Nancy L. AllbrittonProfessor, Physiology & Biophysics |
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Research Interests |
Signal transduction, Single-cell assays, Quantitative cellular analyses, Analytical chemistry | |
| URL | Allbritton Lab | |
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Academic Distinctions |
1995 Searle Scholar Award 1995 Beckman Young Investigator Award 2003 UCI Midcareer Research Award 2004 College of Medicine Excellence in Teaching Award |
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| Appointments |
1988 Postdoctoral Fellowship, Herman Eisen, Dept. of Biology, M.I.T. 1989 - 1994 Postdoctoral Fellowship, Lubert Stryer, Dept. of Cell Biology and Neurobiology, Stanford University |
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Research Abstract |
There is tremendous interest in understanding how cells receive and process signals in order to carry out a given cellular response, a field known as cellular signal transduction. The research objective of the Allbritton Lab is to quantitatively measure cellular signaling networks in order to understand the relationships of these intracellular pathways in controlling cellular physiology. In order to elucidate these complex interrelationships, measurements on individual cells must be undertaken. The Allbritton lab has pursued this task by bringing to bear diverse techniques from biology, physics, chemistry, and engineering to develop new analytical techniques to identify molecular circuits within a single cell. New methods involving selective laser-induced ablation and highly sensitive microanalytical separations have been developed by the lab for single-cell applications. This research has involved a number of biological systems, but is particularly focused on studies of enzymatic activity involved in cellular signaling. Recent work has addressed quantifying the activity of enzymes downstream of calcium activation, especially protein kinase C and calcium/calmodulin-activated kinase II. Studies are also addressing single-cell enzyme activation within a variety of pathways involved in growth, development and survival. An additional focus area of the lab is the development of new microfluidic technologies for chemical analysis of single cells. Novel microscale devices are being designed and tested to overcome challenges related to the separation and detection of multiple cellular analytes from single cells. | |
| Publications | Meredith, G.D., Wu, H.Y., Allbritton, N.L., 2004. Targeted Protein Functionalization Using His-Tags. Bioconjugate Chem. 15:969-82. | |
| Soughayer, J.S., Wang, Y., Li, H., Cheung, S.H., Rossi, F.M., Stanbridge, E.J., Sims, E.S., Allbritton, N.L. 2004. Characterization of TAT-Mediated Transport of Detachable Kinase Substrates. Biochemistry. 43:8528-40. | ||
| Hu, S., Ren, X., Bachman, M., Sims, C.E., Li, G.P., Allbritton, N.L. 2004. Tailoring the Surface Properties of Poly(dimethylsiloxane) Microfluidic Devices. Langmuir. 20:5569-74. | ||
| Hu, S., Ren, X., Bachman, M., Sims, C.E., Li, G.P., Allbritton, N.L. 2004. Surface-Directed, Graft Polymerization within Microfluidic Channels. Anal. Chem. 76:1865-1870. | ||
| Li, H. Sims, C.E., Stanbridge, E.J., Allbritton, N.L. 2004. Quantitative Single-Cell Assay for Protein Kinase B Reveals Important Insights into the Biochemical Behavior of an Intracellular Substrate Peptide. Biochemistry. 43:1599-1608. | ||
| Wagner, J., Fall, C.P., Hong, F., Sims, C.E., Allbritton, N.L., Fontanilla, R.A., Moraru, I.I., Loew, L.M., Nuccitelli, R.A. 2004. A wave of IP3 production accompanies the fertilization Ca2+ wave in the egg of the frog, Xenopus laevis: theoretical and experimental support. Cell Calcium. 35:433-447. | ||
| McClain, M.A., Culbertson, C.T., Jacobson, S.C., Allbritton, N.L., Sims, C.E., Ramsey, J.M. 2003. Microfluidic Devices for the High Throughput Chemical Analysis of Cells. Anal. Chem. 75:5646-55. | ||
| Hu, S., Ren, X., Bachman, M., Sims, C.E., Li, G.P., Allbritton, N.L. 2003. Cross-Linked Coatings for Electrophoretic Separations in Poly(dimethylsiloxane) Microchannels. Electrophoresis. 524:3679-88. | ||
| Sims, C.E., Allbritton, N.L. 2003. Single Cell Kinase Assays: Opening a window onto cell behavior. Current Opin. Biotech. 14:1-6. | ||
| Han, F., Wang, Y., Sims, C.E., Bachman, M., Chang, R., Li, G.P., Allbritton, N.L. 2003. Fast Electrical Lysis of Cells for Capillary Electrophoresis. Anal. Chem. 75:3688-96. | ||
| Wang, Y., Hu, S., Li, H., Allbritton, N.L., Sims, C.E. 2003. Separation of mixtures of acidic and basic peptides at neutral pH. J. Chromatogr. A. 1004:61-70. | ||
| Graduate Programs |
Structural Biology and Molecular Biophysics Biomedical Engineering Chemical Engineering Chemistry Cell Biology |
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| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=2906 | |
| Last updated | 10/01/2005 | |
| (This faculty member is (or was) affiliated with UCI, but does not currently have an active appointment with UCI.) | ||