a transmembrane phosphotyrosine phosphatase (LAR) in keratoconus corneas. Exp. Eye Res. 68; 283-293.

Spirin KS, Ljubimov AV, Castellon R, Wiedoeft O, Marano M, Sheppard D, Kenney MC and Brown DJ: (1999) Analysis of gene expression in human bullous keratopathy corneas containing limiting amounts of RNA. Invest. Ophthalmol. Vis. Sci. 40:3108-3115.

Kenney MC, Brown DJ and Rajeev B: (2000) Everett Kinsey Lecture. The elusive causes of keratoconus: A working hypothesis. The CLAO Journal. 26:10-13.

Saghizadeh M, Brown DJ, Castellon R, Chwa M, Huang GH, Ljubimova JY, Rosenberg S, Spirin KS, Stolitenko RB, Adachi W, Kinoshita S, Murphy G, Windsor LJ, Kenney MC, Ljubimov AV: (2001) Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations. Am. J. Pathol. 158:723-734.

Kenney MC, Chwa M, Huang G, Ljubimov AV, and Brown DJ. (2001) Identification of cell types in human diseased corneas. Cornea. 20:309-316.

Saghizadeh M, Chwa M, Aoki A, Lin B, Pirouzmanesh A, Brown DJ, Ljubimov AV, and Kenney MC. (2001) Altered expression of growth factors and cytokines in keratoconus, bullous keratopathy and diabetic human corneas. Experimental Eye Research. 73:179-189.

Buddi R, Lin B, Atilano S, Zorapapel N, Kenney MC, and Brown DJ. (2002) Evidence of oxidative stress in human diseased corneas. Journal Histochemistry & Cytochemistry. 50:341-351.

Hamdi H, Reznik R, Castellon R, Atilano S, Ong J, Udar N, Tavis J, Aoki A, Nesburn A, Boyer D, Small K, Brown DJ, and Kenney MC. (2002) Alu DNA polymorphism in ACE gene is protective for age-related macular degeneration. Biochem Biophys Res Commun. 295:668-72.

Saghizadeh M, Brown DJ, Tajbakhsh J, Chen Z, Kenney MC, Farber SB and Nelson SF. (2003) Evaluation of techniques using amplified and non-amplified nucleic acid probes for gene expression profiling with cDNA microarrays. Biomolecular Engineering. 20:97-106.

Ong JM, Aoki AM, Zorapapel NC, Brown DJ, Nesburn AB, Rich KR and Kenney MC. (2003). Impaired electroretinogram (ERG) response in apolipoprotein E-deficient mice. Curr Eye Res. 27:15-24.

MC Kenney and DJ Brown. (2003). The Cascade Hypothesis of Keratoconus. Contact Lens & Anterior Eye 26:139-146.

Kenney MC, Zorapapel N, Atilano S, Chwa M, Ljubimov A, and Brown D.J. (2003). Insulin-like growth factor-I (IGF-I) and transforming growth factor-beta (TGF-beta) modulate tenascin-C and fibrillin-1 in bullous keratopathy stromal cells in vitro. Exp Eye Res. 77:537-46.

Pappa A, Estey T, Manzer R, Brown D, Vasiliou V. (2003). Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea. Biochem J. 376:615-623.

Udar N, Kenney MC, Chalukya M, Anderson T, Morales L, Brown D, Nesburn A, Small K. (2004). Keratoconus--no association with the transforming growth factor beta-induced gene in a cohort of American patients. Cornea. 23:13-17.

Brown D, Lin B, Chwa M, Atilano S, Kim D, Kenney M. (2004). Elements of the nitric oxide pathway can degrade TIMP-1 and increase gelatinase activity. Molecular Vision. 10:281-288.

Brown D, Lin B, Holguin B. (2004). Neuregulin 1, a member of the epidermal growth factor family, is expressed as multiple splice variants in the adult human cornea. Invest. Ophthalmol. Vis. Sci. 45:3021-3029.

Osorio Y, Hofman F, Brown D, Ghiasi H. (2004) Involvement of CD8+ T Cells in Exacerbation of Corneal Scarring in Mice. Curr. Eye Res. 29:145-151.

Kenney MC, Chwa M, Atilano SR, Tran A, Carballo M, Saghizadeh M, Vasiliou V, Adachi W, Brown DJ. (2005). Increased Levels of Catalase and Cathepsin V/L2 but Decreased TIMP-1 in Keratoconus Corneas: Evidence that Oxidative Stress Plays a Role in This Disorder. Invest Ophthalmol Vis Sci. 46:823-32.

Aglaia P, Brown DJ, Koutalos Y, DeGregori J, White C, and Vasiliou V. (2005) Human Aldehyde Dehydrogenase 3A1 (ALDH3A1) Inhibits Proliferation and Promotes Survival of Human Corneal Epithelial Cells. J. Biol. Chem. 29;280:27998-8006.

Luthra S, Narayanan R, Marques LE, Chwa M, Kim DW, Dong J, Seigel GM, Neekhra A, Gramajo AL, Brown DJ, Kenney MC, Kuppermann BD (2006) Evaluation of in vitro effects of Bevacizumab (Avastin) on ocular retinal pigment epithelial, neurosensory retinal, and microvascular endothelial cells. Retina. 26(5):512-518.

Udar N, Atilano SR, Brown DJ, Holguin B, Small K, Nesburn AB, Kenney MC. (2006). SOD1: A Candidate Gene for Keratoconus. Invest Ophthalmol Vis Sci. 47(8):3345-51.

Morishige N, Wahlert AJ, Kenney MC, Brown DJ, Kawamoto K, Chikama T, Nishida T, Jester JV. (2007) Second-harmonic imaging microscopy of normal human and keratoconus cornea. Invest Ophthalmol Vis Sci.. 48(3):1087-94.

Brown DJ, Morishige N, Neekhra A, Minckler DS, Jester JV. (2007) Application of second harmonic imaging microscopy to assess structural changes in optic nerve head structure ex vivo. J Biomed Opt. 12(2):024029.

Nesburn AB, Bettahi I, Dasgupta G, Chentoufi AA, Zhang X, You S, Morishige N, Wahlert AJ, Brown DJ, Jester JV, Wechsler SL, Benmohamed L. (2007) Functional Foxp3+ CD4+CD25(Bright+) "Natural" Regulatory T Cells are Abundant in Rabbit Conjunctiva and Suppress Virus-Specific CD4+ and CD8+ Effector T Cells During Ocular Herpes Infection. J Virol. 14:7647-61.

Carpenter D, Hsiang C, Brown DJ, Jin L, Osorio N, Benmohamed L, Jones C, Wechsler SL. (2007). Stable cell lines expressing high levels of the herpes simplex virus type 1 LAT are refractory to caspase 3 activation and DNA laddering following cold shock induced apoptosis. Virology. 369:12-8.

Mott K, Osorio Y, Brown DJ, Morishige N, Wahlert A, Jester JV, and Ghiasi H. (2007). The Corneas of Naive Mice Contain Both CD4+ and CD8+ T Cells. Molecular Vision, 13:1802-10.

Application of non-linear optics and two-photon excited fluorescence to assess structural components in the Optic Nerve Head.”
1/01/08 - 12/31/10 Co-Investigator – “Corneal HSV-1: Immunopathologic Mechanisms of HSK” – NIH EY018171.
4/01/06 - 3/31/11 Co-Investigator – “Role of TGFb in Corneal Stromal Wound Healing” – NIH EY07348.
1/01/07 – 12/31/07 Co-Investigator – “Dynamic 3D Changes in Lamina Cribrosa using Second Harmonic Imaging Microscopy.” Glaucoma Foundation.
9/01/07 – 8/31/10 Principal Investigator – “Pressure Induced Dynamic 3D Changes in Lamina Cribrosa.” NIH EY017959.
09/01/09 – 08/31/10 Principal Investigator: “Structure and biomechanics of the optic nerve head” Discovery Eye Foundation.
4/01/10-3/31/14 Principal Investigator – “Structure and Biomechanics of the Human Optic Nerve Head.” NIH EY019719
1/01/07 – 12/31/07 Principal Investigator – “Pressure Induced Dynamic 3D Changes in Lamina Cribrosa using Second Harmonic Imaging Microscopy.” Glaucoma Research Foundation
7/01/04 – 6/30/04 Principal Investigator – “Role of Neuregulin in Corneal Wound Healing” Council on Research
Title: Age-Related Meibomian Gland Dysfunction. 5R01EY021510 Principal Investigator: James V. Jester, Ph.D. Agency: NIH/NEI Period: 09/30/11 – 08/31/15
Title: Mesenchymal Stem Cell Therapy for Corneal Cystinosis. 1R01EY022365 Principal Investigator: Jennifer Simpson, M.D. Agency: NIH/NEI Period: 04/01/12 – 03/31/16
Title: Corneal HSV-1: LAT’s Anti-apoptosis Activity and Latency. 5R01EY013191 Principal Investigator: Steven Wechsler, Ph.D. Agency: NIH/NEI Period: 07/01/10 – 05/31/14
Professional Societies
Association for Research in Vision and Ophthalmology
International Society for Eye Research.
Research to Prevent Blindness
Other Experience
Research Scientist II
Ophthalmology Research Laboratories, Cedars-Sinai Medical Center 1993—2002

Research Scientist I
Cedars-Sinai Medical Center 1988—1993

Research Virologist
Dept.Microbiology and Immunology, UCLA 1986—1988

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