Stephanie Chee-Yee Tjen-A-LooiProject Scientist, Susan Samueli Center for Integrative Medicine |
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Research Interests |
Cardiovascular regulation, Neurophysiology, Acupuncture | |
| URL | www.sscim.uci.edu/ | |
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Academic Distinctions |
Faculty Career Development Award, University of California, Irvine Sigma XI COM most innovative topic poster award, University of California, Irvine Post-doctoral NIH Fellowship Award Proctor and Gamble Graduate Student Award, American Physiology Society Department of Health and Biomedical Science Student Award, UW-Madison |
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Research Abstract |
The most pertinent research areas are cardiovascular regulation and neurophysiology. These studies are interrelated and allow us to understand the neuronal pathways involved in the integration of visceral and somatic afferents in the central nervous system such as the medullary, midbrain and hypothalamic regions. The increase in blood pressure occurring during myocardial ischemia activating cardiac afferents or gastric distention as well as abdominal ischemia innervating the visceral afferent nerve fibers involves autonomic reflex responses. Stimulation of the visceral afferents leads to excitatory reflexes and involves N-methyl-D-aspartate (NMDA), non-NMDA spinal receptors, endothelial (nitric oxide) NO, neuronal NO, free radicals and among others. Interestingly, electroacupuncture (EA) through the stimulation of somatic nerves reduces the visceral sympathoexcitatory reflexes through specific opioid receptors in the rostral ventral lateral medullary (rVLM) neurons. Recent studies have demonstrated that the neurons in the cardiovascular premotor sympathetic rVLM as well as the ventrolateral periaqueductal midbrain and arcuate hypothalamus process the convergence of somatic and visceral input during acupuncture stimulation. Ongoing studies investigate the underlying mechanisms and neuronal pathways involved with modulation of the hypertensive responses. Neurochemicals opioids, endocannabinoid, GABA, glutamate, acetylcholine, nociceptin and serotonin contribute to the action of acupuncture and few have been identified to play a role in the long lasting effect of EA. Genetic changes may also contribute to the prolonged action of acupuncture. | |
| Publications |
Book Chapters: Longhurst, J. C., Tjen-A-Looi, S. , Fu, L-W.: Cardiac sympathetic afferent activation provoked by myocardial ischemia and reperfusion: Mechanisms and reflexes. Eds. M.P. Chapleau, F. Abboud In: Neuro-Cardiovascular Regulation : From Molecule to Man. The New York Academy of Sciences 940:74-95. 2001 Tjen-A-Looi SC and JC Longhurst. Mechanistic and Physiologic Links between CAM and the Cardiovascular System. Mechanisms of Cardiovascular Action of Acupuncture. Eds. MW Krucoff and J.H.K Vogel. In: Integrative Cardiology, chapter VII b. Medical Publishing, McGraw-Hill. 2007 |
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Peer Reviewed Articles: Tjen-A-Looi, S., Lippton, H., Cary, J., Keith, I. M.: CGRP and Somatostatin modulate chronic hypoxic pulmonary hypertension. American Journal of Physiology, 263:H681-H690. 1992 Tjen-A-Looi, S., Ekman, R., Osborn, J., Keith, I.: Pulmonary vascular effects by Endothelin-1 in normoxia and chronic hypoxia: a longitudinal study. American Journal of Physiology, 271:H2246-H2253. 1996 Tjen-A-Looi, S., Bonham, A., Longhurst, J.: Interactions between cardiac sympathetic and cardiac vagal afferents in the nucleus tractus solitarius. American Journal of Physiology, 272:H2843-H2851. 1997 Tjen-A-Looi, S., Kraiczi, H., Ekman, R.,Keith, I. M.: Sensory CGRP depletion by capsaicin exacerbates hypoxia-induced pulmonary hypertension in rats. Regulatory Peptides 74:1-10. 1998 Tjen-A-Looi, S., Pan, H-L., Longhurst, J.: Endogenous bradykinin activates ischemically sensitive cardiac visceral afferents through kinin B2-receptors. Journal of Physiology 510.2:633-641. 1998 Chao, D. M., Shen, L. L., Tjen-A-Looi, S., Pitsillides, K. F., Li, P., Longhurst, J, C.: Naloxone reverses inhibitory effect of electroacupuncture on sympathetic cardiovascular reflex responses. American Journal of Physiology 267:H2127-H2134. 1999 Keith, IM., Tjen-A-Looi, S., Kraiczi, H., Ekman, K.: Three-week neonatal hypoxia reduces blood CGRP and causes persistent pulmonary hypertension rats. American Journal of Physiology 279:H1571-H1578. 2000 Li, P., Tjen-A-Looi, S., Longhurst, J.: Rostral ventrolateral medullary opioid receptor subtypes in the inhibitory effect of electroacupuncture on reflex autonomic response in cats. Autonomic Neuroscience: Basic and Clinical 89:38-47. 2001 Tjen-A-Looi, S., Phan, N., Longhurst, J. C.: Nitric oxide modulates sympathoexcitatory cardiac-cardiovascular reflexes elicited by bradykinin. American Journal of Physiology 281:H2010-H2017. 2001 Gee, BY., Tjen-A-Looi, S., Hill, J., Chadal, P., Longhurst, J.: Role of spinal NMDA and non-NMDA receptors in the pressor reflex response to abdominal ischemia. American Journal of Physiology 282:R850-R857. 2002 Tjen-A-Looi, S. C., Fu, L-F, Longhurst, J. C.: Xanthine oxidase, but not neutrophils, contributes to activation of cardiac sympathetic afferents during myocardial ischemia in cats. Journal of Physiology 543.1:327-336. 2002 Li, P., Rowshan, K., Crisostomo, M., Tjen-A-Looi, S. C., Longhurst, J. C.: Effect of electroacupuncture on pressor reflex during gastric distention in rats. American Journal of Physiology 283:R1335-R1345. 2002 Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Prolonged Inhibition of Rostral Ventral Lateral Medullary Premotor Sympathetic Neurons by Electroacupuncture. Autonomic Neuroscience: Basic and Clinical. 453(2):119-131. 2003 Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Medullary substrate and differential cardiovascular response during stimulation of specific acupoints. American Journal of Physiology 287:R852-R862. 2004 Zhou, W., Fu, L., Tjen-A-Looi, S. C., Li, P., Longhurst, J. C.: Afferent Mechanisms Underlying Stimulation Modality-Related Modulation of Acupuncture-Related Cardiovascular Responses. J Appl Physiol 98(3):872-880. 2005 Tjen-A-Looi, S.C., Fu, LW, Zhou, W. (Yi, S.), Longhurst, J.C.: Role of Unmyelinated Fibers in Electroacupuncture-Cardiovascular Responses. Autonomic Neuroscience: Basic and Clinical. 118:43-50. 2005 Crisostomo, M. M., Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: Nociceptin in rVLM Mediates Electroacupuncture Inhibition of Cardiovascular Reflex Excitatory Response in Rats. Journal of Applied Physiology 98:2056-2063. 2005 Zhou, W., Tjen-A-Looi, S. C., Longhurst, J. C.: Brain Stem Mechanisms Underlying Acupuncture Modality-Related Modulation of Cardiovascular in rats. Journal of Applied Physiology 99(3):851-60. 2005 Zhou, W., Tjen-A-Looi, S. C., Longhurst, J. C.: Brain Stem Mechanisms Underlying Acupuncture Modality-Related Modulation of Cardiovascular in rats. Journal of Applied Physiology 99(3):851-60. 2005 Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: Role of hypothalamus and midbrain in electroacupuncture. American Journal of Physiology, Heart and Circulatory Physiology 209:H2523-H2542. 2006 Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Midbrain vlPAG inhibits rVLM in cardiovascular sympathoexcitatory responses during electroacupuncture. American Journal of Physiology, Heart and Circulatory Physiology 209:H2543-H2553. 2006 Zhou, W., Fu, L-W, Tjen-A-Looi S. C., Guo, Z-L, Longhurst J. C.: Glutamate's Role in a Visceral Sympathoexcitatory Reflex in Rostral Ventrolateral Medulla of Cats. American Journal of Physiology, Heart and Circulatory Physiology 291(3):H1309- H1318. 2006 Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Role of Medullary GABA, Opioids and Nociceptin in Prolonged Inhibition of Cardiovascular Sympathoexcitatory Reflexes during Electroacupuncture in Cats. American Journal of Physiology 293: H3627-H3635. 2007 |
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Guo, Z-L., Moazzami, A., Tjen-A-Looi, S. C., Longhurst, J. C.: Responses of opioid and serotonin containing medullary raphe neurons to electroacupuncture. Brain Research. 1229:125-36. 2008. Li, P., Tjen-A-Looi, S. C., Guo, Zhi-Ling, Fu, Liang-Wu, Longhurst, J. C.: Long-Loop Pathways in Cardiovascular Electroacupuncture Responses. Journal of Applied Physiology 106(2):620-30. 2009. Tjen-A-Looi, S. C., Li, P., Longhurst, J. C.: Processing Cardiovascular Information in the vlPAG during Electroacupuncture in Rats: Roles of Endocannabinoids and GABA. Journal of Applied Physiology 106(6):1793-9. 2009. Guo, Z-L., Tjen-A-Looi, S. C., Fu, L-W., Longhurst, J. C.: Nitric oxide in rostral ventrolateral medulla regulates cardiac-sympathetic responses: role of synthase isoforms. American Journal of Physiology. 297(4):H1478-86. 2009. Moazzami, A., Tjen-A-Looi, S. C.*, Guo, ZL, Longhurst, J. C.; Serotonergic projection from nucleus raphe pallidus to rostral ventrolateral medulla modulates cardiovascular reflex responses during acupuncture. J Applied Physiology 108: 1336-1346. *Share first authorship. 2010. Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: The Arcuate-Ventrolateral Periaqueductal Gray Reciprocal Circuit in Electroacupuncture Cardiovascular Inhibition. Autonomic Neuroscience: Basic and Clinical 158:13-23. 2010. |
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| Grant | Co-PI and co-investigator on NIH grants: HL-63313, HL-72125 | |
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Professional Societies |
American Physiological Society American Association for the Advancement of Science Society for Neuroscience |
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| Other Experience | ||
| Graduate Programs |
Comparative Physiology |
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| Research Center | Susan Samueli Center for Integrative Medicine | |
| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=5153 | |
| Last updated | 01/26/2012 | |