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Jonathan Robert Todd Lakey

Director of Research, Surgery
School of Medicine

Ph.D., University of Alberta, Edmonton, Alberta, Canada, 1995, Experimental Surgery


M.S., University of Alberta, Edmonton, Alberta, Canada, 1990, Physiology


B.S., University of Alberta, Edmonton, Alberta, Canada, 1986, Zoology

Phone: (714) 456-5386
Fax: (714) 456-6377
Email: jlakey@uci.edu

University of California, Irvine
333 City Boulevard West, Suite 700
Mail Code: 5375
Orange, CA 92868

picture of Jonathan Robert Todd Lakey

   
Publications 1. Lakey JRT, Wang LCH, Rajotte RV. Optimal temperature in short-term hypothermic preservation of rat pancreas. Transplantation 51:977-981, 1991.

2. Fabian MC, Lakey JRT, Rajotte RV, Kneteman NM. Rapamycin prolongs murine islet allograft survival. Transplantation Proc. 24:2842, 1992.

3. Warnock GL, Ryan EA, Kneteman NM, Ao Z, Lakey JRT, Rajotte RV. The natural history of transplantation of insulin-producing pancreatic islets into type 1 diabetic patients. Transplantation Proc. 24:3058, 1992.

4. Ao Z, Matayoshi K, Yakimets W, Lakey JRT, Katyal D, Rajotte RV, Warnock GL. Comparison of Dextran and Ficoll density gradient purification for canine islets. Transplantation Proc. 24:2788, 1992.

5. Ao Z, Lakey JRT, Rajotte RV, Warnock GL. Collagenase digestion of canine pancreas by gentle automated dissociation in combination with ductal perfusion optimizes mass recovery of islets. Transplantation Proc. 24:2787, 1992.

6. Lakey JRT, Warnock GL, Seelis REA, Ao Z, Kneteman NM, Rajotte RV. A comparison of human islet purification with discontinuous gradients of Ficoll prepared with Euro-Collins or Medium 199 solution. Transplantation Proc. 24:2825, 1992.

7. Lakey JRT, Wang T, Warnock GL, Singh B, Rajotte RV. Prevention of recurrence of insulin-dependent diabetes mellitus in islet cell transplanted diabetic NOD mice using adjuvant therapy. Transplantation Proc. 24:2848, 1992.

8. Lakey JRT, Thomson ABR, Keelan M, Lopaschuk GL, Clandinin MT, Cheeseman CI, Rajotte RV. Dietary lipid content influences the clinical intestinal adaptive responses to islet cell transplantation in diabetic rats. Diab. Res. 19:149-164, 1992.

9. Seelis REA, Warnock GL, Kneteman NM, Lakey JRT, Rajotte RV. Optimierung der Isolierung humaner Inseln fuer die transplantation bei Typ-1-Diabetes (Optimization of the isolation of islets of Langerhans for transplantation in type-1-diabetes). Diabetes und Stoffwechsel 1:8-13, 1992.

10. Kneteman NM, Lakey JRT, Warnock GL, Rajotte, RV. Human islet isolation after prolonged cold storage. Diab. Nutr. Metabol. 5(Suppl.1):33-37, 1992.

11. Lakey JRT, Warnock GL, Seelis REA, Ao Z, Kneteman NM, Rajotte RV. Human islet purification with discontinuous density gradients following cold storage of the pancreas. Transplantation Proc. 25:957-958, 1993.

12. Warnock GL, Lakey JRT, Rajotte RV. Commentary. Perspectives on the International Islet Transplantation Registry. Cell Transplantation 2:303-305, 1993.

13. Lopaschuk GD, Lakey JRT, Barr R, Wambolt R, Thomson ABR, Clandinin MT, Rajotte RV. Islet transplantation improves glucose oxidation and mechanical function in diabetic rat hearts. Can. J. Physiol. Pharmacol. 71:896-903, 1993.

14. Yakimets W, Lakey JRT, Yatscoff RW, Katyal D, Ao Z, Finegood DT, Rajotte RV, Kneteman NM. Prolongation of canine pancreatic islet allograft survival with combined rapamycin and cyclosporine therapy at low doses. Rapamycin efficacy is blood level related. Transplantation 56:1293-1298, 1993.

15. Fabian MC, Lakey JRT, Rajotte RV, Kneteman NM. The efficacy and toxicity of rapamycin in murine islet transplantation: In vitro and in vivo studies. Transplantation 56:1137-1142, 1993.

16. Ao Z, Matayoshi K, Lakey JRT, Rajotte RV, Warnock GL. Survival and function of purified islets in the omental pouch site of outbred dogs. Transplantation 56:524-529, 1993.

17. Taylor GD, Kirkland T, Lakey JRT, Rajotte RV, Warnock GL. Bacteremia due to transplantation of contaminated cryopreserved pancreatic islets. Cell Transplantation 3:103 106, 1994.

18. Glatz JFC, van Breda E, Keizer HA, De Jong YF, Lakey JRT, Rajotte RV, Thomson ABR, van der Vusse GJ, Lopaschuk GD. Rat heart fatty acid-binding protein content is increased in experimental diabetes. Biochem. Biophys. Res. Comm. 199:639-646, 1994.

19. Rabinovitch A, Suarez-Pinzon WL, Strynadka K, Schulz R, Lakey JRT, Warnock GL, Rajotte RV. Human pancreatic islet beta-cell destruction by cytokines is independent of nitric oxide production. J. Clin. Endocrinol. Metabol. 79:1058-1062, 1994.

20. Lakey JRT, Kizilisik TA, Rajotte RV, Ao Z, Warnock GL, Kneteman NM. Prospective evaluation of two techniques for human pancreas recovery before islet isolation. Transplantation Proc. 26(2):589, April 1994.

21. Lakey JRT, Warnock GL, Singh B, Rajotte RV. Adjuvant immunotherapy with Bacillus Calmette-Guerin prevents recurrence of diabetes in islet grafts transplanted into NOD mice. Transplantation Proc. 26(2):736, April 1994.

22. Lakey JRT, Warnock GL, Kneteman NM, Ao Z, Rajotte RV. Effects of pre-cryopreservation culture on human islet recovery and in vitro function. Transplantation Proc. 26(2):820, April 1994.

23. Lakey JRT, Rajotte RV, Taylor GD, Kirkland T, Warnock GL. Microbial studies of a tissue bank of cryopreserved human islet cells. Transplantation Proc. 26(2):827, April 1994.

24. Lakey JRT, Singh B, Warnock GL, Rajotte RV. BCG immunotherapy prevents recurrence of diabetes in islet grafts transplanted into spontaneously diabetic NOD mice. Transplantation 57:1213-1217, April 1994.

25. Kneteman NM, Lakey JRT, Kizilisik TA, Ao Z, Warnock GL, Rajotte RV. Cadaver pancreas recovery technique. Impact on islet recovery and in vitro function. Transplantation 58: 1114-1119, Nov 1994.

26. Lakey JRT, Warnock GL, Kneteman NM, Ao Z, Lakey JRT, Warnock GL, Kneteman NM, Ao Z, Rajotte RV. Development of a method for bulk cryopreservation of purified canine islets. Transplantation Proc. 26(6): 3300, Dec 1994.

27. Lakey JRT, Warnock GL, Rajotte RV. Conditions for the induction of unresponsiveness to cryopreserved islet allografts with intrathymic islets. Transplantation Proc. 26(6): 3313, Dec 1994.

28. Lakey JRT, Rajotte RV, Warnock GL, Kneteman NM. Cold ischemic tolerance of human pancreas: assessment of islet recovery and function. Transplantation Proc. 26(6):3416, Dec 1994.

29. Warnock GL, Lakey JRT, Ao Z, Rajotte RV. Tissue banking of cryopreserved islets for clinical islet transplantation. Transplantation Proc. 26(6): 3438, Dec 1994.

30. Gainer AL, Lakey JRT, Rajotte RV, Warnock GL. Survival and function of human islets subjected to Gamma radiation. Transplantation Proc. 26(6): 3457, Dec 1994.

31. Lakey JRT, Rajotte RV, Warnock GL. Microbial surveillance of human islet isolation, in vitro culture and cryopreservation. Clin. Invest. Med. 18:168-176, Feb 1995.

32. Lakey JRT, Rajotte RV, Warnock GL, Kneteman NM. Human pancreas preservation prior to islet isolation. Cold ischemic tolerance. Transplantation 59:689-694, Mar 1995.

33. Shapiro AMJ, Lakey JRT, Rajotte RV, Warnock GL, Friedlich M, Jewell L, Kneteman NM. Portal vein thrombosis after transplantation of partially purified pancreatic islets in a combined human liver-islet allograft. Transplantation 59:1060-1063, Apr 1995.

34. Lakey JRT, Singh B, Warnock GL, Elliott JF, Rajotte RV. Long-term survival of syngeneic islet grafts in BCG-treated diabetic NOD mice can be reversed by cyclophosphamide. Transplantation 59:1751-1753, Jun 1995.

35. Warnock GL, Katyal D, Okamura J, Lakey JRT, Rajotte RV. Studies of the isolation, viability, and preservation of purified islets after surgical pancreatectomy in large pigs. Xenotransplantation 2:161-164, 1995.

36. Warnock GL, Tsapogas P, Ryan E, Lakey JRT, Korbutt G, Kneteman NM, Ao Z, Rabinovitch A, Rajotte RV. Natural history of insulin independence after transplantation of multidonor cryopreserved pancreatic islets in type 1 diabetic humans. Transplantation Proc. 27:3159-3160, Dec 1995.

37. Kneteman NM, Lakey JRT, Wagner T, Finegood D. Beneficial metabolic impact of the novel immunosuppressant rapamycin in chronic canine islet allograft recipients. Transplantation Proc. 27:3213, Dec 1995.

38. Lakey JRT, Warnock GL, Rajotte RV, Ao Z, Suarez-Almazor ME, Shapiro AMJ, Kneteman NM. Factors in cadaveric donors that affect recovery of human islets of Langerhans. Transplantation Proc. 27:3265, Dec 1995.

39. Lakey JRT, Ao Z, Warnock GL, Korbutt GS, Flashner M, Colby CB, Rajotte RV. Recovery and in vivo function of canine islets cryopreserved in a freezer bag. Transplantation Proc. 27:3266, Dec 1995.

40. Cattral MS, Lakey JRT, Warnock GL, Kneteman NM, Rajotte RV. Effect of cryopreservation on islet recovery and function in vivo. Transplantation Proc. 27:3336, Dec 1995.

41. Rajotte RV, Ao Z, Korbutt GS, Lakey JRT, Flashner M, Colby CB, Warnock GL. Cryopreservation of microencapsulated canine islets. Transplantation Proc. 27:3389, Dec 1995.

42. Lakey JRT, Singh B, Warnock GL, Elliott JF, Rajotte RV. Dose dependence of Bacillus Calmette-Guerin on the prevention of autoimmune diabetes in syngeneic islet transplantation. Transplantation Proc. 27:3413, Dec 1995.

43. Tuitoek P, Lakey JRT, Rajotte RV, Ziari S, Tsin A, Basu T. Effect of insulin treatment or zinc supplementation on vitamin A status in streptozotocin-induced diabetic rats. J. Clin. Biochem. Nutr. 19:165-173, 1995.

44. Langman LJ, Shapiro AMJ, Lakey JRT, Legatt DF, Kneteman NM, Yatscoff RW. Pharmacodynamic assessment of mycophenolic acid induced immunosuppression by measurement of inosine monophosphate dehydrogenase activity in a canine model. Transplantation 61:87-92, Jan 1996.

45. Lakey JRT, Warnock GL, Rajotte RV. Intrathymic transplantation of fresh and cryopreserved islets for the induction of a state of unresponsiveness in rats. Transplantation 61:506-508, Feb 1996.

46. Tuitoek PJ, Lakey JRT, Rajotte RV, Basu TK. Strain variation in vitamin A (retinol) status of streptozotocin-induced diabetic rats. Int. J. Vit. Nutr. Res. 66:101-105, 1996.

47. Lakey JRT, Warnock GL, Ao Z, Rajotte RV. Bulk cryopreservation of isolated islets of Langerhans. Cell Transplantation 5:395-404, 1996.

48. Lakey JRT, Yatscoff RW, Koshal A, Rajotte RV. Prolongation of concordant islet xenograft with Leflunomide. Transplantation Proc. 28:816, Apr 1996.

49. Langman LJ, Shapiro AM, Lakey JRT, Legatt DF, Kneteman NM, Yatscoff RW. Pharmodynamic assessment of mycophenolic acid in a canine model. Transplantation Proc. 28:934-936, Apr 1996.

50. Lakey JRT, Warnock GL, Rajotte RV, Suarez-Almazor ME, Ao Z, Shapiro AMJ, Kneteman NM. Variables in organ donors that affect the recovery of human islets of Langerhans. Transplantation 61:1047-1053, Apr 1996.

51. Kneteman NM, Lakey JRT, Wagner T, Finegood D. The metabolic impact of rapamycin (Sirolimus) in chronic canine islet graft recipients. Transplantation 61:1206-1210, Apr 1996.

52. Rabinovitch A, Suarez-Pinzon WL, Strynadka K, Lakey JRT, Rajotte RV. Human pancreatic islet beta-cell destruction by cytokines involves oxygen free radicals and aldehyde production. J. Clin. Endocrinol. Metabol. 81:3197-3202, 1996.

53. Lakey JRT, Zieger MAJ, Woods E, Liu J, Critser JK. Hyposmotic exposure of pancreatic digest as a means to purify islet tissue. Cell Transplantation 6:423-428, 1997.

54. Liu J, Zieger MAJ, Lakey JRT, Woods E, Critser JK. The determination of membrane permeability coefficients of canine pancreatic islet cells and their application to islet cryopreservation. Cryobiology 35:1-13, 1997.

55. Lakey JRT, Warnock GL, Brierton M, Ao Z, Hering BJ, London NJM, Ricordi C, Corbin F, Rajotte RV. Development of an automated computer controlled islet isolation system. Cell Transplantation 6:47-57, 1997.

56. Gunasena KT, Lakey JRT, Villines PV, Critser ES, Critser JK. Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice. Biol. Reprod. 57:226-231, 1997.

57. Woods EJ, Zieger MAJ, Lakey JRT, Liu J, Critser JK. Osmotic characteristics of isolated human and canine pancreatic islets. Cryobiology 35:106-113, 1997.

58. de Freitas RC, Diller KR, Lakey JRT, Rajotte RV. Osmotic behavior and transport properties of human islets in a dimethyl sulfoxide solution. Cryobiology 35:230-239, 1997.

59. Gorski W, Aspinwall C, Lakey JRT, Kennedy RT. Ruthenium catalyst for amperometric determination of insulin at physiological pH. J. Electroanalyt. Chem. 425:191-199, 1997.

60. Lakey JRT, Warnock GL, Rajotte RV. Comparison of methods used for the removal of DMSO following islet cryopreservation and the development of an automated protocol. Cell Transplantation 6:163-172, 1997.

61. Aspinwall CA, Brooks SA, Kennedy RT, Lakey JRT. Effects of intravesicular H+ and Zn2+ on insulin secretion in pancreatic beta cells. J. Biol. Chem. 272:31308-31314, 1997.

62. Lakey JRT, Warnock GL, Rajotte RV. Optimization of the removal of cryoprotectant from frozen-thawed islets. Transplantation Proc. 29:1986, 1997.

63. Cavanagh T, Lakey JRT, Wright MJ, Fetterhoff T, Wile K. Crude collagenase loses islet-isolating efficacy regardless of storage conditions. Transplantation Proc. 29:1942, 1997.

64. Lakey JRT, Warnock GL, Brierton M, Ao Z, Hering BJ, London NJM, Ricordi C, Corbin F, Rajotte RV. Development of an automated computer-controlled islet isolation system. Transplantation Proc. 29:1956, 1997.

65. Liu J, Zieger MAJ, Lakey JRT, Woods EJ, Critser JK. Water and DMSO permeability at 22°C, 5°C, and -3°C for human pancreatic islet cells. Transplantation Proc. 29:1987, 1997.

66. Woods EJ, Lakey JRT, Zieger MAJ, Liu J, Nelson D, Critser JK. Permeability characteristics of microencapsulated pancreatic islets. Transplantation Proceedings. 29:2148, 1997.

67. Lakey JRT, Cavanagh T, Zieger MAJ, Wright MJ. Evaluation of purified enzyme blend for the recovery and function of canine pancreatic islets. Cell Transplantation 7:365-372, 1998.

68. Cattral MS, Lakey JRT, Warnock GL, Kneteman NM, Rajotte RV. Effect of cryopreservation on the survival and function of murine islet isografts and allografts. Cell Transplantation 7:373-379, 1998.

69. Lakey JRT, Cavanagh TJ, Zieger MAJ. A prospective comparison of discontinuous euroficoll and eurodextran gradients for islet purification. Cell Transplantation 7:479-487, 1998.

70. Qin HY, Elliott JF, Lakey JRT, Rajotte RV, Singh B. Endogenous immune response to Glutamic Acid Decarboxylase (GAD 67) in NOD mice are modulated by adjuvant immunotherapy. J. Autoimmunity 11:591-601, 1998.

71. Gunasena KT, Lakey JRT, Villines PV, McGann LE, Bush M, Raath C, Critser ES, Critser JK. Antral follicles in xenogeneic cryopreserved elephant (Loxodonta Africana) ovarian tissue. Animal Reproduction Science 53:265-275, 1998.

72. Lakey JRT, Smith ME, Fetterhoff TJ, Cavanagh TJ, Wright MJ, Dwulet FE. Sterile filtration of collagenase solution: effect of filter membrane composition on enzyme recovery. Transplantation Proc. 30:347, 1998.

73. Lakey JRT, Cavanagh TJ, Zieger MA, Critser JK. A prospective comparison of discontinuous EuroFicoll and EuroDextran gradients for islet purification. Transplantation Proc. 30:348, 1998.

74. Lakey JRT, Warnock GL, Ao Z, Shapiro AMJ, Korbutt G, Kneteman NM, Rajotte RV. Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion. Transplantation Proc. 30:359, 1998.

75. Cavanagh TJ, Lakey JRT, Dwulet F, Wright MJ, Wile K, Albertson TA, Fetterhoff TJ. Improved pig islet yield and post-culture recovery using Liberase PI purified enzyme blend. Transplantation Proc. 30:367, 1998.

76. Cavanagh TJ, Lakey JRT, Wright MJ, Albertson T, Fetterhoff TJ. Identification of a pig strain with maximal islet mass. Transplantation Proc. 30:368, 1998.

77. Zieger MA, Lakey JRT. Islet cryopreservation strategies: Maintaining islet volume within tolerable limits during freezing. Transplantation Proc. 30:381, 1998.

78. Lakey JRT, Aspinwall CA, Cavanagh TJ, Kennedy RT. Effect of cryopreservation on canine islet insulin secretion as measured by amperometric techniques. Transplantation Proc. 30:382, 1998.

79. Lakey JRT, Troendle J, Zieger MA, Geary WA, Voytek S, Critser JK. Improved islet survival and in vitro function using small intestinal submucosa. Transplantation Proc. 30:383, 1998.

80. Woods EJ, Lakey JRT, Zieger MA, Liu J, Critser JK. Osmotic responses of isolated canine islets to novel cryoprotectants. Transplantation Proc. 30:388-389, 1998.

81. Rilo HLR, Paljug WR, Lakey JRT, Taylor MJ, Grayson D. Biolistic engineering of pancreatic beta-cells with fluorescent green protein. Transplantation Proc. 30:465-468, 1998.

82. Lakey JRT, Pardue D, Calvin S, Albertson T, Cavanagh TJ, Jacobsen L. Nonviral transfection of isolated islets of Langerhans. Transplantation Proc. 30:469, 1998.

83. Shapiro AMJ, Hao E, Lakey JRT, Finegood D, Rajotte RV, Kneteman NM. Diabetogenic synergism in canine islet autografts from cyclosporine and steroids in combination. Transplantation Proc. 30:527, 1998.

84. Lakey JRT, Cavanagh TJ, Zieger MAJ, Albertson TA, Dwulet F, Wright MJ, Fetterhoff TJ. Evaluation of a purified enzyme blend for the recovery and in vitro function of isolated canine islets. Transplantation Proc. 30:590-591, 1998.

85. Shapiro AMJ, Hao E, Lakey JRT, Elliott J, Rajotte RV, Kneteman NM. Development of diagnostic markers for islet allograft rejection. Transplantation Proc. 30:647, 1998.

86. Lakey JRT. Examination of microbiological contamination rates following bacterial culture and incubation for 2, 7, and 14 days. Transplantation Proc. 30:664, 1998.

87. Lakey JRT, Zieger MA, Woods EJ, Critser JK, Wright JR, Jr. Permeability characteristics of isolated Brockmann Bodies. Transplantation Proc. 30:380, 1998.

88. Woods EJ, Liu J, Zieger MAJ, Lakey JRT, Critser JK. Water and cryoprotectant permeability characteristics of isolated human and canine pancreatic islets. Cell Transplantation 8:549-559, 1999.

89. Lakey JRT, Aspinwall CA, Cavanagh TJ, Kennedy RT. Secretion from islets and single islet cells following cryopreservation. Cell Transplantation 8:691-698, 1999.

90. Woods EJ, Liu J, Zieger MAJ, Lakey JRT, Critser JK. The effects of microencapsulation on pancreatic islet osmotically induced volumetric response. Cell Transplantation 8:699-708, 1999.

91. Paraskevas S, Aikin R, Maysinger D, Lakey JR, Cavanagh TJ, Hering B, Wang R, Rosenberg L. Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: implications for cultured islet survival. FEBS Lett. 455:203-208, 1999.

92. Warnock GL, Lakey JRT. Cryopreservation of human islets of Langerhans. Transplantation 68:597-598, 1999.

93. Aspinwall CA, Huang L, Lakey JRT, Kennedy RT. Comparison of amperometric methods for detection of exocytosis from single pancreatic B cells from different species. Analytical Chemistry 71:5551-5556, 1999.

94. Zieger MAJ, Woods EJ, Lakey JRT, Liu J, Critser JK. Osmotic tolerance limits of canine pancreatic islets. Cell Transplantation 8:277-284, 1999.

95. Lakey JRT, Warnock GL, Shapiro AMJ, Korbutt GS, Ao Z, Kneteman NM, Rajotte RV. Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion. Cell Transplantation 8:285-292, 1999.

96. Aspinwall CA, Lakey JRT, Kennedy RT. Insulin-stimulated insulin secretion in single pancreatic beta cells. J. Biol. Chem. 274(10):6360-6365, 1999.

97. Korbutt GS Lakey JRT. Seeking new sources: researchers look for a solution to the. Diabetes Dialogue, Winter Issue: 12-13, 1999.

98. Paras CD, Qian W, Lakey JRT, Tan WH, Kennedy RT. Localized exocytosis detected by spatially-resolved amperometry at single pancreatic ?-cells. Cell Biochem. Biophys. 33:227-240, 2000.

99. Shapiro AMJ, Lakey JRT, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. New England J. of Medicine 343:230 238, 2000.

100. Shapiro AMJ, Lakey JRT. Future trends in islet cell transplantation. Diabetes Technology and Therapeutics 2(3):449-52, 2000.

101. Shapiro AMJ, Lakey JRT. Clinical islet transplantation: into the new millennium. Canadian Journal of Diabetes Care 24(3):41-46, 2000.

102. Shapiro AMJ, Lakey JRT. Recent update and future directions in islet transplantation. Canadian Journal of Diabetes Care 24(3):44-46, 2000.

103. Paraskevas S, Aikin R, Maysinger D, Lakey JRT, Cavanagh TJ, Agapitos D, Wang R, Rosenberg L. Modulation of JNK and p38 stress activated protein kinases in isolated islets of Langerhans: Insulin as an autocrine survival signal. Ann. Surgery 233:124-133, 2001.

104. Ryan EA, Lakey JRT, Rajotte RV, Korbutt GS, Kin T, Imes S, Rabinovitch A, Elliott JF, Bigam D, Kneteman NM, Warnock GL, Larsen I, Shapiro AMJ. Clinical outcomes and insulin secretion after islet transplantation with the Edmonton Protocol. Diabetes 50:710-719, Apr 2001.

105. Shapiro AMJ, Ryan EA, Warnock GL, Kneteman NM, Korbutt GS, Rajotte RV, Lakey JRT. Could fewer islet cells be transplanted in type 1 diabetes? Insulin independence should be dominant force in islet transplantation. British Medical Journal Letters 322:861, Apr 2001.

106. Lakey JRT, Rajotte RV, Fedorow CA, Taylor MJ. Islet cryopreservation using intracellular preservation solutions. Cell Transplantation 10:533-589, 2001.

107. Fedorow C, McGann LE, Korbutt GS, Rayat GR, Rajotte RV, Lakey JRT. Osmotic and cryoprotectant permeation characteristics of islet cells isolated from the newborn pig pancreas. Cell Transplantation 10:651-659, 2001.

108. Shapiro AMJ, Hao EG, Lakey JRT, Yakimets WJ, Churchill TA, Mitlianga PG, Papadopoulos GK, Elliott JF, Rajotte RV, Kneteman NM. Novel approaches toward early diagnosis of islet allograft rejection. Transplantation 71:1709-1718, Jun 2001.

109. Lakey JRT, Anderson TJ, Rajotte RV. Novel approaches to cryopreservation of human pancreatic islets. Transplantation 72:1005-11, Jun 2001.

110. Lakey JRT, Young ATL, Pardue D, Calvin S, Albertson TE, Jacobson L, Cavanagh TJ. Nonviral transfection of intact pancreatic islets. Cell Transplantation 10:697-708, 2001.

111. Mercer DF, Schiller DE, Elliott JF, Douglas DN, Hao C, Rinfret A, Addison WR, Fischer KP, Churchill TA, Lakey JRT, Tyrrell DLJ, Kneteman NM. Hepatitis C virus replication in mice with chimeric human livers. Nature Medicine 7:927-933, Aug 2001.

112. Lakey JRT, Helms LMH, Kin T, Korbutt GS, Rajotte RV, Shapiro AMJ, Warnock GL. Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia. Transplantation 72:565-570, Aug 2001.

113. Storrs R, Dorian R, King SR, Lakey JRT, Rilo H. Preclinical development of the islet sheet. Ann. of the New York Academy of Sciences 944:252-266, Nov 2001.

114. Ryan EA, Lakey JRT, Shapiro AMJ. Clinical results after islet transplantation. J of Investigative Medicine 49(Suppl):559-62, 2001.

115. Matsumoto S, Lawrence O, Rigley TH, Lakey JRT, Stevens RB, Strong DM. University of Wisconsin. Solution with trypsin inhibitor Pefabloc improves survival of viable human and primate impure islets during storage. Cell & Tissue Banking 2:15-21, 2001.

116. Lakey JRT, Suarez-Pinzon WL, Strynadka K, Korbutt GS, Rajotte RV, Mabley JG, Szabo C, Rabinovitch A. Peroxynitrite is a mediator of cytokine-induced destruction of human pancreatic islet. Laboratory Investigation 81:1683-1692, Dec 2001.

117. Shapiro AMJ, Ryan EA, Lakey JRT. Islet cell transplantation. The Lancet (Supplement) 358: S21, Dec 2001.

118. Ricordi C, Lakey JRT, Hering BJ. Challenges toward standardization of islet isolation technology. Transplantation Proc. 33:1709, 2001.

119. Shapiro AMJ, Ryan EA, Lakey JRT. Clinical islet transplant – state of the art. Transplantation. Proc. 33:3502-3503, 2001.

120. Paty BW, Shapiro AMJ, Lakey JRT, Ryan EA. The Edmonton protocol for islet transplantation: Indications and outcomes. Canadian Diabetes 14:3-7, 2001.

121. Shapiro AMJ, Lakey JRT. Islet Transplantation. Promising diabetes therapy being tested in ten centers. Transplant Team 3:4-5 (Focal Point), 2001.

122. Lakey JRT, Woods EJ, Zieger MAJ, Avila JG, Geary WA, Voytik-Harbin SL, Critser JK. Improved islet survival and in vitro function using solubilized small intestinal submucosa. Cell & Tissue Banking 2:217-224, 2001.

123. Young ATL, Lakey JRT, Murray A, Moore RB. Gene therapy: A lipofection approach for gene transfer into primary endothelial cells. Cell Transplantation 11:573-582, 2002.

124. Lakey JRT, Kneteman NM, Rajotte RV, Wu DC, Bigam D, Shapiro AMJ. Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability. Transplantation 73:1106-1110, Apr 2002.

125. Lakey JRT, Tsujimura T, Shapiro AMJ, Kuroda Y. Human pancreas preservation prior to islet isolation. Cell Preservation Tech. 1:81-87, 2002.

126. Burridge PW, Shapiro AMJ, Ryan EA, Lakey JRT. Future trends in clinical islet transplantation. Transplant. Proc. 34:3347-3348, 2002.

127. Shapiro AMJ, Ryan E, Rajotte RV, Korbutt G, Kneteman NM, Bigam D, Paty B, Lakey JRT. Improved function of islet grafts under steroid-free immunosuppression – will grafts work forever? Response to letter by Birkeland et al, 2001. Transplantation 73:1528-1529, May 2002.

128. Geisler JG, Zawalich W, Zawalich K, Lakey JRT, Stukenbrok H, Milici AJ, Soeller WC. Estrogen can prevent or reverse obesity and diabetes in mice expressing human islet amyloid polypeptide. Diabetes 51:2158-2169, Jul 2002.

129. Lakey JRT, Burridge PW, Rajotte RV. Cryopreservation of human pancreatic islets. Graft 5:266-276, Jul/Aug 2002.

130. Ryan EA, Lakey JRT, Paty BW, Imes S, Korbutt GS, Kneteman NM, Bigam D, Rajotte RV, Shapiro AMJ. Successful islet transplantation: Continued insulin reserve provides long-term glycemic control. Diabetes 51:2148-2157, Jul 2002.

131. Casey JJ, Lakey JRT, Ryan EA, Paty B, O’Kelly K, Bigham D, Kneteman NM, Shapiro AMJ. Portal venous pressure changes following sequential clinical islet transplantation. Transplantation 74: 913-915, Oct 2002.

132. MacDonald PE, Wang G, Tsak S, Dodo C, Kang Y, Tang L, Wheeler MB, Cattral M, Lakey JRT, Salapatek AMF, Lotan I, Gaisano HY. Synaptosome-associated protein of 25 kilodaltons modulates Kv2.1 voltage dependent K(+) channels in neuroendocrine islet beta-cells through an interaction with the channel N terminus. Molecular Endocrinology 16:2452-2461, Nov 2002.

133. Shapiro AMJ, Hao EG, Lakey JRT, Finegood DT, Rajotte RV, Kneteman NM. Defining optimal immunisuppression for islet transplantation based on reduced diabetogenicity in canine islet autografts. Transplantation 74:1522-1529, Dec 2002.

134. Thomas D, Yang H, Boffa DJ, Ding R, Sharma VK, Langman M, Li B, Hering B, Mohanakumar T, Lakey JRT, Kapur S, Hancock WW, Suthanthiran M. Proapoptotic bax is hyperexpressed in isolated human islets compared with antiapoptotic BCL-2. Transplantation 74:1489-1496, Dec 2002.

135. Beattie GM, Montgomery AM, Lopez AD, Hao E, Perez B, Just ML, Lakey JRT, Hart ME, Hayek A. A novel approach to increase human islet cell mass while preserving beta-cell function. Diabetes, 51: 3435-3439, Dec 2002.

136. Paty BW, Ryan EA, Shapiro AMJ, Lakey JRT, Robertson RP. Intrahepatic islet transplantation in type 1 diabetic patients does not restore hypoglycemic hormonal counter-regulation or symptom recognition after insulin independence. Diabetes 51:3428-3434, Dec 2002.

137. Rose NL, Palcic MM, Lakey JRT. Evaluating the effect of serine proteases on Collagenase activity during human islet isolation. Cell Transplantation 11:821-826, 2002.

138. Lu W-T, Lakey JRT, Juang J-H, Hsu BR-S, Rajotte RV. Effect of pefabloc on islet isolation from cold preserved rat pancreas. Transplantation Proc. 34:2700-2701, 2002.

139. Lakey JRT, Tsujimura T, Shapiro AMJ, Kuroda Y. Preservation of the human pancreas before islet isolation using a two-layer (UW solution/perfluorochemical) cold storage method. Transplantation 74:1809-1811, Dec 2002.

140. Tsujimura T, Kuroda Y, Kin T, Avila JG, Rajotte RV, Korbutt GS, Ryan EA, Shapiro AMJ, Lakey JRT. Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW/solution/perfluorochemical) cold storage method. Transplantation 74:1687-1691, Dec 2002.

141. Burridge PW, Shapiro AMJ, Ryan EA, Lakey JRT. Islet transplantation: From bench to bedside and beyond. The Monitor, Newsletter of Diabetes Care Program 3:1, 2002.

142. Hathout E, Lakey JRT, Shapiro AMJ. Islet cell transplant – an option for childhood diabetes? Archive of Disease in Children 88:591-594, 2003.

143. Kobayashi N, Okitsu T, Nishimura W, Tanaka N, Lakey JRT. Islet transplantation at the University of Alberta. Japanese Journal of Regenerative Medicine 2:80-83, Feb 2003.

144. Lu WT, Lakey JRT, Juang JH, Hsu BR, Rajotte RV. Effect of trypsin inhibitor on islet isolation from fresh and cold preserved rat pancreases. Transplantation Proc. 35:488-489, 2003.

145. Rose NL, Palcic MM, Helms LMH, Lakey JRT. Evaluation of pefabloc as a serine protease inhibitor during human islet isolation. Transplantation 75:462-466, 2003.

146. Rose NL, Palcic MM, Lakey JRT. An evaluation of endogenous pancreatic enzyme levels after human islet isolation. Pancreas 27:167-173, 2003.

147. Shapiro AMJ, Gallant HL, Hao EG, Lakey JRT, McCready T, Rajotte RV, Yatscoff RW, Kneteman NM. The portal immunosuppressive storm - relevance to islet transplantation? Therapeutic Drug Monitoring, 27:35-37, Feb 2003.

148. Shapiro AMJ, Lakey JRT. Islet transplant - A secure future for cellular replacement therapy in diabetes. Practical Diab. Int. 20:145-149, May 2003.

149. Zhu JZJ, Castillo EG, Salehi P, Avila J, Lakey JRT, Churchill TA. A novel technique of hypothermic luminal perfusion for small bowel preservation. Transplantation 76:71-76, Jul 2003.

150. Avila JG, Tsujimura T, Oberholzer J, Churchill T, Salehi P, Shapiro AMJ, Lakey JRT. Improvement of pancreatic islet isolation outcomes using glutamine perfusion during isolation procedure. Cell Transplantation 12:877-881, 2003.

151. Burridge PW, Shapiro AMJ, Ryan EA, Lakey JRT. Clinical islet transplantation in the new millennium. The Journal of Egyptian Society of Endocrinology, Metabolism and Diabetes, 35:5-10, Jul 2003.

152. Lakey JRT, Burridge PW, Shapiro AMJ. Technical aspects of islet preparation and transplantation. Transplantation International 16:613-632, 2003.

153. Salehi P, Zhu JZJ, Castillo EG, Avila JG, Lakey JRT, Churchill TA. Preserving the mucosal barrier during small bowel storage. Transplantation 76:911-917, Sep 2003.

154. Owen RJ, Ryan EA, O’Kelly K, Lakey JRT, McCarthy MC, Paty BW, Bigam DL, Kneteman NM, Korbutt GS, Rajotte RV, Shapiro AMJ. Percutaneous transhepatic pancreatic islet cell transplantation in type 1 diabetes mellitus: radiologic aspects. Radiology 229:165-170, Oct 2003.

155. Lakey JRT. Isolating islets: State of the art. Insulin-Free Times. Online Newsletter, Fall 2003.

156. Shapiro AM, Nanji SA, Lakey JRT. Clinical islet transplant: current and future directions towards tolerance. Immunological Reviews 196:219-236, 2003.

157. Woods EJ, Liu J, Zieger AJ, Lakey JRT, Critser JK. A theoretical examination of the biophysical factors for development of an optimized cryopreservation procedure for canine islets. Cell Preservation Tech. 1:151-164, Nov 2003.

158. Lakey JRT, Helms LMH, Moser G, Lix B, Slupsky CM, Rebeyka IM, Sykes BD, McGann LE. Dynamics of cryoprotectant permeation in porcine heart valve leaflets. Cell Transplantation 12:123-128, 2003.

159. Rose NL, Palcic MM, Lakey JRT. An evaluation of endogenous pancreatic enzyme levels following enzymatic digestion. Transplantation Proc. 35:2458-2460, Nov 2003.

160. Tsujimura T, Kuroda Y, Avila JG, Kin T, Churchill TA, Shapiro AMJ, Lakey JRT. Resuscitation of the ischemically damaged human pancreas by the two-layer method prior to islet isolation. Transplantation Proc. 35:2461-2462, Nov 2003.

161. Rose NL, Palcic MM, Lakey JRT. An evaluation of the activation of endogenous pancreatic enzymes during human islet isolations. Transplantation Proc. 35:2455-2457, Nov 2003.

162. Young AT, Lakey JRT, Murray AG, Mullen JC, Moore RB. In vitro senescence occurring in normal human endothelial cells can be rescued by ectopic telomerase activity. Transplantation Proc. 35:2483-2485, Nov 2003.

163. Raphael E, Ryan EA, Paty BW, Oberholzer J, Imes S, Senior P, McDonald C, Lakey JRT, Shapiro AMJ. Changes in liver enzymes after clinical islet transplantation. Transplantation 76:1280-1284, Nov 2003.

164. Suleman S, Johnson E, Lakey JRT, Merani S, Moore RB, Young ATL. Telomere length analysis as a novel diagnostic test for bladder cancer. Enquires Journal of Interdisciplinary Studies for High School Students 1:1-5, 2003.

165. Murdoch TB, McGhee-Wilson D, Shapiro AMJ, Lakey JRT. Methods of human islet culture for transplantation. Cell Transplantation. 13:605-617, Apr 2004.

166. Tsujimura T, Salehi P, Walker J, Avila J, Madsen K, Lakey JRT, Kuroda Y, Churchill TA. Ameliorating small bowel injury using a cavitary two-layer preservation method with perflurocarbon and nutrient-rich solution. American Journal of Transplantation 4:1421-1428, Apr 2004.

167. Barnett MJ, McGhee-Wilson D, Shapiro AMJ, Lakey JRT. Variation in human islet viability based on different membrane integrity stains. Cell Transplantation 13:481-488, 2004.

168. Woods EJ, Walsh CM, Sidner RA, Zieger MA, Mullin S, Lakey JRT, Ricordi C, Critser JK. Enhanced recovery of cryopreserved islets using SIS. Transplantation Proc. 36:1139-1142, May 2004.

169. Woods EJ, Walsh CM, Sidner RA, Zieger MA, Lakey JRT, Ricordi C, Critser JK. Improved in vitro function of islets using small intestinal submucosa. Transplantation Proc. 36:1175-1177, May 2004.

170. Bhargava R, Senior PA, Ackerman TE, Ryan ED, Paty BW, Lakey JRT, Shapiro AMJ. Prevalence of hepatic steatosis after islet transplantation and its relationship to graft function. Diabetes 53:1311-1317, May 2004.

171. Arata A, Kobayashi N, Okitsu T, Ikeda H, Kobayashi K, Kosaka Y, Narushima M, Matsuoka J, Yamamoto I, Tanaka N, Lakey JRT. Maintenance of glucose-sensitive insulin secretion of cryopreserved human islets with UW solution and ascorbic acid 2 glucoside. American Society for Artificial Internal Organs 28:529-536, 2004.

172. Kobayashi N, Okitsu T, Lakey JRT, Tanaka N. The current situation in human pancreatic islet transplantation: problems and prospects. J Artif. Organs 7:1-8, 2004.

173. Young, ATL, Moore RB, Murray AG, Mullen JC, Lakey JRT. Assessment of different transfection parameters in efficiency optimization. Cell Transplantation 13:179-185, 2004.

174. Rose NL, Palcic MM, Shapiro AMJ, Lakey JRT. Endogenous pancreatic enzyme activity levels show no significant effect on human islet isolation yield. Cell Transplantation 13:153-160, 2004.

175. Salehi P, Madsen K, Zhu J, Castillo E, Avila J, Lakey JRT, Churchill TA. Alleviating ischemia-reperfusion injury in small bowel. American J. of Transplantation 4:728-737, 2004.

176. Street CN, Lakey JRT, Seeberger K, Helms L, Rajotte RV, Shapiro AMJ, Korbutt GS. Heterogenous expression of nestin in human pancreatic tissue precludes its use as an islet precursor marker. Journal of Endocrinology 180:213-225, 2004.

177. Avila JG, Murdoch TB, Troppmann B, MGhee-Wlison D, Hull B, Lakey JRT. Automated cell isolation laboratory information system. Cell Preservation Technoloy 2:209-214, 2004.

178. Street CN, Lakey JRT, Rajotte RV, Shapiro AMJ, Kieffer TJ, Lyon JG, Kin T, Korbutt GS. Enriched human pancreatic ductal cultures obtained from selective death of acinar cells express pancreatic and duodenal homeobox gene-1 in an age-dependently. The Review of Diabetic Studies 1:66-79, 2004.

179. Barnett MJ, Cembrowski G, Shapiro AMJ, Lakey JRT. Saline contamination of University of Wisconsin: Solution preserved human pancreata. Cell Preservation Technology, 2(4):290-295, Jul 2004.

180. Tsujimura T, Kuroda Y, Avila JG, Kin T, Oberholzer J, Shapiro AMJ, Lakey JRT. Influence of pancreas preservation on human islet isolation outcomes: Impact of the two-layer method. Transplantation 78:96-100, Jul 2004.

181. McDonald CG, Ryan EA, Paty BW, Senior PA, Marshall SM, Lakey JRT, Shapiro AMJ. Cross-sectional and prospective association between proinsulin secretion and graft function after clinical islet transplantation. Transplantation 78:934-937, Sep 2004.

182. Street C, Lakey JRT, Shapiro J, Imes S, Rajotte R, Ryan E, Lyon J, Kin T, Avila J, Tsujimura T, Korbutt G. Islet Graft Assessment in the Edmonton Protocol, Implications for predicting long-term clinical outcome. Diabetes 53:3107-3114, Dec 2004.

183. Tsujimura T, Kuroda Y, Churchill TA, Avila JG, Kin T, Shapiro AMJ, Lakey JRT. Short-term storage of the ischemically damaged human pancreas by the two-layer method prior to islet isolation. Cell Transplantation 13:67-73, 2004.

184. Shapiro AMJ, Gallant HL, Hao EG, Lakey JRT, McCready T, Rajotte RV, Yatscoff RW, Kneteman NM. The portal immunosuppressive storm: relevance to islet transplantation? Ther Drug Monit. 27(1):35-37, Feb 2005.

185. Ryan EA, Paty BW, Senior PA, Lakey JRT, Bigam D, Shapiro AM. Beta-score: an assessment of beta-cell function after islet transplantation. Diabetes Care. 28(2):343-347 Feb 2005.

186. Meyer SR, Campbell PM, Rutledge JM, Halpin AM, Hawkins LE, Lakey JRT, Rebeyka IM, Ross DB. Use of an allograft patch in repair of hypoplastic left heart syndrome may complicate future transplantation. European Journal Cardiothorac Surgery. 27(4):554-60, Apr 2005.

187. Kin T, Shapiro AMJ, Lakey JRT. Pancreas divisium: a study of the cadaveric donor pancreas for islet isolation. Pancreas. 30(4):325-327, May 2005.

188. Kin T, Shapiro AMJ, Ryan EA, Lakey JRT. Islet isolation and transplantation from an annular pancreas: a case report. Journal of the Pancreas. 10;6(3):274-276, May 2005.

189. Shapiro AMJ, Lakey JRT, Paty BW, Senior PA, Bigam DL, Ryan EA. Strategic Opportunities in Clinical Islet Transplantation. Transplantation, 79(10):1304-1307, May 2005.

190. Suarez-Pinzon WL, Lakey JRT, Brand SJ, Rabinovitch A. Combination Therapy with Epidermal Growth Factor and Gastrin Induces Neogenesis of Human Islet {beta}-Cells from Pancreatic Duct Cells and an Increase in Functional {beta}-Cell Mass* Journal Clinical Endocrinology Metabolism. 90(6):3401-3409, Jun 2005.

191. Ryan E, Paty B, Senior P, Bigam D, Alfadhli E, Kneteman N, Lakey JRT, Shapiro A. Five-year follow-up after clinical islet transplantation Diabetes 54(7):2060-9, Jul 2005.

192. Meyer S, Nagendran J, Desai L, Rayat G, Churchill T, Anderson C, Rajotte R, Lakey JRT, Ross D. Decellularization reduces the immune response to aortic valve allografts in the rat. The Journal of Thoracic and Cardiovascular Surgery, 2005 130(2:469-76, Aug 2005.

193. Emamaullee JA, Liston P, Korneluk RG, Rajotte RV, Lakey JRT, Shapiro AMJ, and Elliott JF. XIAP Overexpression in Human Islets Prevents Early Post-Transplant Apoptosis and Reduces the Mass Required to Treat Diabetes. Diabetes 54:2541-2548, Sep 2005.

194. Nagendran J, Meyer SR, Webb DNH, Sykes BD, Lakey JRT, Ross DB. H NMR assessment of safe Triton X-100 levels in decellularized rat aortic valve tissue. ,Cell Preservation Technology 3(3):148-154, Sep 2005.

195. Barnett M, Zhai W, LeGatt D, Bang Cheng S, Shapiro AMJ, Lakey JRT. Quantitative Assessment of Collagenase Blends for Human Islet Isolation, Journal of Transplantation 80(6):723-728, Sep 2005.

196. O'Gorman D, Kin T, Murdoch T, Richer B, McGhee-Wilson D, Ryan E, Shapiro AMJ, Lakey JRT. The standardization of pancreatic donors for islet isolations. Transplantation 80(6):801-806, Sep 2005.

197. Narushima M, Kobayashi N, Okitsu T, Tanaka Y, Li S-A, Chen Y, Miki A, Tanaka K, Nakaji S, Takei K, Gutierrez A G, Rivas-Carrillo J D, Navarro-Alvarez N, Jun H-S, Westerman K, Noguchi H, Lakey JRT, Leboulch P, Tanaka N, Yoon J-W, A human B-cell line for transplantation therapy to control type 1 diabetes. Nature Biotechnology 23(10):1274-1282, Oct 2005.

198. Gustavson S, Rajotte R, Hunkeler D, Lakey JRT, Edgerton D, Neal D, Snead W, Penaloza A, Cherrington A. Islet Auto-Transplantation into an Omental or Splenic Site Results in a Normal Beta Cell but Abnormal Alpha Cell Repsonse to Mild Non-Insulin-Induced Hypoglycemia. American Journal of Transplantation 5:2368-2377, Oct 2005.

199. O’Gorman D, McGhee-Wilson D, Shapiro J, Lakey JRT. Multi-lot analysis of custom collagenase in human islet isolations. Transplantation Proceedings 37:3417-3419, Oct 2005.

200. Molinari M, Al-Saif F, Ryan E, Lakey JRT, Senior P, Paty B, Bigam D, Kneteman N, Shapiro J. Sirolimus-induced ulceration of the small bowel in islet transplant recipients: report of two cases, American Journal of Transplantation 5:(11) 2799-2804, Nov 2005.

201. Villiger P, Ryan E, Owen R, O’Kelly K, Oberholzer J, Al Saif F, Kin T, Wang H, Larsen I, Blitz S, Menon B, Senior P, BigamD, Paty B, Kneteman N, Lakey JRT and Shapiro AMJ. Prevention of bleeding after islet transplantation – Lessons learned from a multivariate analysis of 132 cases at a single institution. American Journal of Transplantation 5(12):2992-2998, Dec 2005.

202. Avila J, Barbaro B, Gangemi A, Romagnoli T, Kuechle J, Hansen M, Shapiro J, Testa G, Sankary H, Benedetti E, Lakey JRT, Oberholzer J. Intra-ductal glutamine administration reduces oxidative injury during human pancreatic islet isolation. American Journal of Transplantation 5(12):2830-2837, Dec 2005.

203. Mirbolooki M, Lakey JRT. Oxygen and pancreas preservation. Transplantation 80(12):1, Dec 2005.

204. Seeberger K, Dufour J, Shapiro J, Lakey JRT, Rajotte R, Korbutt G. Expansion of mesenchymal stem cells from human pancreatic ductal epithelium. Laboratory Investigation 86(2):141-53 Feb, 2006.

205. Hao E, Tyrberg B, Itkin-Ansari P, Lakey JRT, Geron I, Monosov E, Barcova M, Mercola M, Levine F. Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas. Nature Medicine 12(3):310-6. Epub Feb 2006.

206. Mirbolooki M, Lakey JRT. Oxygen and pancreas preservation: Reply. Transplantation 81(3): 492, Feb 2006.

207. Lakey JRT, Mirbolooki M, Shapiro AMJ. Current Status of Islet Transplantation. Methods in Molecular Biology. 333: 47-104, Mar 2006.

208. Mirbolooki M, Shapiro AMJ, Lakey JRT. A Perspective on Clinical Islet Transplantation: Past, Present, and Developments for Future. Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents 6(2):191-208, 2006.

209. Senior P, Lakey JRT, Shapiro J, Ryan E, Patty B. Assessment of glycemic control after islet transplantation using the continuous glucose monitor in insulin-independent versus insulin-requiring type 1 diabetes subjects, Diabetes Technology & Therapeutics 8(2):165-173, April 2006.

210. Salehi P, Mirbolooki M, Kin T, Tsujimura T, Shapiro J, Churchill T, Lakey JRT. Ameliorating injury during preservation and isolation of human islets using the two layer method with perfluorocarbon and UW solution. Cell Transplantation 15(2):187-194, 2006.


211. Kin T, Murdoch T, Shapiro AMJ, Lakey JRT. Estimation of pancreas weight from donor variables, Cell Transplantation 15(2):181-185, Apr 2006.

212. Alsaif F, Molinari M, Al-Masloom A, Lakey JRT, Kin T, Shapiro AMJ. Pancreatic islet autotransplantation with completion pancreatectomy in the management of uncontrolled pancreatic fistula after whipple resection for ampullary adenocarcinoma. Pancreas (Letters to the Editor) 32 (4):430-432, May 2006.

213. Miszta-Lane H, Mirbolooki M, Shapiro AMJ, Lakey JRT, Stem Cell Sources for Clinical Islet Transplantation in Type 1 Diabetes: Embryonic or Adult Stem Cells. Medical Hypotheses 67(4):909-913. Epub, Jun 2006.

214. Marzorati S, Antonioli B, Nano R, Maffi P, Piemonti L, Giliola C, Secchi A, Lakey JRT, Bertuzzi F. Culture medium modulates proinflammatory conditions of human pancreatic islets before transplantation. Americal Journal of Transplantation 6:2791-2795, Jul 2006.

215. Fukazawa, T, Matsuoka, J, Naomoto, Y, Nakai, T, Durbin, ML, Kojiama, I, Lakey, JRT, Tanaka, N. Development of a novel beta-cell specific promoter system for the identification of insulin-producing cells in in vitro cell cultures. Exp. Cell Res. 312(17):3404-3412, 2006.

216. Shapiro, AM, Ricordi, C, Hering, BJ, Auchincloss, H, Lindblad, R, Robertson, P, Secchi, A, Brendal, MD, Berney, T, Brennan, DC, Cagliero, E, Alejandro, R, Ryan, E, DiMercurio, B, MNorel, P, Polonsky, KS, Reems, JA, Bretzel, RG, Bertuzzi, F, Froud, T, Kandaswamy, R, Sutherland, DE, Eisenbarth, G, Segal, M, Preiksaitis, J, Korbutt, G, Barton, FB, Viviano, L, Seyfert-Margolis, V, Bluestone, J, Lakey, JRT. International Trial of the Edmonton Protocol for islet transplantation. New England J Medicine. 355(13) 1318-1330, Sep 2006.

217. Bussiere CT, Lakey, JRT, Shaprio, AM, Korbutt, GS. The impact of the mTOR inhibitor sirolimus on the proliferation and function of pancreatic islets and ductal cells. Diabetelologia. 49 (10):2341-2349, Oct 2006.

218. Avila, J, Wang, Y, Barbaro, B, Gangemi, Am Qi, M, Kuechhle, J, Doubleday, M, Churchill, T, Salehi, P, Shapiro, J, Philipson, L, Benedetti, E, Lakey, JRT, Oberholzer, J. Improved outcomes in islet isolation and transplantation by the use of a novel hemoglobin-based O2 carrier. Am J Transplant. 6(12): 2861-2870, 2006.

219. Kin T, Mirbolooki M, Salehi P, Tsukada M, O’Gorman D, Imes S, Ryan EA, Shapiro AM, Lakey JRT. Islet isolation and transplantation outcomes of pancreas preserved with University of Wisconsin solution versus two-layer method using pre-oxygenated perfluorocarbon. Transplantation 82 (10): 1286-90, Nov 2006.

220. Meyer S, Chiu B, Churchill T, Zhu L, Lakey JRT, Ross D. Comparison of Aortic Valve Allograft Decellularization Techniques in the Rat, Journal of Biomedical lMaterials Research: Part A 79(2) 254-262, Nov 2006.

221. Miki, A, Narushima, M, Okitsu, T, Takeno, Y, Soto-Gutierrez, A, Rivas-Carrillo, JD, Navarro-Alvarez, N, Chen, Y, Tanaka, K, Noguchi, H, Masumoto, S, Kohara, M, Lakey, JRT, Kobayashi, E, Tanaka, KN, Koyayashi. Maintenance of mouse, rat and pig pancreatic islet functions by coculture with human islet-derived fibroblasts. . Cell Transplant. 15(4):325-334, 2006.

222. Jiang, H, Secretan, C, Gao, T, Bagnall, K, Korbutt, G, Lakey, JRT, Johma, NM.The development of osteoblasts from stem cells to supplement the fusion of the spine during surgery for AIS. Stud. Health Technol Forum 123:467-472, 2006.

223. Swift S, Kin T, Mirbolooki M, Wilson R, Lakey JRT. Comparison of cooling systems during islet purification. Cell Transplantation. 15(2):175-80, 2006.

224. Merani S, Schur, C, Troung, W, Knutzen, VK, Lakey, JRT, Anderson, CC, Ricordi, C, Shapiro, AM. Compaction of islets is detrimental to transplant outcomes in mice. Transplantation. 82(11):1472-1476, Dec 2006.

225. Kin T, Rosichuk S, Shapiro AMJ, Lakey JRT. Detection of microbial contamination during human islet isolation. Cell Transplantation 16(1):9-13, 2007.

226. Miszta-Lane H, Gill P, Mirbolooki M, Lakey JRT. Effect of Slow-Freezing versus Vitrification on the Recovery of Mouse Embryonic Stem Cells. Cell Preservation Technology. 5(1): 16-24, 2007.

227. Kin T, Zhai X, Murdoch TB, Salam A, Shapiro AMJ, Lakey JRT. Enhancing the success of human islet isolation through optimization and characterization of pancreas dissociation enzyme. American Journal of Transplantation 7:1-8, 2007.

228. Lakey JRT, Mirbolooki M, Rogers C, Mohr J. Supply of human allograft tissue in Canada. Cell and Tissue Banking. 8(2):135-50, 2007.

229. Lakey JRT, Mirbolooki M, Rogers C, Mohr J. Demand for human allograft tissue in Canada. Cell and Tissue Banking. 8 (1): 31-42, 2007.

230. Kin T, Johnson PRV, Shapiro AMJ, Lakey JRT. Factors influencing the collagenase digestion phase of human islet isolation. Transplantation. 82(1):7-12, 2007.

231. Campbell PM, Salam A, Ryan EA, Senior P, Paty BW, Bigam D, McCready T, Halpin A, Imes S, Al Saif F, Lakey JRT, Shapiro AMJ. Pretransplant HLA Antibodies Are Associated with Reduced Graft Survival After Clinical Islet Transplantation. Am J Transplant;7:1242-1248, 2007.

232. Lakey JRT, Kin T, Warnock GL, Shapiro AMJ, Tsapongas P, Imes S, Korbutt GS, Kneteman NM, Rajotte RV, Ryan EA. Long-Term Graft Function After Allogeneic Islet Transplantation. Cell Transplant;16(4):441-446, 2007.

233. Bikopoulos G,daSilva, PA, Lee, SC, Lakey, JRT, Der, SD, Chan, CB, Ceddia, RB, Wheeler, M, Rozakis-Adcock M. Ex vivo transcriptional profiling of human pancreatic islets following chronic exposure to monosaturated fatty acids. J Endocrinology 196(3):455-464, Mar 2008.

234. Suarez-Pinzon, Wilma, L, Lakey, JRT, Rabinovitch, Alex. Combination therapy with glucagon-like peptide-1 and gastrin induces ?-cell neogenesis from pancreatic duct cells in human islets transplanted in immunodeficient diabetic mice. Cell Transplantation. 17: 631-640. 2008.

235. Kin, T, O’Neil, J, Pawlick, R, Korbutt, G, Shapiro, AMJ, Lakey, JRT. The Use of an Approved Biodegradeable Polymer Scaffold as a Solid Support System for Improvement of Islet Engraftment. Artificial Organs, 32(12):990-993. 2008.

236. Mirbolooki MR, Lakey JRT. Pulsatile intravenous insulin therapy: the best practice in reverse diabetes complications? Medical Hypotheses, May 2009. (In Press)

237. Mirbolooki MR, Alexander MA, Foster CE, Imagawa DK, Hoyt DB, Lakey JRT. Rapid pancreas cooling prior to procurement improves the ntracellular energy profile. . Submitted Diabetologia, May 2009.

Book Chapters:

1. Kneteman NM, Lakey JRT, Warnock GL, et al. Pancreas procurement and preservation: impact on islet recovery and viability. In: Ricordi C, ed. Pancreatic Islet Cell Transplantation. Austin, TX: R.G. Landes, pp 72-81, 1992.

2. Rajotte RV, Lakey JRT, Warnock GL. Islet cryopreservation. In: Lanza RP, Chick
WL, eds. Pancreatic Islet Transplantation Volume 1: Procurement of Pancreatic
Islets. Austin, TX: R.G. Landes, pp 137-147, 1994.

3. Warnock GL, Ziliang A, Lakey JRT, Rajotte RV. Islet isolation from canine pancreas. In:
Lanza RP, Chick WL, eds. Pancreatic Islet Transplantation Volume 1: Procurement of
Pancreatic Islets. Austin, TX: R.G. Landes, pp 81-95, 1994.

4. Lakey JRT, Rajotte RV, Warnock GL. Quality control issues for the maintenance of
sterility in the human islet isolation laboratory. Section G: Pancreatic Islets. In:
Ricordi C, ed. Methods in Cell Transplantation. Austin, TX: R.G. Landes, pp 411-419,
1995.

5. Lakey JRT, Rajotte RV, Warnock GL, Kneteman NM. Pancreas procurement for islet isolation and pancreas storage prior to islet isolation. Section G: Pancreatic Islets. In: Ricordi C, ed. Methods in Cell Transplantation. Austin, TX: R.G. Landes, pp 421-431, 1995.

6. Rajotte RV, Lakey JRT, Warnock. Adult islet cryopreservation. Section G: Pancreatic Islets. In: Ricordi C, ed. Methods in Cell Transplantation. Austin, TX: R.G. Landes, pp 517-524, 1995.

7. Rajotte RV, Warnock GL, Lakey JRT. Islet cryopreservation protocols. Chick WL, Lanza RP, Eds. Medical Intelligence Unit: Pancreatic Islet Transplantation Series. RG Landes Company, Austin, 1996.

8. Shapiro AMJ, Ryan EA and Lakey JRT. Pancreatic Islet Transplantation in the Treatment of Diabetes Mellitus. Book chapter in ‘Clinical Endocrinology and Metabolism’. Special Issue on Endocrine Surgery. Guest Editor JR Farndon. Baillière’s Best Practice and Research, Editor, Jeff MP Holly. Vol 15(2):241-264, Jun 2001.

9. Shapiro AMJ, Ryan EA, Paty BW, Lakey JRT. Pancreas and islet transplantation. In: John Pickup J ,Williams G, eds. Textbook of Diabetes, 3rd Edition. Blackwell Science, April 2002.

10. Oberholzer J, Shapiro AMJ, Lakey JRT, Ryan EA, Rajotte RV, Korbutt GS, Morel P. Current status of islet cell transplantation. In: Cameron JL, Evers BM, et al. Advances in Surgery, Vol 37, Mosby, pp 253-282, 2003.

11. Norrie B, Jalil A, Lakey JRT, Rajotte RV, Uludag H. In-situ crosslinking thermoreversible polymers for cell entrapment. In: Hasicri N, Hasicri V, eds. Biomaterials From Molecules to Engineered Tissues, Kluwer Academic/Plenum Publishers, pp 19-28, 2004.

12. Ryan EA, Lakey JRT, Paty BW, Shapiro AMJ. Islet transplantation. In DeFronzo RA, Ferrannini E, Keen h, Zimmet P. International Textbook of Diabetes Mellitus, 3rd Edition. John Wiley & Sons, Ltd, pp 1067-1078, 2004.

13. Paty BW, Ryan EA, Lakey JRT, Shapiro AMJ. Islet Transplantation. In: LeRoith D, Taylor SI, Olefsky JM, eds. Diabetes Mellitus: A Fundamental and Clinical Text, Third Edition. Lippincott Williams & Wilkins, pp 721-732, 2004.

14. Shapiro AMJ, Ryan EA, Lakey JRT. Islet Transplantation in the Treatment of Diabetes Mellitus. In: Best Practice and Research Compendium: Diabetes. Edited by Professor A.H. Barnett. Published by Elsevier Limited, 2005.

15. Shapiro AMJ, Ryan EA, Lakey JRT, Islet transplantation in the treatment of diabetes. Submitted to Rapid Medical Media October, 2005.

16. Lakey JRT, Mirbolooki M, Shapiro J. Current status of Clinical Islet cell transplantation. In: Hornick P, Rose M, eds. Transplantation Immunology, Humana Press, pp 47-103, Mar 2006.

17. Mirbolooki M, Lakey JRT. Pancreas preservation for islet isolation. In: Shapiro AMJ, Shawn AMJ (Eds.) Islet Transplantation and Beta Cell Replacement Therapy, Informa Healthcare, NY, pp 99-114, 2007.

18. Fisher JS, Mirbolooki MR, Lakey JRT, Robertson RP, Marsh CL. Pancreas and Islet Transplantation. In Feehally J, Floege J, Johnson RJ. Comprehensive Clinical Nephrology, 3rdEdition, Mosby/Elsevier, pp 1131-1144, 2007.

19. Mirbolooki M, Lakey JRT, Kin T, Murdoch T, Shapiro AMJ. Aspects and changes of islet isolation. In: Shapiro AMJ, Shawn AMJ, eds. Islet Transplantation and Beta Cell Replacement Therapy. Informa Healthcare, pp 115-134, 2007.

20. Kin, T, Mirbolooki M, Lakey JRT. Design and implementation of a cell processing facility. In: Halberstadt CR, Eerich DF, eds. Cellular Transplantation: From Laboratory to Clinic. Elsevier Inc., pp 629-634 (Chapter 33), 2007.

21. Mirbolooki M, Lakey JRT. Pancreas preservation for islet isolation. In: Islet Transplantation and Beta Cell Replacement Therapy. Shapiro AMJ, Shawn AMJ, eds. Informa Healthcare, pp 99-114, 2007.

22. Marsh C, Mirbolook M, Lakey JRT. Risk factors affecting islet yield function. In: Kandeel FR, ed. Islets: Biology, Immunology, and Clinical Transplantation. Springer (In press. Available Jun 2009).

23. Fisher JS, Mirbolooki MR, Lakey JRT, Robertson RP, Marsh CL. Pancreas and Islet Transplantation. In Feehally J, Floege J, Johnson RJ. Comprehensive Clinical Nephrology, Mosby/Elsevier (In Press 2009).


Books Published

1. Lakey JRT, Kobayashi N, Shapiro JAM, Ricordi C, Okitsu T. Current Human Islet Isolation Protocol. Publisher: Medical Review Co. Ltd., Osaka, Japan, 2004.
   
Grant Grants Submitted Islet Sheet Medical Investigator: Lakey Title: Islet sheet development Support requested: $45,000. Period of Support: 6/15/2009 – 6/15/2010 Support requested and submitted 6/21/09 Amgen Islet Investigator: Lakey Title: Rat islet cell isolation. Support requested: $21,296. Period of Support: 10/2009 – 12/31/2009 Supported requested and submitted 6/21/09 NIH I124 Challenge Grant Co-Investigator: Lakey Title: PET Imaging Agent for Diabetes Mellitus Support Requested & Submitted 6/3/09 IRBAPP #3362 Co-Investigator: Lakey Title: Total pancreatectomy and infusion of auto islet cells for treatment of chronic pancreatitis. Supported Requested & Submitted 5/21/09 NIH/ UCI Proposal #46917 PI: Lakey Title: Enhancing islet cell survival by inhibiting apoptosis. Support Requested & Submitted 4/23/09 FC/ UCI Proposal #46716 PI: Lakey Title: Evaluating donated kidneys at perfusion to increase transplant utilization. Support requested: $79,958. Period of Support: 11/1/2009 – 5/1/2010 Type of Request: New SBIR subcontract proposal. Submitted 4/1/09 JDRF RFA #135650 (Juvenile Diabetes Foundation) Title: Development of Long Term Survival of Alginate Sheets Beta Cell replacement bioengineering PI: Lakey Supported Requested: $150,000. Submitted: 1/12/09 UCI Proposal # 46091 Cerco Medical PI: Lakey Title: Islet Sheet Studies Period of Support: 1/15/09 – 6/30/09 Submitted 1/15/09 JDRF/ UCI Proposal 46079 PI: Lakey/Mirbolooki Title: Enhancing islet cell survival by inhibiting apoptosis Support Requested: $91,772 Period of Support: 6/1/09 – 5/31/2011 New postdoctoral fellowship/Reza Submitted CIRM RFA 08-07 (CIRM Basic biology awards) Co-Investigator: Lakey Molecular regulation of human beta cell progenition proliferation and differentiation North American Foundation for the Cure of Diabetes Request for donation $50,000 for UCI Islet program. Submitted: 12/29/08 FC/ UCI Proposal # 45801- GRIR grant with functional circulation. Title: “Normothermic preconditioning of the porcine pancreas for islet transplant: a feasibility study” Support requested: $79,647. Period of support: 7/1/09-12/31/09 Type of request: New research subcontract. Submitted 12/1/08 NIH Application # 1 R01 NS065148-01 Co-Investigators: Evans/Lakey Title: Tissue engineered nerve constructs for peripheral nerve regeneration. Support Requested: $1338,750. Requested start date: 7/1/2009 Submitted 10/12/2008 Faculty Grant PI: Lakey/Mirbolooki Title: Pancreatic islet death during hypothermic pancreas preservation. Support Requested: $10,639.55 Submitted: 7/10/08 Approval pending (2008-2837) John Brassil/Lakey/Foster/Mirbolooki “Functional circulation LLC 231,648. Submitted IACUC IACUC AP Protocol # 2008-2823 Lead Researcher: Lakey Title: In Vitro Pancreatic Islet Studies Approval Period: 9/26/08 – 9/25/09 Approved 9/26/08 IACU
   
Professional
Societies

American Society of Transplantation
American Diabetes Association
Canadian Society of Transplantation
International Pancreas and Islet Transplant Association
Tissue Engineering Society (Founding Member)
Cell Transplant Society
   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5626
   
Last updated 07/12/2011