Alexander D. Boiko

Assistant Professor, Molecular Biology and Biochemistry
School of Biological Sciences

B.S., University of Illinois at Chicago, Chicago IL, 1997, Biology


Ph.D., University of Illinois at Chicago, College of Medicine, Department of Molecular Genetics, Chicago IL, 2005, Molecular and Cancer Biology

Phone: (949) 824-9953
Email: aboiko@uci.edu

University of California, Irvine
Stem Cell Research Center
Sue & Bill Gross Hall; a CIRM Institute
845 Health Sciences Road
Mail Code: 3900
Irvine, CA 92697

picture of Alexander D. Boiko

Research
Interests
Cancer, Stem Cells, Tumor Initiating Cells, Melanoma, Neural Crest
   
Appointments Postdoctoral Scholar, Laboratory of Irving Weissman, Stanford University, Institute for Stem Cell Biology and Regenerative Medicine
   
Research
Abstract
Research in Lay Terms
Our Lab is interested in understanding cellular and molecular origins of melanoma initiation and clonal progression, as well as, to determine factors underlying aggressive metastatic properties of this disease.

Another major focus of the lab is to study tumor heterogeneity and branched evolution using RNA-Seq and Onco-Exome Capture Seq approaches of human clinical tumor samples. In addition, we are utilizing transgenic mouse models of cell lineage tracing to understand clonality and heterogeneity of skin tumors in response to UV exposure.

Research Focus
Dr. Boiko’s Lab focuses on discovering key molecular differences between tumor initiating and differentiated cell populations using human surgical melanoma samples. In addition, they are studying involvement of the immune system in control of disease progression and how to mediate its components to design more efficient therapeutic regimens.

Detail on Research
The goal of our research is to identify molecular factors and cell lineages of neural crest origin that contribute to melanoma initiation. We are using gene expression analysis to pinpoint signaling networks that are circumvented during normal development or regeneration of melanocytic lineages and result in malignant melanoma transformation. Using RNA inhibition and cDNA overexpression approaches we modulate expression of the candidate genes in target cell populations that are then assayed for their tumorigenic properties in-vitro and in-vivo. Dr. Boiko lab is also interested in understanding the role of macrophages in melanoma progression and metastasis. Currently we are developing tools that modulate macrophage activity and testing their effect in-vivo in the mouse xenotransplantation model of human metastatic disease.
   
Publications Ngo M, Han A, Lakatos A, Debashis S, Hachey SJ, Weiskopf K, Beck A, Weissman IL, Boiko AD.
Antibody Therapy Targeting CD47 and CD271 Effectively Suppresses Melanoma Metastasis
Cell Reports, 2016, Accepted
   
  Hachey SJ, Boiko AD.
Therapeutic Implications of Melanoma Heterogeneity.
Exp Dermatol. 2016 Apr 8. [Epub ahead of print]
   
  Dimov IK, Boiko AD.
Profiling Melanoma Heterogeneity Using Microwell RNA Cytometry.
Methods Mol Biol. 2016 Apr 16. [Epub ahead of print]
   
  Boiko AD
Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues
Methods Mol Biol. 2013;961:253-9.
   
  Razorenova OV, Finger EC, Colavitti R, Chernikova SB, Boiko AD, Chan CK, Krieg A, Bedogni B, LaGory E, Weissman IL, Broome-Powell M, Giaccia AJ.
VHL loss in renal cell carcinoma leads to up-regulation of CUB domain-containing protein 1 to stimulate PKC{delta}-driven migration.
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1931-6.
   
  Boiko AD, Razorenova OV, van de Rijn M, Swetter SM, Johnson DL, Ly DP, Butler PD, Yang GP, Joshua B, Kaplan MJ, Longaker MT, Weissman IL.
Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271.
Nature. 2010 Jul 1;466(7302):133-7.
   
  Boiko AD, Porteous S, Razorenova OV, Krivokrysenko VI, Williams BR, Gudkov AV.
A systematic search for downstream mediators of tumor suppressor function of p53 reveals a major role of BTG2 in suppression of Ras-induced transformation.
Genes Dev. 2006 Jan 15;20(2):236-52.
   
  Lu T, Burdelya LG, Swiatkowski SM, Boiko AD, Howe PH, Stark GR, Gudkov AV.
Secreted transforming growth factor beta2 activates NF-kappaB, blocks apoptosis, and is essential for the survival of some tumor cells.
Proc Natl Acad Sci U S A. 2004 May 4;101(18):7112-7.
   
  Zou X, Ray D, Aziyu A, Christov K, Boiko AD, Gudkov AV, Kiyokawa H.
Cdk4 disruption renders primary mouse cells resistant to oncogenic transformation, leading to Arf/p53-independent senescence.
Genes Dev. 2002 Nov 15;16(22):2923-34.
   
  Axenovich SA, Kazarov AR, Boiko AD, Armin G, Roninson IB, Gudkov AV.
Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response.
Cancer Res. 1998 Aug 1;58(15):3423-8.
   
Professional
Societies
American Association of Cancer Research
International Society of Stem Cell Research
   
Other Experience Postdoctoral Research Fellow
Stanford University 2006—2011

Graduate Programs Cancer Biology

Cell Biology

Cellular and Molecular Biosciences

Developmental Biology and Genetics

Mechanisms of Gene Expression

Stem Cell Biology

   
Research Centers Chao Family Comprehensive Cancer Center
   
Stem Cell Research Center
   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5951
   
Last updated 06/23/2016