Kivie Moldave

Professor Emeritus, Molecular Biology and Biochemistry
School of Biological Sciences

B.S., University of California, Berkeley


Ph. D., University of Southern California

Phone: 949-824-3446
Fax: 949-824-8551
Email: kmoldave@uci.edu

University of California, Irvine
473 Steinhouse Hall
Mail Code: 3900
Irvine, CA 92697


Research
Interests
Enzymologial, molecular and genetic aspects of protein biosynthesis in eukaryotic systems.
   
Academic
Distinctions
USPHS Career Award (1963); W.S. McEllroy Professor of Biochemistry, Univ. of Pittsburgh (1966-1970); Fellow, American Association for the Advancement of Science (1986); Organization of American States Professorship, University of Mexico (1972); Research Scholar of the American Cancer Society (1973); Sigma Xi, Honorary Research Society (1951); Phi Lambda Epsilon, Honorary Chemical Society (1952); Alpha Omega Alpha, Honorary Medical Society (1984)

Appointments:
Postdoctoral Fellow, University of Wisconsin, Madison, WI (1952-1953); Postdoctoral Fellow, Faculte des Sciences, Universite de Paris, France (1953-1954)Assistant Professor-Professor, Department of Biochemistry, Tufts University School of Medicine, Boston, MA (1954-1966); Professor and Chair, Department of Biochemisty, University of Pittsburgh School of Medicine, Pittsburgh PA (1966-1970); Professor and Chair, Department of Biological Chemistry, University of California, Irvine School of Medicine (1970-1980); Professor, Department of Biological Chemistry, University of California, Irvine School of Medicine (1980-1984); Academic Vice Chancellor and Professor of Biology, University of California, Santa Cruz (1984-1991); Professor Emeritus, Department of Molecular Biology and Biochemistry, University of California, Irvine (1992- ). Visiting Professorships: University of Copenhagen, Denmark (1963); Hebrew University of Jerusalem, Israel (1971, 1973); National Autonomous University of Mexico, Mexico (1972); University of British Columbia, Vancouver, Canada (1972); Imperial Cancer Research Laboratories, London, England (1981); Cornell University Medical College, NY (1982); Institut Jacques Monod, Paris, France (1988); National Institute for Medical Research, London, England (1989, 1990).
   
Appointments Postdoctoral Fellow, University of Wisconsin, Madison, WI (1952-1953); Postdoctoral Fellow, Faculte des Sciences, Universite de Paris, France (1953-1954)Assistant Professor-Professor, Department of Biochemistry, Tufts University School of Medicine, Boston, MA (1954-1966); Professor and Chair, Department of Biochemisty, University of Pittsburgh School of Medicine, Pittsburgh PA (1966-1970); Professor and Chair, Department of Biological Chemistry, University of California, Irvine School of Medicine (1970-1980); Professor, Department of Biological Chemistry, University of California, Irvine School of Medicine (1980-1984); Academic Vice Chancellor and Professor of Biology, University of California, Santa Cruz (1984-1991); Professor Emeritus, Department of Molecular Biology and Biochemistry, University of California, Irvine (1992- ). Visiting Professorships: University of Copenhagen, Denmark (1963); Hebrew University of Jerusalem, Israel (1971, 1973); National Autonomous University of Mexico, Mexico (1972); University of British Columbia, Vancouver, Canada (1972); Imperial Cancer Research Laboratories, London, England (1981); Cornell University Medical College, NY (1982); Institut Jacques Monod, Paris, France (1988); National Institute for Medical Research, London, England (1989, 1990).
   
Research
Abstract
My intrests have been in the mechanism, regulation and genetics of protein biosynthesis in eukaryotic cells. My laboratory used monoclonal antibodies prepared against translational protein factors that are essential for protein synthesis and conditional-lethal mutants of yeast and cultured mammalian cells, in order to elucidate this process.


Cloned antibody-producing hybridoma cell lines, which produce immunoglobulins against nonribosomal translational components, were prepared. Antibodies from these cell lines were purified and tested for their effects on translation in homologous systems from yeast and from cultured Chinese hamster ovary cells. Those that inhibited protein synthesis were then used to identify the translational components using "Western" blotting and immunoaffinity chromatography. After isolation of the component, the structure, site of action, and function were investigated using a strategy and methodology, developed in my laboratory, for the analysis of the intermediary reations involved in the initiation, elongation and termination of protein synthesis.


Using a similar strategy and methodology, the genetic lesions in several temperature-sensitive mutant cells were investigated. A number of mutants were identified and examined, with altered components required for initiation, elongation, aminoacylation of tRNA and so forth.


Detailed structure-function investigations of the translational components - and their genes - that are genetically altered in the mutant cell collection and of those that interact with specific monoclonal antibodies in that collection were initiated.
   
Publications Editor, Progress in Nucleic Acid Research and Molecular Biology; Academic Press, Inc., Volumes 30-80 (1983-2005)
   
  Screening a Yeast Promoter Library Leads to the Isolation of the RP29/L32 and SN17B/RPL37A Divergent Promoters and the Discovery of a Gene Encoding Ribosomal Protein L37; Gene, V105, pgs. 137-138 (1991)
   
  Discovery, Resolution, Purification and Function of the Elongation Factors; Methods in Enzymology, V182, pgs. 809-818 (1990)
   
  Properties of Promoters Cloned Randomly from the Saccharomyces cerevisiae Genome; Mol. Cell. Biol. V8, pgs.4217-4224 (1988)
   
  The Analysis of Temperature-sensitive Mutants of Saccharomyces cerevisiae Altered in Components Required for Protein Synthesis: New Approaches; Springer Verlag pgs. 271-281 (1988)
   
  Isolation of the Yeast Gene Encoding Elongation Factor 3 for Protein Synthesis; J. Biol. Chem. V262, pgs. 7802-7808 (1987)
   
  Cloning of Open Reading Frames and Promoters from the Saccharomyces cerevisiae Genome: Construction of Genomic Libraries of Random Small Fragments; Gene, V46, pgs. 181-186 (1986)
   
  Eukaryotic Protein Synthesis; Ann. Rev. Biochem. V54, pgs. 1109-1149 (1985)
   
  Identification of an Altered Elongation Factor in Temperature-sensitive Mutant ts7'-14 of Saccharomyces cerevisiae; J. Biol. Chem. V259, pgs. 14347-14349 (1984)
   
  A Competitive Solid-phase Radioimmunoassay for Translational Factors Employing Monoclonal Antibodies; J. Immunol. V73, pgs. 337-345 (1984)
   
Professional
Societies
American Society for Biochemistry and Molecular Biology
American Chemical Society
Sigma Xi, Honorary Research Society
Phi Lambda Epsilon, Honorary Chemical Society
Amer. Ass'n for the Advancement of Science; Fellow 1986
Alpha Omega Alpha, Honorary Medical Society
   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=4598
   
Last updated 02/14/2005