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Stephanie Chee-Yee Tjen-A-Looi

Project Scientist, Susan Samueli Center for Integrative Medicine
School of Medicine

Ph.D., University of Wisconsin, Madison, 1994, Animal Health and Biomedical Sciences/Physiology


M.S., University of Wisconsin, Madison, 1991, Veterinary Science


B.S., University of California, Davis, 1987, Nutrition Science

Phone: (949) 824-8161
Email: stjenalo@uci.edu

University of California, Irvine
C240 Medical Science
Mail Code: 4075
Irvine, CA 92697

picture of Stephanie Chee-Yee Tjen-A-Looi

Research
Interests
Cardiovascular regulation, Neurophysiology, Acupuncture
   
URL www.sscim.uci.edu/
   
Academic
Distinctions
Faculty Career Development Award, University of California, Irvine
Sigma XI COM most innovative topic poster award, University of California, Irvine
Post-doctoral NIH Fellowship Award
Proctor and Gamble Graduate Student Award, American Physiology Society
Department of Health and Biomedical Science Student Award, UW-Madison
   
Research
Abstract
The most pertinent research areas are cardiovascular regulation and neurophysiology. These studies are interrelated and allow us to understand the neuronal pathways involved in the integration of visceral and somatic afferents in the central nervous system such as the medullary, midbrain and hypothalamic regions. The increase in blood pressure occurring during myocardial ischemia activating cardiac afferents or gastric distention as well as abdominal ischemia innervating the visceral afferent nerve fibers involves autonomic reflex responses. Stimulation of the visceral afferents leads to excitatory reflexes and involves N-methyl-D-aspartate (NMDA), non-NMDA spinal receptors, endothelial (nitric oxide) NO, neuronal NO, free radicals and among others. Interestingly, electroacupuncture (EA) through the stimulation of somatic nerves reduces the visceral sympathoexcitatory reflexes through specific opioid receptors in the rostral ventral lateral medullary (rVLM) neurons. Recent studies have demonstrated that the neurons in the cardiovascular premotor sympathetic rVLM as well as the ventrolateral periaqueductal midbrain and arcuate hypothalamus process the convergence of somatic and visceral input during acupuncture stimulation. Ongoing studies investigate the underlying mechanisms and neuronal pathways involved with modulation of the hypertensive responses. Neurochemicals opioids, endocannabinoid, GABA, glutamate, acetylcholine, nociceptin and serotonin contribute to the action of acupuncture and few have been identified to play a role in the long lasting effect of EA. Genetic changes may also contribute to the prolonged action of acupuncture.
   
Publications Book Chapters:
Longhurst, J. C., Tjen-A-Looi, S. , Fu, L-W.: Cardiac sympathetic afferent activation provoked by myocardial ischemia and reperfusion: Mechanisms and reflexes. Eds. M.P. Chapleau, F. Abboud In: Neuro-Cardiovascular Regulation : From Molecule to Man. The New York Academy of Sciences 940:74-95. 2001

Tjen-A-Looi SC and JC Longhurst. Mechanistic and Physiologic Links between CAM and the Cardiovascular System. Mechanisms of Cardiovascular Action of Acupuncture. Eds. MW Krucoff and J.H.K Vogel. In: Integrative Cardiology, chapter VII b. Medical Publishing, McGraw-Hill. 2007
   
  Peer Reviewed Articles:
Tjen-A-Looi, S., Lippton, H., Cary, J., Keith, I. M.: CGRP and Somatostatin modulate chronic hypoxic pulmonary hypertension. American Journal of Physiology, 263:H681-H690. 1992

Tjen-A-Looi, S., Ekman, R., Osborn, J., Keith, I.: Pulmonary vascular effects by Endothelin-1 in normoxia and chronic hypoxia: a longitudinal study. American Journal of Physiology, 271:H2246-H2253. 1996

Tjen-A-Looi, S., Bonham, A., Longhurst, J.: Interactions between cardiac sympathetic and cardiac vagal afferents in the nucleus tractus solitarius. American Journal of Physiology, 272:H2843-H2851. 1997

Tjen-A-Looi, S., Kraiczi, H., Ekman, R.,Keith, I. M.: Sensory CGRP depletion by capsaicin exacerbates hypoxia-induced pulmonary hypertension in rats. Regulatory Peptides 74:1-10. 1998

Tjen-A-Looi, S., Pan, H-L., Longhurst, J.: Endogenous bradykinin activates ischemically sensitive cardiac visceral afferents through kinin B2-receptors. Journal of Physiology 510.2:633-641. 1998

Chao, D. M., Shen, L. L., Tjen-A-Looi, S., Pitsillides, K. F., Li, P., Longhurst, J, C.: Naloxone reverses inhibitory effect of electroacupuncture on sympathetic cardiovascular reflex responses. American Journal of Physiology 267:H2127-H2134. 1999

Keith, IM., Tjen-A-Looi, S., Kraiczi, H., Ekman, K.: Three-week neonatal hypoxia reduces blood CGRP and causes persistent pulmonary hypertension rats. American Journal of Physiology 279:H1571-H1578. 2000

Li, P., Tjen-A-Looi, S., Longhurst, J.: Rostral ventrolateral medullary opioid receptor subtypes in the inhibitory effect of electroacupuncture on reflex autonomic response in cats. Autonomic Neuroscience: Basic and Clinical 89:38-47. 2001

Tjen-A-Looi, S., Phan, N., Longhurst, J. C.: Nitric oxide modulates sympathoexcitatory cardiac-cardiovascular reflexes elicited by bradykinin. American Journal of Physiology 281:H2010-H2017. 2001

Gee, BY., Tjen-A-Looi, S., Hill, J., Chadal, P., Longhurst, J.: Role of spinal NMDA and non-NMDA receptors in the pressor reflex response to abdominal ischemia. American Journal of Physiology 282:R850-R857. 2002

Tjen-A-Looi, S. C., Fu, L-F, Longhurst, J. C.: Xanthine oxidase, but not neutrophils, contributes to activation of cardiac sympathetic afferents during myocardial ischemia in cats. Journal of Physiology 543.1:327-336. 2002

Li, P., Rowshan, K., Crisostomo, M., Tjen-A-Looi, S. C., Longhurst, J. C.: Effect of electroacupuncture on pressor reflex during gastric distention in rats. American Journal of Physiology 283:R1335-R1345. 2002

Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Prolonged Inhibition of Rostral Ventral Lateral Medullary Premotor Sympathetic Neurons by Electroacupuncture. Autonomic Neuroscience: Basic and Clinical. 453(2):119-131. 2003

Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Medullary substrate and differential cardiovascular response during stimulation of specific acupoints. American Journal of Physiology 287:R852-R862. 2004

Zhou, W., Fu, L., Tjen-A-Looi, S. C., Li, P., Longhurst, J. C.: Afferent Mechanisms Underlying Stimulation Modality-Related Modulation of Acupuncture-Related Cardiovascular Responses. J Appl Physiol 98(3):872-880. 2005

Tjen-A-Looi, S.C., Fu, LW, Zhou, W. (Yi, S.), Longhurst, J.C.: Role of Unmyelinated Fibers in Electroacupuncture-Cardiovascular Responses. Autonomic Neuroscience: Basic and Clinical. 118:43-50. 2005

Crisostomo, M. M., Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: Nociceptin in rVLM Mediates Electroacupuncture Inhibition of Cardiovascular Reflex Excitatory Response in Rats. Journal of Applied Physiology 98:2056-2063. 2005

Zhou, W., Tjen-A-Looi, S. C., Longhurst, J. C.: Brain Stem Mechanisms Underlying Acupuncture Modality-Related Modulation of Cardiovascular in rats. Journal of Applied Physiology 99(3):851-60. 2005

Zhou, W., Tjen-A-Looi, S. C., Longhurst, J. C.: Brain Stem Mechanisms Underlying Acupuncture Modality-Related Modulation of Cardiovascular in rats. Journal of Applied Physiology 99(3):851-60. 2005

Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: Role of hypothalamus and midbrain in electroacupuncture. American Journal of Physiology, Heart and Circulatory Physiology 209:H2523-H2542. 2006

Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Midbrain vlPAG inhibits rVLM in cardiovascular sympathoexcitatory responses during electroacupuncture. American Journal of Physiology, Heart and Circulatory Physiology 209:H2543-H2553. 2006

Zhou, W., Fu, L-W, Tjen-A-Looi S. C., Guo, Z-L, Longhurst J. C.: Glutamate's Role in a Visceral Sympathoexcitatory Reflex in Rostral Ventrolateral Medulla of Cats. American Journal of Physiology, Heart and Circulatory Physiology 291(3):H1309- H1318. 2006

Tjen-A-Looi, S. C., Li, P. and Longhurst, J. C.: Role of Medullary GABA, Opioids and Nociceptin in Prolonged Inhibition of Cardiovascular Sympathoexcitatory Reflexes during Electroacupuncture in Cats. American Journal of Physiology 293: H3627-H3635. 2007
   
  Guo, Z-L., Moazzami, A., Tjen-A-Looi, S. C., Longhurst, J. C.: Responses of opioid and serotonin containing medullary raphe neurons to electroacupuncture. Brain Research. 1229:125-36. 2008.

Li, P., Tjen-A-Looi, S. C., Guo, Zhi-Ling, Fu, Liang-Wu, Longhurst, J. C.: Long-Loop Pathways in Cardiovascular Electroacupuncture Responses. Journal of Applied Physiology 106(2):620-30. 2009.

Tjen-A-Looi, S. C., Li, P., Longhurst, J. C.: Processing Cardiovascular Information in the vlPAG during Electroacupuncture in Rats: Roles of Endocannabinoids and GABA. Journal of Applied Physiology 106(6):1793-9. 2009.

Guo, Z-L., Tjen-A-Looi, S. C., Fu, L-W., Longhurst, J. C.: Nitric oxide in rostral ventrolateral medulla regulates cardiac-sympathetic responses: role of synthase isoforms. American Journal of Physiology. 297(4):H1478-86. 2009.

Moazzami, A., Tjen-A-Looi, S. C.*, Guo, ZL, Longhurst, J. C.; Serotonergic projection from nucleus raphe pallidus to rostral ventrolateral medulla modulates cardiovascular reflex responses during acupuncture. J Applied Physiology 108: 1336-1346. *Share first authorship. 2010.

Li, P., Tjen-A-Looi, S. C., Longhurst, J. C.: The Arcuate-Ventrolateral Periaqueductal Gray Reciprocal Circuit in Electroacupuncture Cardiovascular Inhibition. Autonomic Neuroscience: Basic and Clinical 158:13-23. 2010.
   
Grant Co-PI and co-investigator on NIH grants: HL-63313, HL-72125
   
Professional
Societies
American Physiological Society
American Association for the Advancement of Science
Society for Neuroscience
   
Other Experience
Graduate Programs Comparative Physiology

   
Research Center Susan Samueli Center for Integrative Medicine
   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=5153
   
Last updated 01/26/2012