Sukumar Pal

Associate Project Scientist, Pathology
School of Medicine

Ph.D., University of Calcutta, 1988, Zoology (Parasitic Immunology)


Administrative management, University of California, Irvine, 2002

Phone: (949) 824-7450, (949) 293-7541 (cell)
Fax: (949) 824-2160
Email: spal@uci.edu

University of California, Irvine
D440 Medical Sciences 1
Room no. D440
Mail Code: 4800
Irvine, CA 92697

picture of Sukumar  Pal

Research
Interests
Vaccine Development for Chlamydial infections
   
Academic
Distinctions
-1989, Fight for Sight Research Fellowship Award

- 1992, Vector Laboratories Young Investigator Award

-1995, Career Development Award, University of California, Irvine

-2005, Who's Who in America
   
Appointments - Johns Hopkins University, Baltimore, Maryland
- University of California, Irvine, California
   
Research
Abstract
Of all sexually transmitted bacterial diseases, Chlamydia trachomatis infections are the most common ones in all nations of the world. Most pregnant women infected with C. trachomatis transmit infections to their neonates at birth. Neonatal chlamydial infections include inclusion conjunctivitis and pneumonia. In the U.S., the rate of neonatal infection is 8.2 per 1,000 live births. These infections are associated with a high incidence of morbidity and economic loss, but if diagnosed in time, can be treated with antibiotics. However, the use of antibiotics creates several potential problems, including compliance with medicine regimens, development of persistent infections, emergence of antibiotic- resistant strains, and drug allergies. In addition, antibiotic treatment has been associated with enhanced susceptibility to reinfection at the population level. Therefore, development of a therapeutic vaccine or treatment for neonatal infections may be an effective way to counteract these problems. Because we currently lack an appropriate animal model, knowledge of C. trachomatis infections during the neonatal period is limited. Recently, our laboratory developed a neonatal mouse model of the C. trachomatis infection using the C. trachomatis mouse pneumonitis biovar (MoPn). In this model, newborn pups born to previously MoPn-infected dams are inoculated intranasally at 48 hours of age. Pups then are euthanized at various days post-inoculation to monitor bacterial burden in their lungs. This study's overall aim is to identify a Th1 immune component in mediating a C. trachomatis infection in neonates. More specifically, the research will investigate the role of Th1 immunity in resolving C. trachomatis infections during the neonatal period. This study's hypothesis is that a Th1- mediated immunity is needed to resolve a C. trachomatis infection in neonatal life. To test the hypothesis, newborn pups will receive MoPn-specific T-cells, or Th1-modulating/mediating
cytokines, and an intranasal infection will be used to evaluate the efficacy of the adoptive immunity. We predict that neonates with added Th1 immunity will clear C. trachomatis infections more effectively than the non-recipient neonates. The study's result will help us to develop a therapeutic neonatal vaccine against C. trachomatis infections.
PUBLIC HEALTH RELEVANCE: Chlamydia trachomatis infections are a major health problem in both developed and underdeveloped countries. The goal of this proposal is to develop a vaccine for the newborn babies who are born from Chlamydia infected mothers. Decreasing the incidence and prevalence of these infections with a vaccine will have a major health impact worldwide.
   
Publications LIST OF PUBLICATIONS



Published articles in Peer Reviewed Journals



1. G. B. Nair, S. Choudhuri, P. Das, S. Pal, and S. C. Pal. 1984. Improved preservation medium for Campylobacter jejuni. J. Clin. Microbiol. 19 : 288-289.



2. P. Das, S. Pal, and S. C. Pal. 1984. Evaluation of the micro enzyme linked immunosorbent assay, indirect hemagglutination, and indirect fluorescence antibody techniques for the serodiagnosis of amoebiasis. J. Diar. Dis. Res. 2 : 238-242.



3. S. Pal, P. Das, A. C. Ghose, and S. C. Pal. 1984. A preliminary report on the excretory-secretory antigen(s) of Entamoeba histolytica. I.R.C.S Med. Sci. 12 : 620-621.



4. M. K. Bhattacharya, S. Pal, P. Das, S. K. Bhattacharya, P. Dutta, P. P. Chaudhuri, A. K. Chaudhuri, and S. C. Pal. 1984. Profile of intestinal parasites in patients with bowl disorder and gastric dysfunction. Indian J. Publ. Hlth. 28 : 168.



5. P. Das, L. Narain, G. P. Dutta, S. Pal, and S. C. Pal. 1985. Improved method of producing amoebic liver abscesses in hamsters for screening systemically active amoebicides. Australian. J. Exptl. Biol. Med. Sci. 63 : 85-89.



6. P. Das, S. Pal, D. Dutta, M. K. Bhattacharya, and S. C. Pal. 1987. Cryptosporidiosis in Bengali children with acute diarrhea. Trans. Roy. Soc. Trop. Med. Hyg. 81 : 241.



7. M. K. Bhattacharya, S. K. Bhattacharya, P. Das, P. Dutta, S. Pal, P. P. Mukherjee, A. K. Chaudhuri, and S. C. Pal. 1987. Studies in gastric dysfunction in intestinal amoebiasis. Indian J. Physiol. Allied Sci. 41 : 36.



8. S. Pal, S. K. Bhattacharya, P. Das, P. Chaudhuri, P. Dutta, S. P. De, D. Sen, M. R. Saha, G. B. Nair, and S. C. Pal. 1989. Occurrence and significance of Cryptosporidium infection in Calcutta. Trans. Roy. Soc. Trop. Med. Hyg. 83 : 520-521.



9. H. R. Taylor, I. W. Maclean, R. C. Brunham, S. Pal, and J. A. Whittum-Hudson. 1990. Chlamydial heat shock proteins and trachoma. Infect. Immun. 58 : 3061-3063.



10. S. Pal, P. Zhang, R. Hunekee, and H. R. Taylor, J. A. Whittum-Hudson. 1990-91. Evidence for a high frequency of Chlamydia-specific lymphocytes in conjunctiva during ocular infection. Regional Immunology. 3 : 171-176.



11. T. J. Fielder, S. Pal, E. M. Peterson, and L. M. de la Maza. 1991. Sequence of the gene encoding the major outer membrane protein of the mouse pneumonitis biovar of Chlamydia trachomatis. Gene. 106 : 137-138.



12. X. Cheng, S. Pal, L. M. de la Maza, and E. M. Peterson. 1992. Characterization of the humoral response induced by a peptide corresponding to the variable domain IV of the major outer membrane protein of Chlamydia trachomatis, serovar E. Infect. Immun. 60 : 3428-3432.



13. S. Pal, H. R. Taylor, R. Hunekee, R. A. Prendergast, and J. A. Whittum-Hudson. 1992. The frequency of antigen specific B cells during experimental ocular Chlamydia trachomatis infection. Infect. Immun: 60 : 5294-5297.



14. S. Pal, T. J. Fielder, E. M. Peterson, and L. M. de la Maza. 1993. Analysis of the immune response in mice following an intrauterine infection with the Chlamydia trachomatis mouse pneumonitis biovar. Infect. Immun. 61 : 772-776.



15. E. M. Peterson, X. Cheng, S. Pal, and L. M. de la Maza. 1993. The effect of antibody isotype and host cell type on the in vitro neutralization of Chlamydia trachomatis. Infect. Immun. 61: 498-503.



16. P. Das, K. Sengupta, S. Pal, D. Das, and S. C. Pal. 1993. Biochemical and Immunological studies on soluble antigens of Entamoeba histolytica. Parasitol. Res. 79 : 365-271.



17. S. Pal, X. Cheng, E. M. Peterson, and L. M. de la Maza. 1993. Mapping of a surface exposed B-Cell epitope to the variable sequent 3 of the major outer membrane protein of Chlamydia trachomatis. J. General Microbiol. 139 : 1565-1570.



18. S. Pal, T. J. Fielder, E. M. Peterson, and L. M. de la Maza. 1994. Protection of fertility in BALB/c mouse salpingitis model by intranasal immunization with viable Chlamydia trachomatis MoPn biovar. Infect. Immun. 62 : 3354-3362.



19. L. M. de la Maza, S. Pal, A. Khamesipour, and E. M. Peterson. 1994. Intravaginal inoculation of Mice with the Chlamydia trachomatis mouse pneumonitis biovar results infertility. Infect. Immun. 62 : 2094-2097.



20. A. Khamesipour, S. Pal, E. M. Peterson, and L. M. de la Maza. 1994. Induction of infertility by the Chlamydia trachomatis mouse pneumonitis biovar in strains of mice that differ in their response to the 60 kDa heat shock protein. J. Reproduction Fertility. 101 : 287-294.



21. M. Campus, T. P. O'Brien, S. Pal, H. R. Taylor, R. A. Prendergast, J. A. Whittum-Hudson. 1995. An extract of the major outer membrane protein of Chlamydia trachomatis as trachoma vaccine candidate. Invest. Ophthal. Vis. Sci. 36 : 1477-1491.



22. S. Pal, K. Sengupta, B. Manna, S. Sarkar, S. Bhattacharya, and P. Das. 1996. Comparative Evaluation of somatic and excretory-secretory antigens of Entamoeba histolytica in serodiagnosis of human amoebiasis by enzyme linked immunosorbent assay. Indian J. of Medical Research. 104 : 152-156.



23. S. Pal, E. M. Peterson, and L. M. de la Maza. 1996. Intranasal Immunization induces long-term protection in mice against a Chlamydia trachomatis genital challenge. Infect Immun. 64 : 5341-5348.



24. S. Pal, I. Theodore, E. M. Peterson, and L. M. de la Maza. 1997. Monoclonal immunoglobulin A antibody to the outer membrane protein of the Chlamydia trachomatis mouse pneumonitis biovar protect mice against a chlamydial genital challenge. Vaccine 15 : 575-582.



25. S. Pal, I. Theodore, E. M. Peterson, and L. M. de la Maza. 1997. Immunization with a subcellular vaccine corresponding to the outer membrane complex of Chlamydia trachomatis induces protection against a genital challenge. Infect. Immun. 65 : 3361-3369.



26. S. Pal., W. Hui, E. M. Peterson, and L. M. de la Maza. 1998. Factors influencing the induction of infertility in a mouse model of Chlamydia trachomatis ascending genital tract infection. J. Med. Microbiol. 47 : 599-605.



27. S. Pal, K. M. Barnhart, A. M. Abai, E. M. Peterson, and L. M. de la Maza. 1999. Vaccination of mice with DNA plasmids coding for the Chlamydia trachomatis major outer membrane protein elicits an immune response but fails to protect against a genital challenge. Vaccine. 17 : 459-465.



28. K. Bachmair, N. Neu, L. M. de la Maza, S. Pal, A. Hessel, and J. M. Penninger. 1999. Chlamydia infections and heart disease linked through antigenic mimicry. Science. 243 : 1339-1343.



29. S. Pal, E. M. Peterson, L. M. de la Maza. 1999. A murine model for the study of Chlamydia trachomatis genital infections during pregnancy. Infect. Immune. 67 : 2607-10.



30. S. Pal, J. Rangel, E. M. Peterson, and L. M. de la Maza. 1999. Immunogenic and protective ability of the two developmental forms of Chlamydiae in a mouse model of infertility. Vaccine. 18 : 752-61.



31. S. Pal, E. M. Peterson, L. M. de la Maza. 2000. Role of Nramp-1 deletion in Chlamydia infection in mice. Infect. Immune. 68 : 4831-33.



32. S. Pal, E. M. Peterson, and L. M. de la Maza. 2001. Susceptibility of mice to vaginal infection with Chlamydia trachomatis mouse pneumonitis is dependent on the age of animal. Infect. immun. 69 : 6240-47.



33. 29. S. Pal, E. I. Theodore, M. Peterson, L. M. de la Maza. 2001. Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein can elicit a protective immune response against a genital challenge. Infect. Immun. 69 : 6240-47.



34. S. Pal, H. L. Davis, E. M. Peterson, L. M. de la Maza. 2002. Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein by use of CpG oligodeoxynucleotides as adjuvant induces a protective immune response against an intranasal challenge. Infect. Immun.70 : 4812-4817.



35. S. Pal, C. J. Luke, A. G. Barbour E. M. Peterson, L. M. de la Maza. 2003. Immunization with the Chlamydia trachomatis major outer membrane protein using the outer surface protein A of Borrelia burgdoferi as an adjuvant, can induce protection against a chlamydial genital challenge. Vaccine 21 : 1455-1465.



36. S. Pal, E.M. Peterson, L.M. de la Maza. 2003. Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice. Immunology . 110: 368-375.



37. S. Pal, E. M. Peterson, and L. M. de la Maza. 2004. A new murine model for the study of Chlamydia trachomatis genitourinary tract infections in males. Infect. Immun. 72: 4210-16.



38. Yen T-Y, S. Pal, and L. M. de la Maza. 2004. Characterization of the disulfide bonds and free cysteine residues of the Chlamydia trachomatis mouse pneumonitis major outer membrane protein. Biochemistry. 44: 6250-56.



39. S. Pal, E. M. Peterson, and L. M. de la Maza. 2005. Vaccination of newborn mice induces a strong protective immune response against respiratory and genital challenges with Chlamydia trachomatis. Vaccine 23: 5351-5358.



40. S. Pal, E. M. Peterson, R. Rappouli, G. Ratti, and L. M. de la Maza. 2005. Immunization with the Chlamydia trachomatis major outer membrane protein, using adjuvants developed for human vaccines, can induce partial protection in a mouse model against a genital challenge. Vaccine (article in press).



41. S. Pal, E. M. Peterson, and L. M. de la Maza. 2005. Vaccination with the Chlamydia trachomatis major outer membrane protein can elicit an immune response as protective as that resulting from inoculation with live bacteria. Infection and Immunity. 73: 8153-8160.



42. S. Pal, A. P. Schmidt, E. M. Peterson, C. L. Wilson, and L. M. de la Maza. 2005. Role of matrix metalloproteinase-7 in the modulation of a Chlamydia trachomatis infection. Immunology (article in press).



43. G, Sun, S Pal, A. K. sarcon, S. Kim, E. M. Peterson, and L. M. de la Maza. 2005. Structural characterization of the Chlamydia trachomatis mouse pneumonitis major outer membrane protein. J. Biological Chemistry (communicated).





Published articles in Proceedings



1. P. P. Chaudhuri, S. Pal, S. C. Pal, and P. Das. 1988. Studies in Giardia lamblia trophozoites antigens using Sephacryl S-300 column chromatography, polyacrylamide gel electrophoresis and enzyme linked immunosorbent assay. In Advances in Giardia Research. University of Calgary Press. Calgary. pp 191-194.



2. J. A. Whittum-Hudson, S. Pal, P. Zhang, and H. R. Taylor. 1990. In vitro functional studies of conjunctival lymphocytes after ocular Chlamydia trachomatis infection. In Advances in Mucosal Immunology, Proceedings of the 5th International Congress of Mucosal Immunology, T. McDonald et al., (eds.). Kluwer Academic Publishers, Lancaster, U. K.: pp 526-529.



3. J. A. Whittum-Hudson, P. Zhang, S. Pal, and H. R. Taylor. 1990. Chlamydia-specific lymphocytes in conjunctiva in a model of trachoma. In Chlamydial Infection. Proceedings of the 7th International Symposium on Human Chlamydial Infection. W. R. Bowie et al., (eds.). Cambridge University Press, N. Y. U.S.A.: pp 287-290.



4. S. Pal, I. Theodore, E. M. Peterson, and L. M. de la Maza. 1994. Characterization of a neutralizing IgA monoclonal antibody directed against the Chlamydia trachomatis mouse pneumonitis biovar. In Proceedings of the 8th International Symposium on Human Chlamydial Infection, J. Orfila et al; (eds.). Società Esculapio, Italy: pp 141-144.



5. L. M. de la Maza, S. Pal, A. Khamesipour, and E. M. Peterson. 1994. Intravaginal inoculation of Mice with the Chlamydia trachomatis mouse pneumonitis biovar results infertility. In Proceedings of the 8th International Symposium on Human Chlamydial Infection, J. Orfila et al; (eds.). Società Esculapio, Italy: pp 537-540.



6. S. Pal, K. M. Barnhart, A. M. Abai, E. M. Peterson, and L. M. de la Maza. 1998. Immunization of mice with expression plasmids containing DNA sequences corresponding to the C. trachomatis MoPn MOMP failed to protect against a genital challenge. In Proceedings of the ninth International Symposium on Human Chlamydial Infection. R.S. Stephens et al (eds.) International Chlamydial Symposium, San Francisco, U.S.A.. pp 438-441. Napa, California, U.S.A., June 21-26.



7. S. Pal, E. M. Peterson, and L. M. de la Maza. 1998. Characterization of the immunogenic and protective ability of two developmental-forms of Chlamydia trachomatis., In Proceedings of the ninth International Symposium on Human Chlamydial Infection. R.S. Stephens et al (eds.) International Chlamydial Symposium, San Francisco, U.S.A.. pp 450-53. Napa, California, U.S.A., June 21-26.
   
Grant Neonetal Immunity against C. trachomatis Infection
   
Professional
Societies
American Society for Microbiology
Chlamydia Basic Research Society
   
Other Experience
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5324
   
Last updated 09/19/2010