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Osvaldo Marinotti

Associate Researcher, Molecular Biology and Biochemistry
School of Biological Sciences

Associate Researcher, Institute for Immunology


Ph.D., University of Sao Paulo- Brazil, 1986, Biochemistry


M.S., University of Sao Paulo- Brazil, 1983, Biochemistry

Phone: (949) 824-3210
Email: omarinot@uci.edu

University of California, Irvine
2315 McGaugh Hall
Mail Code: 3900
Irvine, CA 92697

picture of Osvaldo  Marinotti

Research
Interests
Mosquito, Malaria, Dengue, Genomics, Taxonomy
   
URL dracula.bio.uci.edu/~pedro/OsvaldoIndexServer.html
   
Academic
Distinctions
Master Student Fellowship CNPq - March 1981 – July 1981.
Master Student Fellowship FAPESP - August 1981 - July 1983.
Ph. D. Student Fellowship- FAPESP - August 1983 - July 1986.
Postdoctoral Fellowship- FAPESP- August 1986 - July 1987.
Postdoctoral Fellowship- CNPq - April 1988 - March 1990.
Researcher Fellowship- CNPq - April 1991 – December 2000.
   
Appointments Postdoctoral Fellow, Department of Biochemistry, University of São Paulo, Brazil. August 1986-March 1988. Cloning of genes expressing the yolk proteins of Musca domestica.

Research Fellow in Tropical Public Health. Harvard School of Public Health. Boston, USA, March 1988 - September 1989. Gene expression in the salivary glands of the mosquito Aedes aegypti.

Visiting Postgraduate Researcher. University of California Irvine, Department of Molecular Biology and Biochemistry. Irvine, USA, October 1989 - September 1990. Studies on the molecular biology of vector mosquitoes, continued from the work initiated at Harvard School of Public Health.

Visiting Professor, University of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, USA. July 2000- December 2000. Gene expression in the fat bodies of the malaria vector Anopheles gambiae.
   
Research
Abstract
My research focuses in mosquitoes’ biology and their involvement in the transmission of human diseases.
Mosquito genomics and gene expression
The sequencing of the genomes of Anopheles gambiae and Aedes aegypti is a major achievement. The sequenced genomes present opportunities to improve vector control by revealing new strategies to control pathogen transmission and new gene targets for insecticides and insect repellents. However, a key question to be answered by vector biologists is how to explore this sequenced information in the pursuit of these goals. The analysis of gene expression patterns and the linking of those genes to their biological functions are basic and fundamental steps toward using sequenced data for controlling disease transmission. Our studies utilize a commercial DNA microarray platform and are focused in identifying genes expressed during the adult life of An. gambiae. A publicly available database containing the detailed analyses of specific Anopheles gambiae genes, categorized by their descriptions, functions and levels of expression has been developed (http://www.angagepuci.bio.uci.edu/).
Genetic modification of mosquitoes
Mosquitoes made resistant to pathogens through genetic engineering are proposed as a basis for developing a strategy to control disease transmission. Transgenic approaches that introduce exogenous antipathogen effector genes into mosquito genomes require cis-acting regulatory DNA to control tissue-, stage-, and sex-specific transgene expression. Our efforts are focused in defining cis-acting promoter elements and transcription factors that will provide precise control of transgene expression in mosquitoes.
Mosquito taxonomy
Species identification of malaria vectors (anopheline mosquitoes), can be problematic because many of them belong to complexes of morphologically similar species. Often these species differ in their ecology, behavior and vectorial importance. The application of DNA-based diagnostics has been proven useful for distinguishing between such species. We sequenced the second internal transcribed spacer (ITS2) of ribosomal DNA from several species of Anopheles captured in the Amazon Basin. The ITS2 sequence database proved to be a useful tool for species identification and, potentially, to solve taxonomic problems.
   
Available Technologies
Publications 1. Marinotti, O. and de Bianchi, A.G. (1983) A larval haemolymph protein in the eggs of Rhynchosciara americana. Insect Biochem. 13, 647-653.

2. de Bianchi, A.G. and Marinotti, O., Espinoza-Fuentes, F.P. and Pereira, S.D. (1983) Purification and characterization of Musca domestica storage protein and its developmental profile. Comp. Biochem. Physiol. 76B, 861-867.

3. de Bianchi, A.G. and Marinotti, O. (1984) Storage protein in Rhynchosciara americana (Diptera, Sciaridae). Insect Biochem. 14, 453-461.

4. de Bianchi, A.G. and Marinotti, O. (1985) A specific protein in the genus Rhynchosciara (Diptera, Sciaridae). Experientia 41, 1330-1332.

5. de Bianchi, A.G., Coutinho, M., Pereira, S.D., Marinotti, O. and Targa, H.J. (1985) Vitellogenin and vitellin of Musca domestica: Quantification and synthesis by fat bodies and ovaries. Insect Biochem. 1, 77-84.

6. de Bianchi. A.G. and Marinotti, O. (1986) Physiological pattern and further characterization of the haemolymph violet carotenoprotein of the fly Rhynchosciara americana. Comp. Biochem. Physiol. 83B, 637-642.

7. Marinotti, O. and de Bianchi, A.G. (1986) Structural properties of Musca domestica storage protein. Insect Biochem. 16, 709-716.

8. Marinotti, O. and de Bianchi, A.G. (1986) Uptake of storage protein by Musca domestica fat bodies. J. Insect Physiol. 32, 819-825.

9. de Bianchi, A.G., Capurro, M.L. and Marinotti, O. (1987) Lipophorin in the larval and adult stages of Musca domestica. Arch. Insect Biochem. Physiol. 6, 39-48.

10. Marinotti, O., Nunes, L.R. and de Bianchi, A.G. (1988) Heterogeneous glycosylation of Musca domestica arylphorin. Biochem. Biophys. Res. Com. 151, 1004-1010.

11. Pereira, S.D., Marinotti, O. and de Bianchi, A.G. (1989) Nonvitellogenic female protein in Musca domestica. Arch. Insect Biochem. Physiol. 11, 245-255.

12. Ribeiro, J.M.C., Marinotti, O. and Gonzales, R. (1990) A salivary vasodilator in the blood sucking bug, Rhodnius prolixus. Br. J. Pharmacol. 101, 932-936.

13. Marinotti, O. and James, A.A. (1990) An alpha-glucosidase in the salivary glands of the vector mosquito, Aedes aegypti. Insect Biochem. 20, 619-623.

14. Marinotti, O., James, A.A. and Ribeiro, J.M.C. (1990) Diet and salivation in female Aedes aegypti mosquitoes. J. Insect Physiol. 36, 545-548.

15. James, A.A., Blackmer, K., Marinotti, O., Gohsman, C.R and Racioppi, J.V. (1991) Isolation and characterization of the gene expressing the major salivary gland protein of the female mosquito Aedes aegypti. Mol. Biochem. Parasitology 44, 245-254.

16. Capurro, M.L., de Bianchi, A.G. and Marinotti, O. (1994) Aedes aegypti lipophorin. Comp. Biochem Physiol. 108, 35-39.

17. Marinotti, O., Brito, M. and Moreira, C.K. (1996) Apyrase and ?-glucosidase in the salivary glands of Aedes albopictus. Comp. Biochem. Physiol. 113B, 675-679.

18. Jarrouge, M.G., Capurro, M.L., de Bianchi, A.G. and Marinotti, O. (1997) Major haemolymph protein of Aedes aegypti larvae. Arch. Insect Biochem. Physiol. 34, 191-201.

19. Capurro, M.L., Marinotti, O., Farah, C.S., James, A.A. and Bianchi, A.G. (1997) The non-vitellogenic female protein of Musca domestica is an adult specific hexamerin. Insect Mol. Biol. 6, 97-104.

20. Bracco, J.E., Dalbon, M., Marinotti, O. and Barata, J.M.S. (1997) Resistência a inseticidas organofosforados e carbamatos em população de Culex quinquefasciatus. Rev. Saúde Pública 31, 182-183.

21. Moreira, C.K., Milazzotto, M.P. and Marinotti, O. (1997) Characterization of genes expressed in the salivary glands of Anopheles darlingi. Rev. NPq FCEE Univ. Mackenzie 1, 121-129.

22. Moreira-Ferro, C.K., James, A.A. and Marinotti, O. (1998) A lysozyme in the salivary glands of the malaria vector Anopheles darlingi. Insect Mol. Biol. 7, 257-264.

23. Bracco, J.E., Barata, J.M.S. and Marinotti, O. (1999) Evaluation of the insecticide resistance and its biochemical mechanisms in a population of Culex quinquefasciatus (Diptera: Culicidae) from São Paulo State, Brazil. Mem. Inst. Oswaldo Cruz. 94, 115-120.

24. Moreira-Ferro, C.K., Marinotti, O. and Bijovsky, O. (1999) Morphological and biochemical analyses of the salivary glands of the malaria vector, Anopheles darlingi. Tissue and Cell 31, 264-273.

25. Malafronte, R. S., Marrelli, M.T. and Marinotti, O. (1999) Analysis of ITS2 DNA sequences from Brazilian Anopheles darlingi Root (Diptera: Culicidae) mosquitoes. J. Med. Entomol. 36, 631-634.

26. Marrelli, M.T., Honório, N.A., Flores-Mendoza, C., Lourenço de Oliveira, R., Marinotti, O. and Klotzel, J.K. (1999) Comparative susceptibility of two members of the Anopheles oswaldoi complex, An. oswaldoi and An. konderi, to infection by Plasmodium vivax. Trans. R. Soc. Trop. Med. Hyg. 93, 381-384.

27. Marrelli, M.T., Malafronte, R.S., Flores-Mendoza, C., Lourenço de Oliveira, R., Klotzel, J. K. and Marinotti, O. (1999) Sequence analysis of the Internal Transcribed Spacer of ribosomal DNA in Anopheles oswaldoi (Diptera: Culicidae). J. Med. Entomol. 36, 679-684.

28. Nascimento, E. P., Malafronte, R.S. and Marinotti, O. (2000) The salivary gland proteins of Culex quinquefaciatus mosquitoes. Arch. Insect Biochem. Physiol. 43, 9-15.

29. Capurro, M.L., Moreira-Ferro, C.K., Marinotti, O., James, A.A. and de Bianchi, A.G. (2000) Expression patterns of the larval and adult hexamerin genes of Musca domestica. Insect Mol. Biol. 9, 169-177.

30. Moreira, C.K., Marrelli, M. T., Lima, S.P. and Marinotti, O. (2001) Analysis of the salivary gland proteins of the mosquito, Anopheles darlingi (Diptera: Culicidae) J. Med. Entomol. 38, 763-767.

31. Valenzuela, J.G., Charlab, R., Calvo, E., Miranda-Santos, I.K.F.de, Marinotti,O., Franscischetti, I., and Ribeiro, J.M.C. (2002) The D7 family of salivary proteins in blood sucking diptera. Insect Mol. Biol. 11, 149-155.



32. Labruna, M. Marrelli, M.T., Heinemann, M.B., Fava, A.B., Cortez, A., Soares, R.M., Sakamoto, S.M., Richtzenhain, L.J., Marinotti, O., Schumaker, T.T.S. (2002) Taxonomic status of Ixodes didelphis (Acari:Ixodidae). J. Med. Entomol. 39, 135-142.

33. Calvo, E., de Bianchi, A.G., James, A.A. and Marinotti, O. (2002) The major acid soluble proteins of adult female Anopheles darlingi salivary glands include a member of the D7-related family of proteins. Insect Biochem. Mol. Biol. 32, 1419-1427.

34. Malafronte, R.S., Calvo. E. and Marinotti, O. (2003) The major antigens of Culex quinquefasciatus saliva are D7-related proteins. Insect Biochem. Mol. Biol. 33, 63-71.

35. Moreira, C.K., Capurro, M.L., Calvo, E., Silva Jr., James, A.A, de Bianchi, A.G. and Marinotti, O. (2003) The Musca domestica larval hexamerin is composed of multiple, similar polypeptides. Insect Biochem. Mol. Biol. 33, 389-395.

36. Han, Q., Calvo, E., Marinotti, O., Fang, J., Rizzi, M., James, A.A. and Li, J. (2003) Analysis of the wild-type and mutant genes encoding the enzyme kynurenine monooxygenase of the yellow fever mosquito, Aedes aegypti. Insect Mol. Biol. 12, 483-490.

37. Sais, T.C., Morales, R.M., Ribolla, P.E., de Bianchi, A.G., Marinotti, O. and Bijovsky, A.T. (2003) Morphological aspects of Culex quinquefasciatus salivary glands. Arthropod Structure and Develop. 32, 219-226.

38. Caroci, A.S., Calvo, E., Ribolla, P.E., de Bianchi, A.G. and Marinotti, O. (2003) Two digestive trypsins in three species of neotropical anophelines. J. Med. Entomol. 40, 991-995.

39. Calvo, E., Andersen, J., Francischetti, I.M., Capurro, M. de L., de Bianchi A.G., de James, A.A., Ribeiro, J.M.C. and Marinotti, O. (2004) The transcriptome of adult female Anopheles darlingi salivary glands. Insect Mol. Biol. 13, 73-88.

40. Moreira, C.K., Capurro, M.L., Walter, M., Biessmann, H., James, A.A., de Bianchi, A.G. and Marinotti, O. (2004) Primary characterization and basal promoter activity of two hexamerin genes of Musca domestica. Journal of Insect Science 4:2, Available online:insectscience.org/4.2.

41. Munoz, D., Jimenez A., Marinotti, O. and James A.A. (2004) The AeAct-4 gene is expressed in the developing flight muscles of female Aedes aegypti. Insect Mol. Biol. 13, 563-568.


42. Marrelli, M.T., Floeter-Winter, L.M., Malafronte, R.S., Tadei, W.P., Lourenco-de-Oliveira, R., Flores-Mendoza, C. and Marinotti, O. (2005) Analysis of the second internal transcribed spacers (ITS2) of ribosomal DNA sequences of the anopheline species from Amazon region, South America. Med. Vet. Entomol. 19, 208-217.

43. Xavier N., Marinotti, O. and James A.A. (2005) The accumulation of specific mRNAs following multiple blood meals in Anopheles gambiae. Insect Mol. Biol. 14, 95-103.

44. Marinotti, O., Nguyen, Q.K., Calvo, E.C., James, A.A. and Ribeiro, J.M.C. (2005) Microarray analysis of genes showing variable expression following a bloodmeal in Anopheles gambiae. Insect Mol. Biol. 14, 365-373.

45. Okuda, K. Caroci, A.S., Ribolla, P.E., Marinotti, O., de Bianchi, A.G. and Bijovsky A.T. (2005) Morphological and enzymatic analysis of the midgut of Anopheles darlingi during blood digestion. J. Insect Physiol. 51, 769-776.


46. Arcà B, Lombardo F., Valenzuela J.G., Francischetti I.M.B., Marinotti, O., Coluzzi, M. and Ribeiro, J.M.C. (2005) An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae. J. Exp. Biol. 208, 3971-3986.

47. Li, B., Calvo, E., Marinotti, O., James, A.A. and Paskewitz, S.M. (2005) Characterization of the c-type lysozyme gene family in Anopheles gambiae. Gene 360, 131-139.

48. Marinotti, O., Calvo, E.C., Nguyen, Q.K., Dissanayake, S., Ribeiro, J.M.C. and James A.A. (2006) Genome-wide analysis of gene expression in adult Anopheles gambiae. Insect Mol. Biol.15, 1-12.

49. Marinotti, O., Capurro, M. de L., Nirmala, X., Calvo, E. and James AA. (2006) Structure and expression of the lipophorin-encoding gene of the malaria vector, Anopheles gambiae. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 144, 101-109.

50. Dissanayake, S., Marinotti, O., Ribeiro, J.M.C. and James A.A. (2006) angaGEDUCI: Anopheles gambiae gene expression database with integrated comparative algorithms for identifying conserved DNA motifs in promoter sequences. BMC Genomics 7, 116.

51. Feitosa, F.M., Calvo, E., Merino, E.F., Durham, A.M., James, A.A., de Bianchi, A.G., Marinotti, O. and Capurro, M.de L. (2006) A transcriptome analysis of the Aedes aegypti vitellogenic fat body. Journal of Insect Science 6:06, available online: insectscience.org/6.06.

52. Xavier, N., Marinotti, O., Sandoval, J.M., Phin, S., Gakhar, S., Jasinskiene, N. and James, A.A. (2006) Functional characterization of the promoter of the vitellogenin gene, AsVg1, of the malaria vector, Anopheles stephensi. Insect Biochem. Mol. Biol. 36, 694-700.

53. Marrelli, M.T., Souza, L.F., Marques, R.C., Labruna, M.B., Matioli, S.R., Tonon, A.P., Ribolla, P.E.M., Marinotti, O. and Schumaker, T.T.S. (2007) Taxonomic and phylogenetic relationships between Neotropical species of ticks from genus Amblyomma (Acari:Ixodidae) inferred from second internal transcribed spacer sequences of rDNA. J. Med. Entomol. 44, 222-228.

54. Malafronte, R.S., Marrelli, M.T., Ramirez, C.C.L., Nassar, M.N. and Marinotti, O. (2007) Intraspecific variation of second internal transcribed spacer of nuclear ribosomal DNA among populations of Anopheles (Kerteszia) cruzii (Diptera:Culicidae). J. Med. Entomol. 44, 538-542.

55. Jasinskiene, N., Coleman, J., Ashikyan, A., Salampessy, M., Marinotti, O., and James, A.A. (2007) Genetic control of malaria parasite transmission: Threshold levels for infection in an avian model system. Am. J. Trop. Med. Hyg. 76, 1072-1078.

56. Chen, X.G., Marinotti, O., Whitman, L., Jasinskiene, N. and James, A.A. (2007) Cis-acting regulatory DNA of the Anopheles gambiae vitellogenin gene, VgT2, directs tissue-, stage- and sex-specific expression of a reporter gene in transgenic Anopheles stephensi. Am. J. Trop. Med. Hyg. 76, 1118-1124.

57. Calvo E, Tokumasu F, Marinotti O, Villeval JL, Ribeiro JM, Francischetti IM. (2007) Aegyptin, a novel mosquito salivary gland protein, specifically binds to collagen and prevents its interaction with platelet glycoprotein VI, integrin alpha2beta1, and von Willebrand factor. J Biol Chem. 282, 26928-26938.

58. Okuda K, de Almeida F, Mortara RA, Krieger H, Marinotti O, Tania Bijovsky A (2007) Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus. J Insect Physiol. 53, 1307-1315.

59. Terenius O, Dantas de Oliveira C, Pinheiro WD, Tadei WP, James AA, Marinotti O (2008) 16S rRNA gene sequences from bacteria associated with adult Anopheles darlingi (Diptera:Culicidae) mosquitoes. J. Med. Entomol. 45, 172-175.

REVIEWS

1. Marrelli, M.T., Sallum, M.A.M. and Marinotti, O. (2006) The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin-American anopheline taxonomy – A critical review. Mem. Inst. Oswaldo Cruz 101, 817-832.


PUBLISHED PROCEEDINGS:

1. James, A.A., Blackmer, K., Racioppi, J.V. and Marinotti, O. (1989) Specific gene expression in the salivary glands of vector mosquitoes. In: Host Regulated Developmental Mechanisms in Vector Arthropods. II, 233-237. D. Borovsky and A. Spielman (eds), University of Florida, IFAS, Vero Beach, Florida.
2. Calvo, E., Walter, M., Adelman, Z. N., Jimenez, A., Onal, S., Marinotti, O. and James, A.A. (2005) Nanos (nos) genes of the vector mosquitoes, Anopheles gambiae, Anopheles stephensi and Aedes aegypti. Insect Biochem. Molec. Biol. 35, 789-798.

BOOK CHAPTERS:

1. Marinotti, O. and James, A.A. (2005) The transcriptome of human malaria vectors. In: Malaria: Parasite Biology, Pathogenesis and Protection. Irwin W. Sherwin (Ed) ASM Press, Washington D.C., 516-530.
   
Grant 1) Principal Investigator -FAPESP 90/4476-6 for the study of “Protein Synthesis in Aedes aegypti Salivary Glands”, 1990-1991. 2) Principal Investigator - World Health Organization /TDR Re-Entry Grant 900776 for the study of “Aedes aegypti Salivary Gland Proteins”, 1990-1991. 3) Principal Investigator -CNPq 400298/91-0 for the study of “Aedes aegypti hexamerins”, 1991-1992. 4) Principal Investigator -FAPESP 92/4864-1 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1992-1993. 5) Principal Investigator -FAPESP 93/2941-1 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1993-1994. 6) Principal Investigator -FAPESP 94/0934-0 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1994-1995. 7) Principal Investigator –World Health Organization/TDR 940343 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1994-1995. 8) Principal Investigator -FAPESP 95/2602-8 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1995-1996. 9) Principal Investigator -FAPESP 96/6869-0 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1996-1997. 10) Principal Investigator -FAPESP 97/6158-0 for the study of “Molecular aspects of the Biology of Vector Mosquitoes”, 1997-1998. 11) Principal Investigator -FAPESP 98/14908-2 for the study of “Molecular Aspects of the Biology of Vector Mosquitoes”, 1998-1999. 12) Principal Investigator - (Joint grant Drs. A.G. de Bianchi, S. Daffre and O. Marinotti) FAPESP 98/11372-4 for the study of “Genes, Peptides and Proteins in Arthropods of Medical and Veterinary Importance”, March 1999-March 2004. 13) Principal Investigator- World Health Organization /TDR A20314 for the study of “Microarray analysis of Gene Expression in the Fat Body of Anopheles gambiae Mosquitoes”, 2002-2005.
   
Other Experience Assistant Professor
University of Sao Paulo - Brazil 1987—2000

Associate Professor
University of Sao Paulo - Brazil 2000—2001

   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5532
   
Last updated 05/15/2008