David M. GardinerProfessor, Developmental & Cell Biology UCI ADVANCE Program Equity Advisor |
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Research Interests |
limbs, development, regeneration, stem cells, endocrine disruption, teratology, amphibian, deformed frogs | |
| URL | regeneration.bio.uci.edu | |
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Academic Distinctions | Fellow American Association for the Advancement of Science | |
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Research Abstract |
Salamanders are the only vertebrates that can regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. Most recently we have been involved in the development of genomic resources for identifying the key signaling pathways that control limb regeneration. The tools and knowledge are in place to attack complex systems, and understanding regeneration is likely to lead to new approaches and therapies for replacing or repairing lost, damaged or diseased parts of the body. | |
| Publications | Ferris, D.R., Satoh, A., Mandefro, B., Cummings, G., Gardiner, D.M. and Rugg, E.L. (2010). Ex vivo generation of a functional and regenerative wound epithelium from axolotl (Ambystoma mexicanum) skin. Dev. Growth Diff. 52:715-724. | |
| Satoh, A., Cummings, G.M.C., Bryant, S.V. and Gardiner, D.M. (2010). Regulation of proximal-distal intercalation during limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Growth Diff. 52: 785-798. | ||
| McCusker, C. and Gardiner, D.M. (2011). The axolotl model for regeneration and aging research: A Mini-Review. Gerontology. 57:565-571. | ||
| Sun, L., Chen, M., Yang, H., Wang, T., Liu, B., Shu, C. and David M. Gardiner, D.M. (2011). 454 sequencing analysis of gene expression profiling during Intestinal and body wall regeneration in the sea cucumber. Comp. Biochem. Physiol. Part D Genomics Proteomics 6:195-205. | ||
| Hirata, A., Gardiner, D.M., and Satoh, A. (2010). Dermal fibroblasts contribute to multiple tissues in the accessory limb model. Dev. Growth & Diff. 52:343-50. | ||
| Satoh, A., Cummings, G.M.C., Bryant, S.V., and Gardiner, D.M. (2010). Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 337:444-457. | ||
| Tanner, K., Ferris, DR., Lanzano, L., Mandefro, B., Mantulin, WW., Gardiner, DM/. Rugg, EL., and Gratton, E. (2009). Coherent movement of cell layers during wound healing by image correlation spectroscopy. Biophysical J. 97:2098-2106. | ||
| Holden, P.K., Li, C., Da Costa, V., Sun, C.H., Bryant, S.V., Gardiner, D.M., and Wong, B.J.F. (2009). The effects of laser irradiation of cartilage on chondrocyte gene expression and the collagen matrix. Lasers in Surgery & Medicine 41:487-491. | ||
| Satoh, A., James, M.A. and Gardiner, D.M. (2009). The role of nerve signaling in limb genesis and agenesis during axolotl limb regeneration. J. Bone Joint Surg. (in press). | ||
| Monaghan, J.R., Epp, L., Putta, S., Page, R.B., Walker, J.A., Beachy, C.K., Zhu, W., Pao, G.M., Verma, I.M., Hunter, T., Bryant, S.V., Gardiner, D.M., Harkins, T.T., and Voss, S.R. (2009). Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration. BMC Biology 7:1 | ||
| Smith, J.J., Putta, S., Zhu, W., Pao,. G., Harkins, T., Hunter, T., Verma, I., Bryant, S.V., Gardiner, D.M., and Voss, S.R. (2009). Genic Regions of a Large Salamander Genome Contain Long Introns and Novel Genes. BMC Genomics 10:19. | ||
| Ghosh, S., Roy, S., Seguin, C., Bryant, S.V. and Gardiner, D.M. (2008). Expression of Wnt5a and Wnt5b in developing and regenerating axolotl limbs. Dev. Growth & Diff. 50:289-297 | ||
| Satoh, A., Bryant, S.V. and Gardiner, D.M. (2008). Regulation of dermal fibroblast dedifferentiation and redifferentiation during wound healing and limb regeneration in the Axolotl. Dev. Growth & Diff. 50:743-754. | ||
| Satoh, A., Graham, G.M.C., Bryant, S.V. and Gardiner, D.M. (2008). Neurotrophic regulation of epidermal dedifferentiation during wound healing and limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 319:321-325. | ||
| Muneoka, K., Han, M., and Gardiner, D. (2008). Human Limb Regeneration: Understanding the Challenge. Scientific American 298:56-63. | ||
| Satoh, A., Gardiner, D.M., Bryant, S.V. and Endo, T. (2007). Nerve-induced ectopic limb blastemas in the axolotl are equivalent to amputation-induced blastemas. Dev. Biol. 312:231-244. | ||
| Gardiner, D.M. and Bryant, S.V. (2007). Homeobox-containing genes in limb regeneration. in “HOX Gene Expression” (ed. Spyros Papageorgiou). Landes Bioscience, Austin. 9 pp | ||
| Grun, F., Watanabe, H., Zamanian, Z., Maeda, L. Arima, K., Chubacha, R., Gardiner, D.M., Kanno, J., Iguchi, T., and Blumberg, B. (2006). Endocrine disrupting organotin compounds are potent inducers of adipogenesis in vertebrates. Mol Endocrinol. 20: 2141-2155. | ||
| Gardiner, D.M. and Susan V. Bryant (2006). Limb Regeneration. in “Fins into Limbs”, Brian Hall (ed.). Univ. Chicago Press. Chicago | ||
| Wong, B.J.F., Pandhoh, N., Truong, M.T., Diaz, S., Chao, K., Hou, S. and Gardiner, D.M. (2005). Identification of chondrocyte proliferation following laser irradiation, thermal injury, and mechanical trauma. Lasers in Surgery & Medicine. 37:89-96. | ||
| Gardiner, D.M. (2005). Ontogenetic decline of regenerative ability and the stimulation of human regeneration. Rejuvenation Res. 8:141-153. | ||
| Putta, S., Smith, J.J., Walker, J., Rondet, M., Weisrock, D.W., Monaghan, J., Samuels, A.K., Kump, K., King, D.C., Maness, N.J., Habermann, B., Tanaka, E., Bryant, S.V., Gardiner, D.M., Parichy, D.M. and Voss, S.R. (2004). From Biomedicine to Natural History Research: Expressed Sequence Tag Resources for Ambystomatid Salamanders. BMC Genomics 5:54-70. | ||
| Endo, T., Bryant, S.V., and Gardiner, D.M. (2004). A stepwise model system for limb regeneration. (2004). Dev. Biol. 270:135-145. | ||
| Bridges, C., Little, E., Gardiner, D., Petty, J., and Huckins, J. (2004). Assessing the toxicity and teratogenicity of pond water in North-Central Minnesota to amphibians. Environ. Sci. Pollution Res. 11:233-239. | ||
| Gardiner, D.M., Ndayibagira, A., Grun, F. and Blumberg, B. (2003). Deformed Frogs and Environmental Retinoids. Pure Applied Chem. 75:2263-2274 | ||
| Bryant, S.V., Endo, T. and Gardiner, D.M. (2002). Vertebrate limb regeneration and the origin of limb stem cells. Int. J. Dev. Biol. 46:887-896 | ||
| S. Randal Voss, J.J. Smith, D.M. Gardiner, and D.M. Parichyt (2001) Conserved Vertebrate Chromosome Segments in the Large Salamander Genome. Genetics. 158:735-746. | ||
| Carlson, M.R.J., Komine, Y., Bryant, S.V., and Gardiner, D.M. (2001). Expression of Hoxb13 and Hoxc10 in developing and regenerating axolotl limbs and tails. Dev. Biol. 229, 396-406. | ||
| Roy, S., Gardiner, D.M. and Bryant, S.V. (2000). Vaccinia as a tool for functional analysis in regenerating limbs: ectopic expression of Shh. Dev. Biol. 218, 199-205. | ||
| Burkhart, J.G., Ankley, G., Bell, H., Carpenter, H., Fort, D., Gardiner, D., Gardner, H., Hale, R., Helgen, J.C., Jepson, P., Johnson, D., Lannoo, M., Lee, D., Lary, J., Levey, R., Magner, J., Meteyer, C., Shelby, M.D., and Lucier, G. (2000). Strategies for assessing the implications of malformed frogs for environmental health. Envir. Health Perspectives. 108, 83-90. | ||
| Gardiner, D.M. and Hoppe, D.M. (1999). Environmentally-induced limb malformations in mink frogs (Rana septentrionalis). J. Exp. Zool. 284, 207-216 | ||
| Grants | DARPA, US Department of Defense, "Digit Regeneration in Mammals" | |
| Army Research Office, MURI, "Systems Biology of Regeneration" | ||
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Professional Societies |
Society for Developmental Biology American Society for Cell Biology American Association for the Advancement of Science |
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| Other Experience |
Assistant Dean for Corporate Relations School of Biological Sciences, UC Irvine 1999—2000 |
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Visiting Assistant Professor of Zoology and Developmental Biology UC Davis 1980—1982 |
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Visiting Assistant Professor of Biology Occidental College 1976—1980 |
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Associate Director of Marine Science Occidental College 1978—1980 |
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| Graduate Programs |
Developmental Biology and Genetics |
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| Research Center | Developmental Biology Center | |
| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=4614 | |
| Last updated | 02/13/2013 | |