Hiroaki Tomita


Head, Laboratory of Human Genetics, Psychiatry & Human Behavior
School of Medicine


M.D., Okayama University, 1989, Psychiatry
Ph.D., Okayama University, 1995, Medical Science

Phone: (949) 824-2670
Fax: (949) 824-7012
Email: htomita@uci.edu

University of California, Irvine
3113 Gillespie
Mail Code: 1675
Irvine, CA 92697
Research Interests
Genetic factors susceptible to psychiatric disorders
Academic Distinctions
Trousdale Research Scholarship, University of California, Irvine, 2000
Della Martin Research Scholarship, University of California, Irvine, 2001
Future Leaders in Psychiatry, Janssen Pharmaceutica, 2002
Research Abstract
Major interest of Dr. Tomita is to elucidate genetic factors effect on neuropsychiatric disorders and other genetic traits, using both genetic approach and gene expression profiling. Dr. Tomita has been involved in projects to elucidate comprehensive gene expression pattern in post-mortem brains of patients with psychiatric disorders by DNA microarray techniques, and genetic factors related to psychiatric disorders. Dr. Tomita has also been analyzing genetic factor and gene expression pattern of candidate genes susceptible to neuropsychiatric disorders, including SKCa3 (small conductance calcium activated potassium channel 3) gene as a candidate susceptible gene for schizophrenia. Dr. Tomita has also been involved in positional cloning of genetic disorders, including paroxysmal kinesigenic choreoathetosis (epileptic disorder), and genetic traits including wet/dry earwax trait.
Publications
1. Tomita H, Vawter MP, Walsh DM, Evans SJ, Choudary PV, Li J, Overman KM, Atz ME, Myers RM, Jones EG, Watson SJ, Akil H, Bunney WE Jr. Effect of agonal and postmortem factors on gene expression profile: quality control in microarray analyses of postmortem human brain. Biol Psychiatry. 2004; 55(4):346-52.

2. Tomita H, Shakkottai1 VG, Gutman GA, Sun G, Bunney WE, Cahalan MD, Chandy KG, Gargus JJ. Novel truncated isoform of SK3 potassium channel is a potent dominant-negative regulator of SK currents: implications in schizophrenia Molecular Psychiatry (2003) 8(5), 524-35.

3. Tomita H, Yamada K, Ghadami M, Ogura T, Yanai Y, Nakatomi K, Sadamatsu M, Masui A, Kato N, Niikawa N. The wet/dry earwax locus maps to the pericentromeric region of chromosome 16. Lancet 2002; 359: 2000-2002.

4. Miller MJ, Rauer H, Tomita H, Rauer H, Gargus JJ, Gutman GA, Cahalan MD, Chandy KG. Nuclear localization and dominant-negative suppression by a mutant SKCa3 N-terminal channel fragment identified in a patient with schizophrenia. J Biol Chem. 2001; 276(30):27753-27756.

5. Kinoshita A, Saito T, Tomita H, Makita Y, Yoshida K, Ghadami M, Yamada K, Kondo S, Ikegawa S, Nishimura G, Fukushima Y, Nakagomi T, Saito H, Sugimoto T, Kamegaya M, Hisa K, Murray JC, Taniguchi N, Niikawa N, Yoshiura KI. Domain-specific mutations in TGFB1 result in Camurati-Engelmann disease. Nature Genet, 2000; 26(1): 19-20.

6. Ghadami M, Makita Y, Yoshida K, Nishimura G, Fukushima Y, Wakui K, Ikegawa S, Yamada K, Kondo S, Niikawa N, Tomita H. Genetic mapping of the Camurati-Engelmann disease locus to chromosome 19q13.1-q13.3. Am J Hum Genet. 2000; 66(1):143-147.

7. Tomita H, Nagamitsu S, Wakui K, Fukushima Y, Yamada K, Sadamatsu M, Masui A, Konishi T, Matsuishi T, Aihara M, Shimizu K, Hashimoto K, Mineta M, Matsushima M, Tsujita T, Saito M, Tanaka H, Tsuji S, Takagi T, Nakamura Y, Nanko S, Kato N, Nakane Y, Niikawa N. Paroxysmal kinesigenic choreoathetosis locus maps to chromosome 16p11.2-q12.1. Am J Hum Genet. 1999; 65(6):1688-1697.

8. Evans SJ, Choudary PV, Neal CR, Li JZ, Vawter MP, Tomita H, Lopez JF, Thompson RC, Meng F, Stead JD, Walsh DM, Myers RM, Bunney WE, Watson SJ, Jones EG, Akil H. Dysregulation of the fibroblast growth factor system in major depression. Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0406788101

9. Wittekindt OH, Visan V, Tomita H, Imtiaz F, Gargus JJ, Lehmann-Horn F, Grissmer S, Morris-Rosendahl DJ. An Apamin- and Scyllatoxin-Insensitive Isoform of the Human SK3 Channel. Mol Pharmacol. 2004; 65(3):788-801.

10. Li JZ, Vawter MP, Walsh DM, Tomita H, Evans SJ, Choudary PV, Lopez JF, Avelar A, Shokoohi V, Chung T, Mesarwi O, Jones EG, Watson SJ, Akil H, Bunney WE Jr, Myers RM. Systematic changes in gene expression in postmortem human brains associated with tissue pH and terminal medical conditions. Hum Mol Genet. 2004 Mar 15;13(6):609-16. Epub 2004 Jan 20

11. Kolski-Andreaco A, Tomita H, Shakkottai VG, Gutman GA, Cahalan MD, Gargus JJ, Chandy KG. SK3-1C, a Dominant-negative Suppressor of SKCa and IKCa Channels. J Biol Chem. 2004 Feb 20;279(8):6893-904. Epub 2003 Nov 24.

12. Vawter M, Evans SJ, Choudary PV, Tomita H, Woodruff JM, Molnar M, Li J, Lopez JF, Myers R, Cox D, Watson SJ, Akil H, Jones EG, William E. Bunney WE. Gender specific gene expression in postmortem human brain: localization to sex chromosomes. Neuropsychopharmacology 2004; 29(2): 373-84.

13. Kondoh S, Sugawara H, Harada N, Matsumoto N, Ohashi H, Sato M, Kantaputra PN, Ogino T, Tomita H, Ohta T, Kishino T, Fukushima Y, Niikawa N, Yoshiura K. A novel gene is disrupted at a 14q13 breakpoint of t (2; 14) in a patient with mirror-image polydactyly of hands and feet. J Hum Genet 2002; 47(3): 136-139.

14. Kurotaki N, Imaizumi K, Harada N, Masuno M, Kondoh T, Nagai T, Ohashi H, Naritomi K, Tsukahara M, Makita Y, Sugimoto T, Sonoda T, Hasegawa T, Chinen Y, Tomita H, Kinoshita A, Mizuguchi T, Yoshiura Ki K, Ohta T, Kishino T, Fukushima Y, Niikawa N, Matsumoto N. Haploinsufficiency of NSD1 causes Sotos syndrome. Nature Genet 2002; 30(4): 365-366.

15. Shakkottai VG, Regaya I, Wulff H, Fajloun Z, Tomita H, Fathallah M, Cahalan MD, Gargus JJ, Sabatier JM, Chandy KG. Design and characterization of a highly selective peptide inhibitor of the small conductance calcium-activated K+ channel, SkCa2. J Biol Chem. 2001;276(46):43145-43151.

16. Ghadami M, Majidzadeh-A K, Haerian BS, Damavandi E, Yamada K, Pasallar P, Najafi MT, Nishimura G, Tomita H, Yoshiura KI, Niikawa N. Confirmation of genetic homogeneity of syndactyly type 1 in an Iranian family. Am J Med Genet. 2001;104(2):147-151.

17. Sun G, Tomita H, Shakkottai VG, Gargus JJ. Genomic organization and promoter analysis of human KCNN3 gene. J Hum Genet. 2001; 46(8):463-470.

18. Ghadami M, Tomita H, Najafi MT, Damavandi E, Farahvash MS, Yamada K, Majidzadeh AK, Niikawa N. Bardet-Biedl syndrome type 3 in an Iranian family: clinical study and confirmation of disease localization. Am. J. Med. Genet. 2000; 94: 433-437.

19. Yamada K, Tomita H, Yoshiura K, Kondo S, Wakui K, Fukushima Y, Ikegawa S, Nakamura Y, Amemiya T, Niikawa N. An autosomal dominant posterior polar cataract locus maps to human chromosome 20p12-q12. Eur J Hum Genet. 2000; 8(7):535-539.

20. Yamada K, Tomita H, Kanazawa S, Mera A, Amemiya T, Niikawa N. Genetically distinct autosomal dominant posterior polar cataract in a four-generation Japanese family. Am J Ophthalmol. 2000; 129(2):159-165.

21. Miyoshi O, Hayashi S, Fujimoto M, Tomita H, Sohda M, Niikawa N. Maternal uniparental disomy for chromosome 14 in a boy with intrauterine growth retardation. J Hum Genet 1998; 43(2):138-142.

22. Fujimoto M, Kantaputra PN, Ikegawa S, Fukushima Y, Sonta S, Matsuo M, Ishida T, Matsumoto T, Kondo S, Tomita H, Deng HX, D'urso M, Rinaldi MM, Ventruto V, Takagi T, Nakamura Y, Niikawa N. The gene for mesomelic dysplasia Kantaputra type is mapped to chromosome 2q24-q32. J Hum Genet 1998; 43(1):32-36.

23. Fujiwara Y, Yamaguchi K, Tanaka Y, Tomita H, Shiro Y, Kashihara K, Sato K, Kuroda S.
Polymorphism of dopamine receptors and transporter genes in neuropsychiatric diseases. Eur Neurol 1997; 38 Suppl 1:6-10.

24. Fujimoto M, Matsumoto N, Tsujita T, Tomita H, Kondo S, Miyake N, Nakano M, Niikawa N. Characterization of the promoter region, first ten exons and nine intron-exon boundaries of the DNA-dependent protein kinase catalytic subunit gene, DNA-PKcs (XRCC7). DNA Res 1997; 28;4(2):151-154.

25. Matsumoto N, Soeda E, Ohashi H, Fujimoto M, Kato R, Tsujita T, Tomita H, Kondo S, Fukushima Y, Niikawa N. A 1.2-megabase BAC/PAC contig spanning the 14q13 breakpoint of t(2; 14) in a mirror-image polydactyly patient. Genomics 1997; 45(1):11-16.

26. Tomita H, Hikiji M, Fujiwara Y, Akiyama K, Otsuki S. Changes in dopamine D2 and GluR-1 glutamate receptor mRNAs in the rat brain after treatment with phencyclidine. Acta Med Okayama 1995; 49(2):61-68.

27. Hikiji M, Tomita H, Ono M, Fujiwara Y, Akiyama K. Increase of kainate receptor mRNA in the hippocampal CA3 of amygdala-kindled rats detected by in situ hybridization. Life Sci 1993; 53(10):857-864.

28. Fujiwara Y, Tomita H, Hikiji M, Kashihara K, Otsuki S, Ohnuki T, Hamagishi Y, Oki T, Sora I, Roeske WR. Characterization of a cloned rat serotonin 5-HT1A receptor expressed in the HeLa cell line. Life Sci 1993; 52(11):949-958.

29. Sora I, Fujiwara Y, Tomita H, Ishizu H, Akiyama K, Otsuki S, Yamamura HI. Lack of effect of haloperidol or methamphetamine treatment on the mRNA levels of two dopamine D2 receptor isoforms in rat brain. Jpn J Psychiatry Neurol 1992; 46(4):967-973.

30. Fujiwara Y, Sora I, Tomita H, Akiyama K, Otsuki S, Yamamura HI. Localization of the mRNAs for two dopamine D2 receptor isoforms in the rat brain. Jpn J Psychiatry Neurol 1991; 45(4):897-902.

31. Bunney WE, Bunney BG, Vawter MP, Tomita H, Li J, Evans SJ, Choudary PV, Myers RM, Jones EG, Watson SJ, Akil H. Microarray technology: a review of new strategies to discover candidate vulnerability genes in psychiatric disorders. Am J Psychiatry. 2003; 160(4):657-66. Review

32. Tomita H. Recent advance in molecular genetics of mental disorder. Encyclopedia of Clinical Psychiatry, special issue vol 11, 2000; 191-200, Nakayama shoten, Tokyo, Review.

33. Tomita H, Niikawa N. Genomic Imprinting. Psychiatric review vol28, 1998 Life Science, Tokyo, Review.

34. Tomita H, Niikawa N. Mental Retardation and Congenital Malformation Disorder. Psychiatric review vol27, 1997 Life Science, Tokyo, Review.

35. Tomita H, Niikawa N. Molecular genetics of Prader-Willi syndrome and Bardet-biedl syndrome. Internal medicine up-to-date progressive series vol 2, 1997, Nakayama shoten, Tokyo, Review.

36. Fujiwara Y, Tomita H. Dopamine receptor. Recent Advance in Neuroscience 37(3): 443-458 (1993) Review.

37. Fujiwara Y, Sora I, Tomita H. The molecular biology of dopamine receptors. Yakubutsu Seishin Kodo. 1991 11(3):187-96. Review.
Professional Societies
Member of the American Society of Human Genetics
Member of the Society for Neuroscience
Last updated
02/07/2006