Edwin S Monuki

picture of Edwin S Monuki

Professor, Pathology
School of Medicine
Joint Appointment, Developmental & Cell Biology
School of Biological Sciences
Warren L. Bostick Chair, Pathology
School of Medicine
Staff Neuropathologist, UCI Medical Center

M.D., University of California, San Diego
Ph.D., University of California, San Diego

Phone: (949) 824-9604
Fax: (949) 824-2160
Email: emonuki@uci.edu

University of California, Irvine
Department of Pathology
D440 Med Sci I
Mail Code: 4800
Irvine, CA 92697
Research Interests
Cerebral cortex, stem cells, development, neurobiology, neuropathology, mouse transgenics, BMP, patterning, brain malformations
Research Abstract
The goal of our laboratory is to understand how the cerebral cortex and its neural stem cells (NSCs) develop, then apply this information to human disorders and culture strategies for potential cell-based therapies. Current studies focus on the roles of morphogens and transcription factors in early NSC fate control and boundary formation involving the cortex and specialized tissues at the midline. A midline tissue of particular interest is the choroid plexus, the source of cerebrospinal fluid (CSF) and a tissue with untapped therapeutic potential. Approaches used in the lab include mouse genetics, mouse models of human disease, in utero electroporation, explants, and mouse and human NSC and embryonic stem (ES) cell cultures. In collaboration with other UCI labs, we are also using mathematical models to understand early forebrain development, bioengineering tools to study NSC responses to microfluidic morphogen gradients and mechanical tension, and novel strategies to sort and study NSCs based on their electrical signatures.
Available Technologies
Most Recent Publications
McGowan L, Kuo E, Martin A, Monuki ES, Striedter G. Species differences in early patterning of the avian brain. Evolution, in press.
Chizhikov VV, Lindgren AG, Mishima Y, Roberts RW, Aldinger KA, Miesegaes GR, Currle DS, Monuki ES, Millen KJ. Lmx1a regulates fates and location of cells originating from the cerebellar
rhombic lip and telencephalic cortical hem. PNAS 2010;107:10725-30.
Yun S, Saijoh Y, Hirokawa KE, Kopinke D, Murtaugh LC, Monuki ES, Levine EM. Lhx2 links the intrinsic and extrinsic factors controlling optic cup formation. Development 2009;136:3895-906.
Mutch C, Funatsu N, Monuki E, Chenn A. Beta-catenin signaling levels in progenitors regulate the laminar cell fates of projection neurons. J Neurosci 2009;29:13710-9.
Wang L, Lu J, Marchenko SA, Monuki ES, Flanagan LA, Lee AP. Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow-through separation of beads and cells.
Electrophoresis 2009;30:782-91.
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flugel A, Pennington MW, Parker I, Chandy KG, Cahalan MD. Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block. Immunity 2008;29:602-14.
Flanagan LA, Lu J, Wang L, Marchenko SA, Jeon NL, Lee AP, Monuki ES. Unique dielectric properties distinguish stem cells and their differentiated progeny. Stem Cells 2008;26:656-65.
(Additional coverage on NPR and in Wired Magazine.)
Hu JS, Doan L, Currle DS, Paff M, Rheem J, Schreyer R, Robert B, Monuki ES. Border formation in a Bmp gradient reduced to single dissociated cells. Proc Natl Acad Sci 2008;105:3398-403.
Mangale VS, Hirokawa KE, Satyaki PRV, Gokulchandran N, Chickbire S, Subramanian L, Shetty AS, Martynoga B, Paul J, Mai MV, Li Y, Flanagan LA, Tole S, Monuki ES. Lhx2 selector activity specifies cortical identity and suppresses hippocampal organizer fate. Science 2008;319:304-9.
Flanagan LA, Lu J, Wang L, Marchenko SA, Jeon NL, Lee AP, Monuki ES. Unique dielectric properties distinguish stem cells and their differentiated progeny. Stem Cells 2008;26:656-65.
Chung BG, Park JW, Hu JS, Huang C, Monuki ES, Jeon NL. A hybrid microfluidic device for direct interfacing with wet cell culture methods. BMC Biotechnol 2007;7:60.
Wang L, Flanagan LA, Jeon NL, Monuki ES, Lee AP. Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry. Lab Chip 2007;7:1114-20.
Cheng X, Hsu CM, Currle DS, Hu JS, Barkovich AJ, Monuki ES. Central roles of the roof plate in telencephalic development and holoprosencephaly. J Neurosci 2006;26:7640-7649.
Flanagan L, Rebaza L, Derzic S, Schwartz P, Monuki ES. Regulation of human neural precursor cells by laminin and integrins. J Neurosci Res 2006;83:845-856.
Chung BG, Flanagan LA, Rhee SW, Schwartz PH, Lee AP, Monuki ES, Jeon NL. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip 2005;5:401-406.
Currle DS, Hsu CM, Cheng X, Monuki ES. Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelia formation. Development 2005;132:3549-3559.
NIH R01 NS064587, "The Morphogen and Selector Gene Network in the Dorsal Telencephalic Midline"
California Institute for Regenerative Medicine (CIRM) RN2-00915-1, "Mechanisms in Choroid Plexus Development"
Professional Societies
Society for Neuroscience
American Association of Neuropathologists
Graduate Programs
Interdepartmental Neuroscience Program

Mathematical and Computational Biology

Cellular and Molecular Biosciences

Research Centers
Developmental Biology Center
Reeve-Irvine Spinal Cord Injury Center
Epilepsy Center (EpiCenter)
Center for Complex Biological Systems
UCI Memory Impairments and Neurological Disorders (MIND)
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