Home | For Faculty | Help | About | Webmaster

Frank M LaFerla

Chancellor's Professor and Chair, Neurobiology and Behavior
School of Biological Sciences

Director, Institute for Memory Impairments and Neurological Disorders (UCI MIND)


PH.D., University of Minnesota

Phone: (949) 824-1232
Fax: (949) 824-7356
Email: laferla@uci.edu

University of California, Irvine
3212 Biological Sciences III
Mail Code: 4545
Irvine, CA 92697

picture of Frank M LaFerla

Research
Interests
Molecular biology of Alzheimer's disease and neurodegenerative disorders; Transgenic and Genetically-modified Animal Models, Presenilins and calcium signaling; Learning and memory
   
URLs neurobiology.uci.edu/faculty/laferla/
   
UCI MIND
   
UCI MIND FaceBook Page
   
Academic
Distinctions
Elected Member, American Neurological Association (2011); Fellow, American Association for the Advancement of Science (2010); UCI Academic Senate Daniel G. Aldrich Jr. Distinguished University Service Award (2010); Chancellor's Professor (2008); Promising Work Award, MetLife Foundation Awards for Medical Research (2006); UCI Faculty Innovation Award (2005); Zenith Fellow Award, Alzheimer's Association (2005); Chancellor's Fellow (2005-2008); Distinguished Mid-career Faculty Award for Research (2005); Ruth Salta Junior Investigator Achievement Award in Alzheimer’s Disease from the American Health Assistance Foundation (2001); Intracellular Imaging Inc. Ratio Imaging Award (1999); Young Scientist Travel Grant --Calgary, Italy (1995); F. M. Firby/Cancer Research Institute Postdoctoral Fellow (1990-93).
   
Research
Abstract
The neuropathological correlates of Alzheimer’s disease (AD) include amyloid-ß (Aß) plaques and tau-laden neurofibrillary tangles. Although it has been possible to develop transgenic mice that develop one of these lesions, it has proven to be quite challenging to develop mice with both histopathological lesions in the same mouse, in AD-relevant brain regions such as the hippocampus and cortex. To better model AD neuropathology, my lab utilized a novel approach to create a triple transgenic model of AD (3xTg-AD). Rather than crossing independent lines, we microinjected two transgenes (ßAPP and tau) into single-cell embryos from homozygous PS1M146V knockin mice, generating mice with the same genetic background. Compared to crossbreeding, the approach we used offers several major advantages. The integration of the ßAPP and tau transgenes at the same genetic locus renders it unlikely that either transgene will independently assort in subsequent generations. Therefore, this tight linkage coupled to the ‘knockin’ of the PS1 mutation indicates that the 3xTg-AD mice breed as readily as any single transgenic line, particularly because these mice have also been bred to homozygosity. Thus, deriving a large colony is straightforward, cost-effective, and does not require extensive genotyping of the progeny. Moreover, the easy propagation of this transgenic line facilitates their crossing to other transgenic or gene-targeted mice to assess the impact of other genotypes on the neuropathological or physiological phenotype. Lastly, another advantage to this approach is that multiple transgenes are introduced into an animal without altering or mixing the background genetic constitution. Thus, an important confounding variable is avoided, which may be a crucial parameter for behavioral, electrophysiological, and vaccine-based experiments


The 3xTg-AD mice develop both plaque and tangle pathology in AD-relevant brain regions. The 3xTg-AD mice develop extracellular Aß deposits prior to tangle formation, consistent with the amyloid cascade hypothesis. Despite equivalent overexpression of the human ßAPP and human tau transgenes, Aß deposition develops prior to the tangle pathology, consistent with the amyloid cascade hypothesis. In addition, these mice exhibit deficits in synaptic plasticity, including long-term potentiation (LTP) that occurs prior to extracellular Aß deposition and tau pathology, but is associated with intracellular Aß immunoreactivity. These studies support the view that synaptic dysfunction is a proximal defect in the pathobiology of AD, preceding extracellular plaque formation and neurofibrillary pathology. As these 3xTg-AD mice phenocopy critical aspects of AD neuropathology, this model will be useful in pre-clinical intervention trials, particularly because the efficacy of anti-AD compounds in mitigating the neurodegenerative effects mediated by both signature lesions can be evaluated.
   
Available Technologies
Publications Kitazawa, M., Cheng, D., Tsukamoto, M. R., Koike, M. A., Wes, P.D., Vailevko, V., Cribbs, D. H., and LaFerla, F. M. (2011). Blocking IL-1 signaling rescues cognition, attenuates tau pathology, and restores neuronal ß–catenin pathway function in an Alzheimer’s disease model. Journal of Immunology 187, 6539-6549.
   
  Neely, K. M., Green, K. N., and LaFerla, F. M. (2011). Presenilin is necessary for efficient proteolysis through the autophagy-lyososome system in a ?-secretase independent manner. Journal of Neuroscience 31, 2781-2791.
   
  Clinton, L. K., Blurton-Jones, M., Myczek, K., Trojanowski, J. Q., and LaFerla, F. M. (2010). Synergistic interaction between Aß, tau, and a-synuclein: acceleration of neuropathology and cognitive decline. Journal of Neuroscience 30, 7281-7289.
   
  Koike, M., Green, K. N., Blurton-Jones, M., and LaFerla, F. M. (2010). Oligomeic hypoperfusion differentially affects tau and amyloid-beta. American Journal of Pathology 177, 300-310
   
  Querfurth, H. W. and LaFerla, F. M. (2010). Alzheimer’s disease. New England Journal of Medicine 362, 329-344.
   
  Green, K.N., Kahshwji, H., Estrada, T., LaFerla, F.M. (2011) ST101 induces a novel 17kDa APP cleavage that precludes Aß generation in vivo. Annals of Neurology 69, 831-844.
   
  Blurton-Jones, M., Kitazawa, M., Martinez-Coria, H., Muller, F. J., Loring, J. F., Poon, W. W., Green, K. N., and LaFerla, F. M. (2009). Neural stem cells rescue cognition by increasing synaptic density in Alzheimer disease mice via a neurotrophic mechanism. Proc. Natl. Acad. Sci, USA, 106, 13594-13599.
   
  Green, K. N., Demuro, A., Akbari, Y., Hitt, B. D., Smith, I. F., Parker, I., and LaFerla, F. M. (2008). SERCA pump activity is physiologically regulated by presenilin and regulates Aß production. Journal of Cell Biology 181, 1107-1116.
   
  Green, K. N. and LaFerla, F. M. (2008). Linking calcium to Aß and Alzheimer’s disease. Neuron 59, 190-194.
   
Professional
Societies
Society for Neuroscience
American Society for the Advancement of Science
American Neurological Association
International Society for Stem Cell Research
   
Graduate Programs Neurobiology and Behavior

Interdepartmental Neuroscience Program

   
Research Centers Institute for Memory Impairments and Neurological Disorders
   
Center for the Neurobiology of Learning and Memory
   
Sue and Bill Gross Stem Cell Center
   
   
Link to this profile http://www.faculty.uci.edu/profile.cfm?faculty_id=3269
   
Last updated 02/17/2012