Susan V. Bryant
Research Professor, Developmental & Cell Biology
School of Biological Sciences
School of Biological Sciences
B.S., University of London, 1964
Ph.D., University of London, 1967
Ph.D., University of London, 1967
University of California, Irvine
Department of Developmental and Cell Biology
University of California, Irvine
Mail Code: 1430
Irvine, CA 92697
Department of Developmental and Cell Biology
University of California, Irvine
Mail Code: 1430
Irvine, CA 92697
Research Interests
Regeneration, stem cells, wound healing, growth, pattern formation, limb development, limb regeneration.
Academic Distinctions
Fellow, American Association for the Advancement of Science; Fellow, Association for Women in Science; Member, Independent Citizens Oversight Committee (ICOC) for the California Institute of Regenerative Medicine (CIRM); Former Member, NSF Directorate Biological Sciences Advisory Committee(BIOAC); Council member, California Council on Science and Technology (CCST)
Research Abstract
How to Regenerate a New Leg: What we can learn from salamanders.
Alone among vertebrates, urodele amphibians are able to regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. In current research, we are using assays derived from the extensive experimental history of regenerating limbs, to test the roles of several signaling molecules known to be essential for limb development. These assays are designed to examine the signals that initiate regeneration, those that are needed to establish a blastema, as well as those that are required for intercalary growth between the new limb tip and the amputation plane. In addition, we are taking a genomics approach to the isolation of genes needed for regeneration. A library of arrayed cDNAs made from limbs at different stages of regeneration will be screened with stage specific probes to identify genes expressed at different times in regeneration. We are especially interested in any novel factors that might be involved in transforming the differentiated limb stump into a blastema. The tools and knowledge are in place to attack complex systems, and understanding regeneration is likely to lead to new approaches and therapies for replacing or repairing lost, damaged or diseased parts of the body.
Alone among vertebrates, urodele amphibians are able to regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. In current research, we are using assays derived from the extensive experimental history of regenerating limbs, to test the roles of several signaling molecules known to be essential for limb development. These assays are designed to examine the signals that initiate regeneration, those that are needed to establish a blastema, as well as those that are required for intercalary growth between the new limb tip and the amputation plane. In addition, we are taking a genomics approach to the isolation of genes needed for regeneration. A library of arrayed cDNAs made from limbs at different stages of regeneration will be screened with stage specific probes to identify genes expressed at different times in regeneration. We are especially interested in any novel factors that might be involved in transforming the differentiated limb stump into a blastema. The tools and knowledge are in place to attack complex systems, and understanding regeneration is likely to lead to new approaches and therapies for replacing or repairing lost, damaged or diseased parts of the body.
Publications
Satoh, A., Cummings, G.M.C., Bryant, S.V. and Gardiner, D.M. (2010). Regulation of proximal-distal intercalation during limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Growth Diff. 52: 785-798.
Monaghan, J.R., Epp, L., Putta, S., Page, R.B., Walker, J.A., Beachy, C.K., Zhu, W., Pao, G.M., Verma, I.M., Hunter, T., Bryant, S.V., Gardiner, D.M., Harkins, T.T., and Voss, S.R. (2009). Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration. BMC Biology 7:1-19.
Satoh, A., Cummings, G.M.C., Bryant, S.V., and Gardiner, D.M. (2010). Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 337:444-457.
Smith, J.J., Putta, S., Zhu, W., Pao,. G., Harkins, T., Hunter, T., Verma, I., Bryant, S.V., Gardiner, D.M., and Voss, S.R. (2009). Genic Regions of a Large Salamander Genome Contain Long Introns and Novel Genes. BMC Genomics 10:19-29.
Satoh, A., Bryant, S.V. and Gardiner, D.M. (2008). Regulation of dermal fibroblast dedifferentiation and redifferentiation during wound healing and limb regeneration in the Axolotl. Dev. Growth & Diff. 50:743-754.
Satoh, A., Graham, G.M.C., Bryant, S.V. and Gardiner, D.M. (2008). Neurotrophic regulation of epidermal dedifferentiation during wound healing and limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 319:321-325
Satoh, A., Gardiner, D.M., Bryant, S.V. and Endo, T. (2007). Nerve-induced ectopic limb blastemas in the axolotl are equivalent to amputation-induced blastemas. Dev. Biol. 312:231-244
Gardiner, D.M. and Susan V. Bryant (2006). Limb Regeneration. in “Fins into Limbs”, Brian Hall (ed.). Univ. Chicago Press. Chicago.
Putta, S., Smith, J.J., Walker, J., Rondet, M., Weisrock, D.W., Monaghan, J., Samuels, A.K., Kump, K., King, D.C., Maness, N.J., Habermann, B., Tanaka, E., Bryant, S.V., Gardiner, D.M., Parichy, D.M. and Voss, S.R. (2004). From Biomedicine to Natural History Research: Expressed Sequence Tag Resources for Ambystomatid Salamanders. BMC Genomics 5:54-70.
Putta, S., Smith, J.J., Walker, J., Rondet, M., Weisrock, D.W., Monaghan, J., Samuels, A.K., Kump, K., King, D.C., Maness, N.J., Habermann, B., Tanaka, E., Bryant, S.V., Gardiner, D.M., Parichy, D.M. and Voss, S.R. (2004). From Biomedicine to Natural History Research: Expressed Sequence Tag Resources for Ambystomatid Salamanders. BMC Genomics 5:54-70.
Endo, T., Bryant, S.V., and Gardiner, D.M. (2004). A stepwise model system for limb regeneration. (2004). Dev. Biol. 270:135-145.
Bryant, S.V., Endo, T, and Gardiner, D.M.(2002) Vertebrate limb regeneration and the origin of limb stem cells. IJDB. 46:887-896
Gardiner, D.M., Endo, T. and Bryant, S.V. (2002). The molecular basis of amphibian limb regeneration: Integrating the old with the new. Sem. Cell Dev. Biol. 13:345-352.
Gardiner, D.M., Endo, T. and Bryant, S.V. (2002). The molecular basis of amphibian limb regeneration: Integrating the old with the new. Sem. Cell Dev. Biol. 13:345-352.
Carlson, M.R.J., Komine, Y., Bryant, S. V. and Gardiner, D. M (2001). Expression of Hoxb13 and Hoxc10 in Developing and Regenerating Axolotl Limbs and Tails, Dev Biol, 229, 396-406
Roy, S., Gardiner, D.M. and Bryant, S.V. (2000) Vaccinia as a Tool for Functional Analysis in Regenerating Limbs: Ectopic Expression of Shh. Dev Biol. 218, 199-205
Torok, M.A., Gardiner, D. M., Izpisua-Belmonte, J-C., and Bryant, S.V. (1999) Analysis of Sonic hedgehog expression in developing and regenerating axolotl limbs. J.exp Zool. 284, 197-206.
Torok, M.A., Gardiner, D.M., Shubin, N.H., Bryant, S.V. (1998) Regulation of HoxD expression in developing and regenerating axolotl limbs. Dev. Biol. 200, 225-233
Mullen, L., Torok, M. A., Bryant, S. V. and Gardiner, D. M. (1996). Nerve dependency of regeneration: Role of Dlx and FGF signalling in amphibian limb regeneration Development , 122, 3487-97.
Gardiner, D. M., Blumberg, B., Komine, Y. and Bryant, S. V. (1995). Regulation of HoxA expression in developing and regenerating axolotl limbs. Development, 121, 1731-1741
Bryant, S. V. and Gardiner, D. M. (1992). Retinoic acid, local cell-cell interactions, and pattern formation in vertebrate limbs. Dev. Biol. 152, 1-25.
Bryant, S., French, V. and Bryant, P. (1981). Distal regeneration and symmetry. Science 212, 993-1002.
French, V., Bryant, P.J. and Bryant, S.V. (1976). Pattern regulation in epimorphic fields. Science 193, 969-981.
Professional Societies
Society for Developmental Biology
American Society for Cell Biology
Fellow, American Association for the Advancement of Science
Fellow, Association for Women in Science
Council Member, California Council for Science and Technology
Board Member, California Institute for Regenerative Medicine
Other Experience
Vice Chancellor for Research
UC Irvine 2006—2010
UC Irvine 2006—2010
Principal Investigator
NSF ADVANCE Institutional Transformation Award 2001—2006
NSF ADVANCE Institutional Transformation Award 2001—2006
Dean
School of Biological Sciences 2000—2006
School of Biological Sciences 2000—2006
Chair
Department of Developmental and Cell Biology 1995—1997
Department of Developmental and Cell Biology 1995—1997
Program Director
Developmental Biology, National Science Foundation 1981—1982
Developmental Biology, National Science Foundation 1981—1982
Assistant Vice Chancellor for Plans and Programs
UC Irvine 1973—1975
UC Irvine 1973—1975
Research Centers
Developmental Biology Center
Sue and Bill Gross Stem Cell Research Center
Link to this profile
https://faculty.uci.edu/profile/?facultyId=2114
https://faculty.uci.edu/profile/?facultyId=2114
Last updated
01/23/2012
01/23/2012