Donald F. SenearProfessor, Molecular Biology and Biochemistry |
|
|
Research Interests |
Regulatory Energetics and Protein-DNA Assemblies | |
| URL | darwin.bio.uci.edu/~faculty/senear/ | |
|
Research Abstract |
Our goal is to understand the fundamental mechanisms of transcriptional regulation at the level of macromolecular structure, function and interactions. Initiation of transcription, the primary point for control of gene expression, is regulated by the spontaneous assembly of regulatory protein and DNA containing complexes. Specific promoters are metabolically or developmentally regulated through coupling interactions between the site-specific protein-DNA interactions and protein-protein interactions, protein and DNA conformational transitions and allosteric transitions mediated by binding of regulatory ligands. Understanding the chemical basis for these macromolecular interactions provides the foundation to understand biological mechanisms of metabolic regulation, differentiation and development, and carcinogenesis. |
|
| Publications | Tretyachenko-Ladokhina, V, Ross, J.B.A. & Senear, D.F. (2002) "Operator Site Binding by the E. coli CytR Repressor Protein: Different Binding Modes for Different Operators Suggests a Role in Differential Gene Regulation", J. Mol. Biol. 316 (3), 531-546. | |
|
Gavigan S.A., Nguyen T., Nguyen N., Senear D.F. (1999) "Role of Multiple CytR Binding Sites on Cooperativity, Competition and Induction at the Escherichia coli udp Promoter" J. Biol Chem 274, 16010-16019. |
||
| Rusinova, E., Ross, J.B.A., Laue, T.M., Sowers, L.C. & Rhee, K.Y., Senear, D.F. & Hatfield, G.W. (1998) "Transcriptional Activation by a Ligand-induced Conformational Change in a Protein-DNA Complex" J. Biol. Chem. 273, 11257-11266. | ||
| Rusinova, E., Ross, J.B.A., Laue, T.M., Sowers, L.C. & Senear, D.F. (1997) "Linkage Between Operator Binding and Dimer to Octamer Assembly of Bacteriophage Lambda Repressor" Biochemistry 42, 12994-13003 | ||
| Barbier, C., Short, S & Senear, D.F. (1997) "The Allosteric Mechansim of Induction of CytR Controlled Gene Expression" J. Biol Chem. 272, 16962-16971. | ||
| Huang, Y.T. Rusinova, E., Ross, J.B.A. & Senear, D.F. (1997) "An Aromatic Stacking Interaction Between Subunits Helps Mediate DNA Sequence Specificity: Operator Site Discrimination by Phage Lambda cI Repressor ", J. Mol. Biol. 267, 403-417.. | ||
|
Professional Societies |
The Biophysical Society The Protein Society |
|
| Graduate Programs |
Mechanisms of Gene Expression Structural Biology and Molecular Biophysics Protein Engineering Biotechnology |
|
| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=2697 | |
| Last updated | 12/23/2002 | |