Susan V. BryantResearch Professor, Developmental & Cell Biology |
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Research Interests |
Regeneration, stem cells, wound healing, growth, pattern formation, limb development, limb regeneration. | |
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Academic Distinctions | Fellow, American Association for the Advancement of Science; Fellow, Association for Women in Science; Member, Independent Citizens Oversight Committee (ICOC) for the California Institute of Regenerative Medicine (CIRM); Former Member, NSF Directorate Biological Sciences Advisory Committee(BIOAC); Council member, California Council on Science and Technology (CCST) | |
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Research Abstract |
How to Regenerate a New Leg: What we can learn from salamanders. Alone among vertebrates, urodele amphibians are able to regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. In current research, we are using assays derived from the extensive experimental history of regenerating limbs, to test the roles of several signaling molecules known to be essential for limb development. These assays are designed to examine the signals that initiate regeneration, those that are needed to establish a blastema, as well as those that are required for intercalary growth between the new limb tip and the amputation plane. In addition, we are taking a genomics approach to the isolation of genes needed for regeneration. A library of arrayed cDNAs made from limbs at different stages of regeneration will be screened with stage specific probes to identify genes expressed at different times in regeneration. We are especially interested in any novel factors that might be involved in transforming the differentiated limb stump into a blastema. The tools and knowledge are in place to attack complex systems, and understanding regeneration is likely to lead to new approaches and therapies for replacing or repairing lost, damaged or diseased parts of the body. |
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| Publications | Satoh, A., Cummings, G.M.C., Bryant, S.V. and Gardiner, D.M. (2010). Regulation of proximal-distal intercalation during limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Growth Diff. 52: 785-798. | |
| Monaghan, J.R., Epp, L., Putta, S., Page, R.B., Walker, J.A., Beachy, C.K., Zhu, W., Pao, G.M., Verma, I.M., Hunter, T., Bryant, S.V., Gardiner, D.M., Harkins, T.T., and Voss, S.R. (2009). Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration. BMC Biology 7:1-19. | ||
| Satoh, A., Cummings, G.M.C., Bryant, S.V., and Gardiner, D.M. (2010). Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 337:444-457. | ||
| Smith, J.J., Putta, S., Zhu, W., Pao,. G., Harkins, T., Hunter, T., Verma, I., Bryant, S.V., Gardiner, D.M., and Voss, S.R. (2009). Genic Regions of a Large Salamander Genome Contain Long Introns and Novel Genes. BMC Genomics 10:19-29. | ||
| Satoh, A., Bryant, S.V. and Gardiner, D.M. (2008). Regulation of dermal fibroblast dedifferentiation and redifferentiation during wound healing and limb regeneration in the Axolotl. Dev. Growth & Diff. 50:743-754. | ||
| Satoh, A., Graham, G.M.C., Bryant, S.V. and Gardiner, D.M. (2008). Neurotrophic regulation of epidermal dedifferentiation during wound healing and limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 319:321-325 | ||
| Satoh, A., Gardiner, D.M., Bryant, S.V. and Endo, T. (2007). Nerve-induced ectopic limb blastemas in the axolotl are equivalent to amputation-induced blastemas. Dev. Biol. 312:231-244 | ||
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Gardiner, D.M. and Susan V. Bryant (2006). Limb Regeneration. in “Fins into Limbs”, Brian Hall (ed.). Univ. Chicago Press. Chicago. Putta, S., Smith, J.J., Walker, J., Rondet, M., Weisrock, D.W., Monaghan, J., Samuels, A.K., Kump, K., King, D.C., Maness, N.J., Habermann, B., Tanaka, E., Bryant, S.V., Gardiner, D.M., Parichy, D.M. and Voss, S.R. (2004). From Biomedicine to Natural History Research: Expressed Sequence Tag Resources for Ambystomatid Salamanders. BMC Genomics 5:54-70. |
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| Endo, T., Bryant, S.V., and Gardiner, D.M. (2004). A stepwise model system for limb regeneration. (2004). Dev. Biol. 270:135-145. | ||
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Bryant, S.V., Endo, T, and Gardiner, D.M.(2002) Vertebrate limb regeneration and the origin of limb stem cells. IJDB. 46:887-896 Gardiner, D.M., Endo, T. and Bryant, S.V. (2002). The molecular basis of amphibian limb regeneration: Integrating the old with the new. Sem. Cell Dev. Biol. 13:345-352. |
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| Carlson, M.R.J., Komine, Y., Bryant, S. V. and Gardiner, D. M (2001). Expression of Hoxb13 and Hoxc10 in Developing and Regenerating Axolotl Limbs and Tails, Dev Biol, 229, 396-406 | ||
| Roy, S., Gardiner, D.M. and Bryant, S.V. (2000) Vaccinia as a Tool for Functional Analysis in Regenerating Limbs: Ectopic Expression of Shh. Dev Biol. 218, 199-205 | ||
| Torok, M.A., Gardiner, D. M., Izpisua-Belmonte, J-C., and Bryant, S.V. (1999) Analysis of Sonic hedgehog expression in developing and regenerating axolotl limbs. J.exp Zool. 284, 197-206. | ||
| Torok, M.A., Gardiner, D.M., Shubin, N.H., Bryant, S.V. (1998) Regulation of HoxD expression in developing and regenerating axolotl limbs. Dev. Biol. 200, 225-233 | ||
| Mullen, L., Torok, M. A., Bryant, S. V. and Gardiner, D. M. (1996). Nerve dependency of regeneration: Role of Dlx and FGF signalling in amphibian limb regeneration Development , 122, 3487-97. | ||
| Gardiner, D. M., Blumberg, B., Komine, Y. and Bryant, S. V. (1995). Regulation of HoxA expression in developing and regenerating axolotl limbs. Development, 121, 1731-1741 | ||
| Bryant, S. V. and Gardiner, D. M. (1992). Retinoic acid, local cell-cell interactions, and pattern formation in vertebrate limbs. Dev. Biol. 152, 1-25. | ||
| Bryant, S., French, V. and Bryant, P. (1981). Distal regeneration and symmetry. Science 212, 993-1002. | ||
| French, V., Bryant, P.J. and Bryant, S.V. (1976). Pattern regulation in epimorphic fields. Science 193, 969-981. | ||
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Professional Societies |
Society for Developmental Biology American Society for Cell Biology Fellow, American Association for the Advancement of Science Fellow, Association for Women in Science Council Member, California Council for Science and Technology Board Member, California Institute for Regenerative Medicine |
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| Other Experience |
Vice Chancellor for Research UC Irvine 2006—2010 |
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Principal Investigator NSF ADVANCE Institutional Transformation Award 2001—2006 |
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Dean School of Biological Sciences 2000—2006 |
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Chair Department of Developmental and Cell Biology 1995—1997 |
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Program Director Developmental Biology, National Science Foundation 1981—1982 |
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Assistant Vice Chancellor for Plans and Programs UC Irvine 1973—1975 |
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| Research Centers | Developmental Biology Center | |
| Sue and Bill Gross Stem Cell Research Center | ||
| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=2114 | |
| Last updated | 01/23/2012 | |