Home | For Faculty | Help | About | Webmaster

Rongsheng Jin

Associate Professor, Physiology & Biophysics
School of Medicine

Ph.D., Columbia University, 2003

Phone: (949) 824-6580
Email: r.jin@uci.edu

University of California, Irvine
Medical Sciences I, C-333
Mail Code: 4560
Irvine, CA 92697

picture of Rongsheng  Jin

Research
Interests
Structure and function of synaptic proteins; neurotoxins and receptors; protein complexes; protein-protein and protein-ligand interactions; X-ray crystallography
   
Academic
Distinctions
Alfred P. Sloan Research Fellowship, 2009

Young Investigators Grant, Human Frontier Science Program, 2011

Wonderful Original Work Award (Sanford-Burnham Medical Research Institute), 2012
   
Research
Abstract
Our research focuses on the structure and function of botulinum neurotoxins (BoNTs), which are among the most poisonous substances known to man. BoNTs therefore represent a major bioterrorist threat. Paradoxically, BoNT-containing medicines and cosmetics have been used with great success in clinic. Both the toxic and therapeutic functions of BoNTs indeed rely on a common mechanism to enter neurons, cleave proteins that mediate release of key neurotransmitters, and subsequently paralyze the affected muscles. We are trying to understand the molecular mechanisms underlying the BoNT-host interplay during the course of intoxication. These studies will guide the development of effective anti-BoNT strategies, will help improve clinical efficacy of BoNT-containing drugs, and will suggest novel applications for BoNT.

A second area of our research concerns the structural and functional characterization of ion channels, receptors, and signaling molecules in the nervous system. The brain is a massive network of electrically active cells (neurons) that communicate with each other by signal transmission at specialized intercellular junctions called synapses. We are interested in mechanisms of synapse formation, neurotransmission, and synaptic plasticity. These studies will facilitate the design and improvement of therapeutic agents for the treatment of psychological and neurological disorders.
   
Available Technologies
Publications Lee, K., Zhong, X., Gu, S., Kruel, A. M., Dorner, M. B., Perry, K., Rummel, A., Dong, M., and Jin, R. Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex. Science, 344(6190), 1405-10 (2014)
   
  Lee, K., Lam, K. H., Kruel, A. M., Perry, K., Rummel, A., and Jin, R. High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex. Biochem. Biophys. Res. Commun., 446(2), 568-573 (2014)
   
  Yao, Y., Lee, K., Gu, S., Lam, K. H., and Jin, R. Botulinum Neurotoxin A Complex Recognizes Host Carbohydrates through Its Hemagglutinin Component. Toxins (Basel), 6(2), 624-635 (2014)
   
  Lee, K., Gu, S., Jin, L., Le, T.T. N., Cheng, L. W., Strotmeier, J., Kruel, A. M., Yao, G., Perry, K., Rummel, A., and Jin, R. Structure of a Bimodular Botulinum Neurotoxin Complex Provides Insights into Its Oral Toxicity. PLoS Pathog, 9(10), e1003690 (2013)
   
  Gu, S. and Jin, R. Assembly and function of the botulinum neurotoxin progenitor complex. Current Topics in Microbiology and Immunology 364:21-44 (2013)

Zong, Y. and Jin, R. Structural mechanisms of the agrin-LRP4-MuSK signaling pathway in neuromuscular junction differentiation. Cellular and Molecular Life Sciences [Epub ahead of print] (2012)

Gu, S., Rumpel, S., Zhou, J., Strotmeier, J., Bigalke, H., Perry, K., Shoemaker, C.B., Rummel, A. & Jin, R. Botulinum neurotoxin is shielded by NTNHA in an interlocked complex. Science 335(6071):977-81 (2012)

Zong, Y., Zhang, B., Gu, S., Lee, K., Zhou, J., Yao, G., Figueiredo, D., Perry, K., Mei, L. & Jin, R. Structural basis of neuron-specific regulation of postsynaptic differentiation. Gene & Development 26:247-258 (2012)

Yao, G., Zong, Y., Gu, S., Zhou, J., Xu, H., Mathews, II. & Jin, R. Crystal structure of the glutamate receptor GluA1 amino-terminal domain. Biochemical Journal 438(2):255-63 (2011)

Strotmeier, J., Gu, S., Jutzi, S., Mahrhold, S., Zhou, J., Pich, A., Eichner, T., Bigalke, H., Rummel, A., Jin, R. & Binz, T. The biological activity of botulinum neurotoxin type C is dependent upon novel types of ganglioside binding sites. Mol. Microbiol. 81(1): 143-56 (2011)

Strotmeier, J., Lee, K., Völker, A.K., Mahrhold, S., Zong, Y., Zeiser, J., Zhou, J., Pich, A., Bigalke, H., Binz, T., Rummel, A. & Jin, R. Botulinum neurotoxin serotype D attacks neurons via two carbohydrate-binding sites in a ganglioside-dependent manner. Biochemical Journal 431(2):207-16 (2010)

Jin, R., Singh, S.K., Gu, S., Furukawa, H., Sobolevsky, A.I., Zhou, J., Jin, Y. & Gouaux E. Crystal structure and association behavior of the GluR2 amino-terminal domain. EMBO J 28(12):1812-23 (2009)

Kumar, J., Schuck. P., Jin, R. & Mayer, M.L. The N-terminal domain of GluR6-subtype glutamate receptor ion channels. Nat Struct Mol Biol 16(6):631-8 (2009)

Brunger, A.T., Jin, R. & Breidenbach, M.A. Highly specific interactions between botulinum neurotoxins and synaptic vesicle proteins. Cell Mol Life Sci 65(15), 2296-306 (2008)

Jin, R., Sikorra, S., Stegmann, C.M., Pich, A., Binz, T. & Brunger, A.T. Structural and biochemical studies of botulinum neurotoxin serotype C1 light chain protease: implications for dual substrate specificity. Biochemistry 46(37), 10685-93 (2007)

Jin, R., Rummel, A., Binz, T. & Brunger, A.T. Botulinum neurotoxin B recognizes its protein receptor with high affinity and specificity. Nature 444:1092-5 (2006)

Jin, R., Junutula, J.R., Matern, H.T., Ervin, K.E., Scheller, R.H. & Brunger, A.T. Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase. EMBO J. 24:2064-74 (2005)

Jin, R., Clark, S., Weeks, A.M., Dudman, J.T., Gouaux, E. & Partin, K.M. Mechanism of positive allosteric modulators acting on AMPA receptors. J. Neurosci. 25(39):9027-36 (2005)

Jin, R., Bank, T., Mayer, M. L., Traynelis, S. & Gouaux, E. Structural basis for partial agonist action at ionotropic glutamate receptors. Nat. Neurosci. 6(8):803-10 (2003)
   
Professional
Societies
Society for Neuroscience
American Crystallographic Association
Biophysical Society
The Protein Society
International Society for Toxinology
   
Graduate Programs Cellular and Molecular Biosciences

   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5958
   
Last updated 08/21/2014