Laser tweezers, optical tweezers. lasers, molecular motors, kinesin, dynein, cargo transport,myosin, drosophila, biophysics, cross-disciplinary, motor coordination
Cells are highly structured, and this structure is created and maintained using the cytoskeleton in conjunction with molecular motors. My research combines genetic and biochemical manipulations with biophysical techniques ( laser-tweezers, single-particle tracking and analysis, and computer modeling) to study cargo transport in vivo. My primary goal is to understand the extent to which the activity of different molecular motors is coordinated, and the detailed physical changes in motor activity that result in alteration of net cargo transport.
61. A. Kunwar, Kunwar, S.K. Tripathy, J. Xu, M.K. Mattson, P. Anand, R. Sigua, M. Vershinin, R.J. McKenney, C. C. Yu, A. Mogilner+†, and S.P. Gross+†, “Mechanical stochastic tug-of-war models cannot explain bidirectional lipid-droplet transport”, PNAS, November 14, (2011).
60. J. Yi, K. Ori-McKenney, R. McKenney, M. Vershinin, S.P. Gross, and R. B. Vallee, “High Resolution Imaging Reveals Indirect Coordination of Opposite Motors and LIS1 Role in High-load Axonal Transport.”, J. Cell Biol., (2011)
59. M. Bosch, M. Marí, S.P. Gross, J.C. Fernández-Checa, and A. Pol, “Mitochondrial cholesterol: a connection between caveolin and metabolism”, Traffic (AOP) (2011).
58. R.P. Erickson, Z. Jia, S.P. Gross+† and C.C. Yu+†, “How Molecular Motors are Arranged on a Cargo is Important for Vesicular Transport”, PLOS Computational Biology (2011). +=Co-senior author †=Corresponding author
57. J. Xu and S.P. Gross, “Biophysics of dynein in vivo” (invited review), to appear in Dyneins: Structure, Biology and Disease, (Elsevier), Editor Stephen M. King (2012).
56. M. Bosch, M. Marí, A. Herms, A. Fernández, A. Fajardo, A. Kassan, A. Giralt, A. Colell, D. Balgoma, E. Barbero, E. González-Moreno, N. Matias, F. Tebar, J. Balsinde, M. Camps, C. Enrich, S.P. Gross, C. García-Ruiz, E. Pérez-Navarro, J.C. Fernández-Checa, and A. Pol, “Caveolin-1 deficiency causes cholesterol dependent mitochondrial dysfunction and apoptotic susceptibility” , Current Biology (AOP) (2011
55. G. Shubeita and S.P. Gross, , “Intracellular Transport: Relating Single-Molecule Properties to in Vivo Function” (invited review), to appear in Comprehensive Biophsics, (Elsevier), Editor Ed. Egelman (2012).
54. K.M. Ori-McKenney*, J. Xu*, S.P. Gross+†, and R.B. Vallee+†, “A cytoplasmic dynein tail mutation impairs motor processivity”, Nature Cell Biology (AOP), (2010)
53. R.J. McKenney*, M. Vershinin*, A. Kunwar, R.B. Vallee+†, and S.P. Gross+†, “LIS1 and NudE Induce a Persistent Dynein Force-Producing State”, Cell 141: 304–314, (2010)
52. K.H. Bremner, J. Scherer, J. Yi, M. Vershinin, S.P. Gross, and R.B. Vallee, “Adenovirus transport via direct interaction of cytoplasmic dynein with the viral capsid hexon subunit”. Cell Host Microbe. 6(6):523-35. (2009)
51. M. Ingelmo-Torres, E. González-Moreno, A. Kassan, M. Hanzal-Bayer, F. Tebar, A. Herms, T. Grewal, J.F. Hancock, C. Enrich, M. Bosch, S.P. Gross, R.G. Parton and A. Pol, “Hydrophobic and Basic Domains Target Proteins to Lipid Droplets”, Traffic 10(12):1785-801 (2009)
50. R. Mallik and SP Gross, “Intracellular transport: how do motors work together?”, Curr Biol.19(10), (2009)
49. F. Ziebert, M. Vershinin, SP Gross, IS Aranson, “Collective alignment of polar filaments by molecular motors.” Eur Phys J E Soft Matter, 28(4), (2009)
48. G. Shubeita, S. Tran, J. Xu, M. Vershinin, S. Cermelli, S. Cotton, M. Welte, and S.P. Gross, “Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets” Cell 135(6), (2008).
47. K. Larsen, J. Xu, S. Cermelli, and S.P. Gross, “BicaudalD actively regulates microtubule motor activity in lipid droplet transport.”, PLoS one 3(11), (2009).
46. A. Kunwar, M. Vershinin, J. Xu, and S.P. Gross, “Stepping, Strain Gating, and an Unexpected Force-Velocity Curve for Multiple-Motor-Based Transport”, Current Biology 18, 1–11, August 26, 2008 (2008).
45. M.A. Welte and S. P. Gross, “Molecular motors: a traffic cop within?”, HFSP Journal in press, (2008).
44. M. Vershinin, J. Xu, D. Razafsky, S.J. King, and S.P. Gross, “Tuning microtubule-based transport through filamentous MAPs: the problem of dynein.”
Traffic, 9(6):882-92 (2008)
43. B.C. Carter, M. Vershinin, S.P. Gross, “A Comparison of Step-Detection Methods: How Well Can You Do?”
Biophys. Jl, 94(1):306-19, (2008).
42. S. P. Gross, M. Vershinin and G.T. Shubeita, “Cargo Transport: Two Motors Are Sometimes Better Than One”, Curr. Bio. v.17, R478-486 (2007).
41. S. P. Gross, “Molecular Motors: A Tale of Two Filaments”, Curr. Bio. v.17, R277-280 (2007).
40. D.Y. Petrov, R. Mallik, G.T. Shubeita, M. Vershinin, S.P. Gross †+, and C.C.Yu+ , “Studying Molecular Motor-based Cargo Transport: What is Real, and What is Noise?”, Online Early Edition, Biophys. Jl, (2007)
39. J.E. Martinez, M.D. Vershinin , G.T. Shubeita, and S.P. Gross, “On the use of in vivo cargo velocity as a biophysical marker”, Biochem. Biophys. Res. Comm. 353, 835-840 (2007).
38. M. Vershinin, B.C. Carter, D.S. Razafsky, S.J. King and S.P. Gross, “Multiple-motor based transport and its regulation by Tau”, PNAS V. 104, 87–92 (2007). (track 2)
37. R. Mallik and S. P. Gross, “Molecular motors as cargo transporters in the cell —The good, the bad and the ugly”, Physica A, V. 372, 65 –69, (2006)
36. S. Cermelli*, Y. Guo, S.P. Gross+† and M.A. Welte+, "The Lipid-Droplet Proteome Reveals that Droplets Are a Protein-Storage Depot", Curr. Bio., v. 16, 1783-1795, (2006).
35. S. L. Bullock, A. Nicol, S.P. Gross, and D. Zicha, " Guidance of Bidirectional Motor Complexes by mRNA Cargoes through Control of Dynein Number and Activity", Curr. Bio.,V. 16, 1447–1452, (2006).
34. S.E. Antinone, G.T. Shubeita, K.E. Coller, J.I. Lee, S. Haverlock-Moyns, S.P. Gross+, and G.A. Smith+, "The herpesvirus capsid surface protein, VP26, and the majority of the tegument proteins are dispensable for capsid transport toward the nucleus", J. Virol., n. 80, 5494–5498 (2006)
33. Roop Mallik, Dmitri Petrov, S.A. Lex, S.J. King, and S.P. Gross, "Building Complexity: An In Vitro Study of Cytoplasmic Dynein with In Vivo Implications", Curr. Bio., v. 15, 2075-2085, (2005).
32. M.P. Singh, R. Mallik, S. P. Gross § , and C.C. Yu § , "Monte Carlo modeling of single molecule cytoplasmic dynein", PNAS, v. 102, 12059–12064, (2005). (§ =co-senior author)
31. M.A. Welte*, S. Cermelli*, J. Griner, A. Viera, Y. Guo, D. Kim, J.G. Gindhart , S.P. Gross, "Regulation of lipid-droplet transport by the Perilipin homologue LSD2", Curr. Bio., v. 15, 1266-1275, (2005).
30. F. Lin, CM Nguyen, SJ Wang, W Saadi, SP Gross, NL Jeon, "Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices", Ann. Biom. Engin., v. 33, no. 4, 475-482 (2005).
29. Brian C. Carter, George T. Shubeita, and Steven P. Gross, “ Tracking single-particles: a user-friendly quantitative evaluation", Physical Biology 2, 60–72, (2005).
28. T. del Rio, T.H. Ch’ng 2, E.A. Flood, S.P. Gross, and L.W. Enquist “ Heterogeneity of a fluorescent tegument component in single psedorabies virons and enveloped axonal assemblies", J. Virol. , n. 79, 3903-19 (2005).
27. Roop Mallik and Steven P. Gross, “Molecular Motors: Strategies to Get Along”, Current Biology, v. 14, R971-R982, (2004).
26. G.A. Smith, L. Pomeranz, S.P. Gross§ and L. Enquist§, “Local modulation of plus-end transport targets herpesvirus entry and egress in sensory axons", PNAS early eddition, (2004) (§ =co-senior author)
25. J. Snider, F. Lin, N. Zahedi, V. Rodionov, C.C. Yu§, and S.P. Gross§, “Intracellular actin-based transport: How far you go depends on how often you switch", PNAS 101: 13204–13209, (2004) (§ =co-senior author)
24. SP Gross “Hither and yon: a review of bi-directional microtubule-based transport", Physical Biology 1: R1–R11, (2004)
23. F. Lin, CM Nguyen, SJ Wang, W Saadi, SP Gross§, NL Jeon§, “Effective neutrophil chemotaxis is strongly influenced by mean IL-8 concentration”, Biochem. Biophys. Res. Commun. Jun 25;319(2):576-81 (2004). (§ =co-senior author)
22. R. Mallik, B.C. Carter, S.A. Lex, S.J. King and S.P. Gross “Cytoplasmic dynein functions as a gear in response to load”, Nature 427, 649-52 (2004).
21. Vladimir Rodionov, Julie Yi, Anna Kashina, Abiola Oladipo, and Steven P. Gross, “Switching between microtubule- and actin-based transport systems in melanophores is controlled by cAMP levels”, Current Biology, v. 13, 1837–1847, (2003).
20. Steven P. Gross,Yi Guo, Joel E. Martinez, and Michael A. Welte, “A Determinant for Directionality of Organelle Transport in Drosophila Embryos”, Current Biology, v. 13, 1660–1668, (2003).
19. S. P. Gross, “Dynactin: Coordinating Motors with Opposite Inclinations (Dispatch)”, Current Biology, v. 13, R320-322 (2003).
18. S. P. Gross, “Application of Optical Traps In Vivo”, Methods in Enzymology, v. 361, 162-174 (2003).
17. L.J. Davis, D.J. Odde, S. M. Block, and S. P. Gross, “The Importance of Lattice Defects in Katanin-Mediated Microtubule Severing in Vitro”, Biophys. J. 82, 2916-27 (2002).
16. S. P. Gross*, M. C. Tuma*, S. W. Deacon, A. S. Serpinskaya A. R. Reilein and V. I. Gelfand, “Interactions and Regulation of Molecular Motors in Xenopus Melanophores”, J. Cell Bio. 156, 855-65 (2002).
15. S.P Gross*, M. Welte*, S.M. Block, and E.F. Wieschaus, “Coordination of opposite-polarity microtubule motors”, J. Cell Bio. 156, 715-24, (2002)
14. L.W. Enquist, M.J. Tomishima, S. Gross, G.S. Smith, “Directional spread of an alpha-herpsesvirus in the nervous system”, Veter. Microb. 2266, 1-12 (2002).
13. G.A. Smith*, S.P Gross*, and L.W. Enquist, “Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons”, PNAS 98 3466-70 (2001)
12. S.P Gross, M. Welte, S.M. Block, and E.F. Wieschaus, “Dynein-mediated cargo transport In vivo. A switch controls travel distance.”, J. Cell Biol. 148 945-56 (2000).
11. S.P. Gross*, M. Welte*, M. Postner, S. M. Block, and E.F. Wieschaus, “Developmental and Genetic Regulation of Vesicle Transport in Drosphila Embryos”, Cell 92, 547 (1998).
10. K. Visscher, S. P. Gross, and S. M. Block, “Construction of Multiple-Beam Optical Traps with Nanometer-Resolution Position Sensing”, IEEE Jl. Sel. Top. Quant. Electr., 2, 1066 (1996).
9. E. Sharon, S. P. Gross, and J. Fineberg, “Energy Dissipation in Dynamic Fracture,” Phys. Rev. Lett., 76, 2117 (1996).
8. S.P. Gross, “Instabilities in Fast Fracture”, Ph.D. Dissertation, University of Texas, (1995)
7. E. Sharon*, S. P. Gross*, and J. Fineberg, ``Local Crack Branching as a Mechanism for Instability in Dynamic Fracture,'' Phys. Rev. Lett, 74, 5096 (1995).
6. M. Marder and Steve Gross, ``Origin of Crack Tip Instabilities,'' Jl. of the Mech. and Phys. of Sol., 43, 1 (1995).
5. S. P. Gross, J. Fineberg, M. Marder, W.D. McCormick, and H. L. Swinney, ``Acoustic Emissions from Rapidly Moving Cracks," Phys. Rev. Lett., 71, 3162 (1993).
4. J. Fineberg, S. P. Gross, M. Marder, and H. L. Swinney, ``Instability in the propagation of fast cracks'', Phys. Rev. B, 45, 5146, 1992.
3. J. Fineberg, S. P. Gross, M. Marder, and H. L. Swinney, ``Instability in Dynamic Fracture,'' Phys. Rev. Lett., 67, 457, (1991).
2. S. Gross, G. Zocchi, and A. Libchaber ``Waves and Plumes of thermal boundary layer,'' C. R. Acad. Sci. Paris, 307, Serie II, 447, (1988).
1. J. Glazier, S. P. Gross, J. Stavans, ``Dynamics of two-dimensional soap froths'', Phys. Rev. A, 36, 306, 1987.
American Society of Cell Biology