Associate Professor, Developmental & Cell Biology
|Golgi apparatus, centrosome, cell cycle regulation, Chlamydia, pathogenesis|
2010 American Cancer Society Research Scholar
2010 Golden Apple Award for Teaching - School of Biological Sciences UCI
2011 Distinguished Assistant Professor Award for Teaching - UCI Senate
The Golgi apparatus of interphase mammalian cells is located in close physical proximity to the centrosome, the major microtubule organizing center of the cell. The reason for this specific localization of the Golgi apparatus, which is unique to mammalian cells, is not well understood.
There is increasing evidence in support of a functional link between the Golgi apparatus and the centrosome during interphase and mitosis. Our research focuses on determining the mechanism by which Golgi proteins regulate the centrosome during the cell cycle. Specifically, we are interested in how the peripheral Golgi proteins, GM130 and GRASP65, control the organization and function of the centrosome during interphase and mitosis, respectively. In our studies, we are using a broad repertoire of techniques of cell and molecular biology and of biochemistry.
This pericentriolar region a mammalian cell is also occupied by the membrane-bound inclusion that is inhabited by the gram-negative bacterium Chlamydia trachomatis. Chlamydiae have to acquire lipids from the host cell in order to grow and replicate. We are interested in understanding the molecular mechanism by which Chlamydia reroutes host trafficking pathways to obtain host host derived proteins and lipids.
We are also investigating how the chlamydial inclusion interacts with the Golgi apparatus, the ER and the centrosome.
Schnermann MJ, Beaudry CM, Genung NE, Canham SM, Untiedt NL, Karanikolas BD, Sütterlin C, Overman LE (2011): "Divergent synthesis and chemical reactivity of bicyclic lactone fragments of complex rearranged spongian diterpenes." J Am Chem Soc. 133:17494-503
A Kodani, Tonthat, V, Wu B and Sütterlin, C. (2010): Par6alpha interacts with the dynactin subunit p150Glued and is a critical regulator of centrosomal protein recruitment (Mol. Biol. Cell 21:3376-85
Schnermann MJ, Beaudry CM, Egorova AV, Polishchuk RS, Sütterlin C*, Overman LE* (2010): Golgi-modifying properties of macfarlandin E and the synthesis and evaluation of its 2,7-dioxabicyclo[3.2.1]octan-3-one core. PNAS 107:6158-63, PMCID: PMC2851978, * joint corresponding authors
A. Kodani and Sütterlin, C (2009): A new function for an old organelle: Microtubule nucleation at the Golgi apparatus (EMBO J., 22: 995-996)
K. Johnson, Tan, M., and Sütterlin, C (2009): Centrosome abnormalities during a Chlamydia trachomatis infection are caused by dysregulation of the normal duplication pathway (Cellular Microbiology,11:1064-1073)
A. Hasegawa, Sogo, LF., Tan, M. and Sütterlin, C (2009): The host complement regulatory protein, CD59, is transported to the chlamydial inclusion by a Golgi-independent pathway (Infection and Immunity, 77:1285-92)
A. Kodani, Kristensen, I, Huang, L and Sütterlin, C (2009): GM130-dependent control of Cdc42 activity at the Golgi regulates centrosome organization (Mol. Biol. Cell 20:1192-200)
A. Kodani and Sütterlin, C (2008): the Golgi protein GM130 regulates centrosome organization and function. Mol. Biol. Cell 19:745-53
Nakagomi S, Barsoum MJ, Bossy-Wetzel E, Sütterlin C, Malhotra V, Lipton SA. (2008) A Golgi fragmentation pathway in neurodegeneration. Neurobiol Dis. 29:221-31
C. Sütterlin, Polishchuk, R., Pecot, M. and Malhotra, V (2005). The Golgi-associated protein GRASP65 regulates spindle dynamics and is essential for cell division. Mol. Biol. Cell 16, 3211- 3222
Colanzi, A., Sütterlin, C. and Malhotra, V. (2003). RAF-1 activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi fragmentation in mitosis. J. Cell Biol. 161:27-32
Colanzi, A., Sütterlin, C. and Malhotra, V. (2003). Cell-cycle specific Golgi fragmentation: how and why? Curr. Op. Cell Biol. 15, 1-6. Equal contribution of the first 2 authors.
Sütterlin, C., Hsu, P., Mallabiabarrena and Malhotra, V. (2002): Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells. Cell 109, 359-369
Sütterlin, C., Lin, C-Y., Feng, Y., Ferris, D., Erikson, R.L. and Malhotra, V.(2001): Polo-like kinase is required for the fragmentation of the pericentriolar Golgi stacks during mitosis. PNAS 98, 9128-9132
Nagamune, K., Nozaki, T., Maeda, Y., Ohishi, K., Schwarz, R.T., Sütterlin, C., Brun, R., Riezman, H. and Kinoshita, T. (2000): “Critical Roles for glycosylphosphatidylinositol for Trypanosoma brucei.” PNAS 97, 10336-10341
Zalonari, B., Friant, S., Funato, K., Sütterlin, C., Stevenson, B.J., and Riezman, H. (2000): Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae.” EMBO J. 19, 2824-2833
Maser, P., Sütterlin, C., Kralli, N. and Kaminsky R. (1999): “ A nucleoside transporter from Trypanosoma brucei involved in drug resistance” Science 285, 242-244
Sütterlin, C., Escribano, M.V., Gerold, P., Maeda, Y., Mazon, M., Kinoshita, T., Schwarz, R.T. and Riezman, H. (1998): “Saccharomyces cerevisiae GPI10, the functional homolog of human PIG-B is required for GPI-anchor synthesis.” Biochem. J. 15, 153-159
Sütterlin, C., Horvath, A., Gerold, P., Schwarz, R.T., Wang, Y., Dreyfuss, M. and Riezman, H. (1997): “Identification of a species-specific inhibitor of glycosylphosphatidylinositol synthesis.” EMBO J.16, 6374-6383
Sütterlin, C., Doering, T.L., Schimmöller, F., Schröder, S. and Riezman, H. (1997): “Specific requirements for ER to Golgi transport of GPI-anchored proteins in yeast.” J. Cell Sci.110, 2703-2714
Horvath, A., Sütterlin, C. , Mannig-Krieg, U., Movva, N.R. and Riezman, H. (1994): “Ceramide synthesis enhances transport of GPI-anchored proteins to the Golgi in yeast.” EMBO J. 13, 3687-3695
Schulz, S.J., Fry, A., Sütterlin, C., Ried, T. and Nigg E.A. (1994) Cell Growth and Differentiation 5, 625-635: “Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans”
|Grants||NIGMS: R01GM089913: "Mechanism of centrosome regulation from the Golgi" 05/10 - 4/15|
|American Cancer Society Research Scholar Grant RSG-10-250-01-CCG: " Does Chlamydia contribute to cancer through centrosome dysregulation?" 7-07/10 - 06/14|
American Society of Cell Biology
American Society of Microbiology
|Link to this profile||http://www.faculty.uci.edu/profile.cfm?faculty_id=5237|