Haoping Liu

Professor, Biological Chemistry
School of Medicine

PH.D., Cornell University, 1991

Phone: (949) 824-1137
Fax: (949) 824-2688
Email: h4liu@uci.edu

University of California, Irvine
D250, D288 MED SCI I
Irvine, CA 92697

Signaling pathways; dimorphism, phenotypic switching and pathogenesis of Candida albicans; mechanisms of drug resistance; Candida-host interaction
URL www.ucihs.uci.edu/biochem/faculty/liu.html
Candida albicans is one of the most frequently isolated fungal pathogens in humans. It causes a wide variety of infections ranging from mucosal infections in generally healthy people to life-threatening systemic infections in immunocompromised individuals. C. albicans is polymorphic and undergoes reversible morphogenetic transitions between budding, pseudohyphal and hyphal growth forms. Its ability to switch growth forms is important for the virulence of C. albicans. A major emphasis of our research has been to understand how C. albicans senses host signals and regulates its growth forms and ability to survive and infect at different host sites.
Another area of our research is antifungal drug discovery and mechanisms of drug resistance.
Available Technologies
Publications 1. Liu, H., and Bretscher, A. (1989). Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and Physarum. Proc Natl Acad Sci U S A 86, 90-93.
2. Liu, H., and Bretscher, A. (1989). Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell 57, 233-242.
3. Liu, H., and Bretscher, A. (1992). Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport. J Cell Biol 118, 285-299.
4. Liu, H., Krizek, J., and Bretscher, A. (1992). Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast. Genetics 132, 665-673.
5. Liu, H., Styles, C.A., and Fink, G.R. (1993). Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262, 1741-1744.
6. Liu, H., Kohler, J., and Fink, G.R. (1994). Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog. Science 266, 1723-1726.
7. Liu, H., Styles, C.A., and Fink, G.R. (1996). Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth. Genetics 144, 967-978.
8. Loeb, J.D., Kerentseva, T.A., Pan, T., Sepulveda-Becerra, M., and Liu, H. (1999). Saccharomyces cerevisiae G1 cyclins are differentially involved in invasive and pseudohyphal growth independent of the filamentation mitogen-activated protein kinase pathway. Genetics 153, 1535-1546.
9. Loeb, J.D., Sepulveda-Becerra, M., Hazan, I., and Liu, H. (1999). A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans. Mol Cell Biol 19, 4019-4027.
10. Chen, J., Zhou, S., Wang, Q., Chen, X., Pan, T., and Liu, H. (2000). Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans. Mol Cell Biol 20, 8696-8708.
11. Lane, S., Birse, C., Zhou, S., Matson, R., and Liu, H. (2001). DNA array studies demonstrate convergent regulation of virulence factors by Cph1, Cph2, and Efg1 in Candida albicans. J Biol Chem 276, 48988-48996.
12. Lane, S., Zhou, S., Pan, T., Dai, Q., and Liu, H. (2001). The basic helix-loop-helix transcription factor Cph2 regulates hyphal development in Candida albicans partly via TEC1. Mol Cell Biol 21, 6418-6428.
13. Liu, H. (2001). Transcriptional control of dimorphism in Candida albicans. Curr Opin Microbiol 4, 728-735.
14. Hazan, I., Sepulveda-Becerra, M., and Liu, H. (2002). Hyphal elongation is regulated independently of cell cycle in Candida albicans. Mol Biol Cell 13, 134-145.
15. Liu, H. (2002). Constructing yeast libraries. Methods Enzymol 350, 72-86.
16. Chen, J., Lane, S., and Liu, H. (2002). A conserved mitogen-activated protein kinase pathway is required for mating in Candida albicans. Mol Microbiol 46, 1335-1344.
17. Liu, H. (2002). Co-regulation of pathogenesis with dimorphism and phenotypic switching in Candida albicans, a commensal and a pathogen. Int J Med Microbiol 292, 299-311.
18. Hazan, I., and Liu, H. (2002). Hyphal tip-associated localization of Cdc42 is F-actin dependent in Candida albicans. Eukaryot Cell 1, 856-864.
19. Hazan, I., and Liu, H. (2004). Regulation of morphogenesis by conserved developmental pathways in pathogenic fungi. In Pathogenic Fungi, G. San-Blas and R.A. Calderone, eds. (Caister Academic Press).
20. Ni, J., Gao, Y., Liu, H., and Chen, J. (2004). Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production. FEBS Lett 561, 223-230.
21. Chou, S., Huang, L., and Liu, H. (2004). Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeast. Cell 119, 981-990.
22. Cao, F., Lane, S., Raniga, P.P., Lu, Y., Zhou, Z., Ramon, K., Chen, J., and Liu, H. (2006). The Flo8 transcription factor is essential for hyphal development and virulence in Candida albicans. Mol Biol Cell 17, 295-307.
23. Mao, X., Cao, F., Nie, X., Liu, H., and Chen, J. (2006). The Swi/Snf chromatin remodeling complex is essential for hyphal development in Candida albicans. FEBS Lett 580, 2615-2622.
24. Chou, S., Lane, S., and Liu, H. (2006). Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol 26, 4794-4805.
25. Huang, G., Wang, H., Chou, S., Nie, X., Chen, J., and Liu, H. (2006). Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans. Proc Natl Acad Sci U S A 103, 12813-12818.
26. Wang, A., Lane, S., Tian, Z., Sharon, A., Hazan, I., and Liu, H. (2007). Temporal and spatial control of HGC1 expression results in Hgc1 localization to the apical cells of hyphae in Candida albicans. Eukaryot Cell 6, 253-261.
27. Lu, Y., Su, C., Mao, X., Raniga, P.P., Liu, H., *and Chen, J.* (2008). Efg1-mediated recruitment of NuA4 to promoters is required for hypha-specific Swi/Snf binding and activation in Candida albicans. Mol Biol Cell 19(10):4260-72. (*corresponding author)
28. Chou, S., Zhou, S., Song, Y., Liu, H.* and Nie, Q.* (2008). Fus3-triggered Tec1 degradation modulates mating transcriptional output during the pheromone response. Nature Molecular Systems Biology 4:212. (*corresponding author)
29. Mochon AB, Liu H. (2008) The antimicrobial peptide histatin-5 causes a spatially restricted disruption on the Candida albicans surface, allowing rapid entry of the peptide into the cytoplasm. PloS Pathogens. 4(10):e1000190.
30. Wang A., Raniga P.P., Lane S., Lu Y., Liu H. (2009). Hyphal chain formation in Candida albicans: Cdc28-Hgc1 phosphorylation of Efg1 represses cell separation genes, Mol Cell Biol. 29(16).
31. Park H., Liu Y., Solis N., Spotkov J., Hamaker J., Blankenship J.R., Yeaman M.R., Mitchell A. P., Liu H., Filler S.G. (2009) Transcriptional Responses of Candida albicans to Epithelial and Endothelial Cells. Eukaryotic Cell 8(10).
32. Lei J., He G., Liu H., Nie Q. (2009) A delay model for noise-induced bi-directional switching. Nonlinearity 22(12):2845-2859.
33. Mochon AB., Ye J., Kayala M.A., Wingard J.R., Clancy C.J., Nguyen, M.H., Felgner P., Baldi P., Liu H. (2010) Profiling antibody response with Candida albicans cell surface protein microarray reveals constant host-pathogen interplay and infection-associated antigens during systemic candidiasis. PLoS Pathogens 6(3):e1000827.
34. Lu Y. Su C. Wang A. & Liu H. (2011) Hyphal development in Candida albicans requires two temporally linked changes in promoter chromatin for initiation and maintenance. PLoS Biology 9(7):e1001105. PMID: 21811397.
35. Stevenson J.S. & Liu H. (2011) Regulation of white and opaque cell type formation in Candida albicans by Rtt109 and Hst3. Mol. Microbiology 81(4):1078-91. PMID: 21749487
36. Lu Y., Su C. & Liu H. (2012) A GATA Transcription Factor Recruits Hda1 In Response to Reduced Tor1 Signaling To Establish a Hyphal Chromatin State in Candida albicans. PLoS Pathogens 8(4):e1002663. PMID: 22536157.
37. Su C., Lu Y., Liu H. (2013) Reduced TOR Signaling Sustains Hyphal Development in Candida albicans by lowering Hog1 basal activity. Mol Biol Cell 24(3):385-97. PMID: 23171549.
38. Stevenson JS & Liu H. (2013) Nucleosome assembly factors CAF-1 and HIR modulate epigenetic switching frequencies in an H3K56 acetylation-associated manner in Candida albicans. Eukaryot Cell 12(4):591-603. PMID: 23417560.
39. Lu Y., Su C., Solis N.V., Filler S.G. & Liu H. (2013) Synergistic regulation of hyphal elongation by hypoxia, CO2, and nutrient conditions controls pathogenicity of Candida albicans. Cell Host & Microbes 14(5):499-509. PMID: 24237696.
40. Lu Y., Su C., Unoje O., Liu H. (2014) Quorum sensing controls hyphal initiation in Candida albicans through Ubr1-mediated protein degradation. Proc Natl Acad Sci 111(5):1975-80.
41. Lu Y., Su C., Liu H. Candida hyphal Initiation and elongation. Trends Microbiol. 2014 Dec;22(12):707-714. PMID: 25262420
42. Li X.*, Alspaugh JA*, Liu H. *, Harris S. * Fungal Morphogenesis. Cold Spring Harb Perspect Med doi: 10.1101/cshperspect.a019679, Published by Cold Spring Harbor Laboratory Press. 2014. * These authors contributed equally to this work.
43. Guan Z. & Liu H. Overlapping functions between SWR1 deletion and H3K56 acetylation in Candida albicans. Eukaryot Cell, 2015, PMID: 25862154
44. Guan Z. & Liu H. The WOR1 5’ untranslated region regulates white-opaque switching in Candida albicans by reducing translational efficiency. Mol. Microbiology. 2015 PMID: 25831958
45. Yan M, Nie X, Wang H, Gao N, Liu H, Chen J. SUMOylation of Wor1 by a novel SUMO E3 ligase controls cell fate in Candida albicans. Mol Microbiol. 2015. PMID: 26112173
46. Premachandra ID, Scott KA, Shen C, Wang F, Lane S, Liu H, Van Vranken DL. Potent Synergy Between Spirocyclic Pyrrolidinoindolinoses and Fluconazole against Candida albicans. ChemMedChem. 2015 PMID: 26263912
47. Lane S., Di Lena, Tormanen K. Baldi P. Liu H. Function and regulation of Cph2 in Candida albicans. Eukaryot Cell. 2015
48. Su C., Lu Y., Liu H., N-acetylglucosamine sensing by a GCN5-related N-acetyltransferase induces transcription via chromatin histone acetylation in fungi. Nature Communications. 7:12916. 2016
49. Mood A, Premachandra ID, Hiew S, Wang FQ, Scott K, Oldenhuis N, Liu H, Van Vranken D. Potent Antifungal Synergy of Phthalazinone and Isoquinolones with Azoles Against Candida albicans. ACS Medicinal Chemistry Letters, 2017.
Grants R01 AI099190 (2012-2018)
R01 GM117111-01A1, 2017-2021
Graduate Programs Cellular and Molecular Biosciences

Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=4547
Last updated 10/18/2017